StemPro hESC SFM is a fully defined, serum- and feeder-free medium (SFM) specially formulated for the growth and expansion of human embryonic stem cells (hESCs).

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hESC SFM key features

  • Enables excellent expansion of hESCs
  • Maintains pluripotency and normal morphology/karyotype of hESCs
  • Maintains differentiation capability of hESCs
  • Results in gene expression profiles comparable to classical media
  • Each lot is performance-qualified using PCR
  • No or little adaptation required from feeder cultures
  • Eliminates variability associated with feeder-dependent cultures


Tested hESC lines

StemPro hESC SFM has been extensively tested and proven to maintain pluripotency in a growing list of hESC lines including:

BG01BG02BG03
H1H9BG01V
HUES9HUES6 
CyT49Cyth203 
Rhesus Monkey ES cells (line R366.4)
fluorescent microscopy image of hESCs stained with SSEA4 grown in StemPro hESC SFM

SSEA4 staining of hESCs grown in STEMPRO hESC SFM

fluorescent microscopy image of hESCs stained with Tra-1-81 grown in StemPro hESC SFM

Tra-1-81 staining of hESCs grown in STEMPRO hESC SFM

phase contrast image of BG02 cells grown in StemPro hESC SFM

BG02 cells grown in STEMPRO hESC SFM

phase contrast image of BG02 cells grown in StemPro hESC SFM

BG02 cells grown in STEMPRO hESC SFM


Geltrex Matrix for hESC culture

Geltrex Reduced Growth Factor Basement Membrane Matrix is designed for use with StemPro hESC SFM as a matrix to support hESC growth. Geltrex matrix has a consistently high lot-to-lot protein concentration and functional testing is performed on each lot.

Read about tips for hESC adaptation to StemPro hESC SFM and using Geltrex matrix


hESC SFM ordering information

hESC SFM resources

Protocols

For Research Use Only. Not for use in diagnostic procedures.

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