StemPro hESC SFM

StemPro hESC SFM – a fully-defined, serum- and feeder-free medium (SFM) specially formulated for the growth and expansion of human embryonic stem cells (hESCs)

Tips for hESC Adaptation to StemPro hESC SFM

  • From MEF conditioned media
  • From other serum and feeder-free media

Serum- and Feeder-Free Medium

  • Enables superior expansion of hESCs
  • Maintains pluripotency and normal morphology / karyotype of hESCs
  • Maintains differentiation capability of hESCs
  • Provides gene expression profiles comparable to classical media
  • Each lot is performance qualified using PCR
  • No or little adaptation required from feeder cultures
  • Eliminates variability associated with feeder-dependent cultures.
     
S SEAY hESC SFMTra 1 81 hESCBG02 hESC SFMBG02 Cells Stempro
SSEA4 staining of hESCs grown in STEMPRO® hESC SFMTra-1-81 staining of hESCs grown in STEMPRO® hESC SFMBG02 cells grown in STEMPRO® hESC SFMBG02 cells grown in STEMPRO® hESC SFM

Extensively tested

StemPro hESC SFM has been extensively tested and proven to maintain pluripotency in a growing list of hESC lines including:

BG01BG02BG03
H1H9BG01V
HUES9HUES6 
CyT49Cyth203 
Rhesus Monkey ES cells (line R366.4)

Geltrex Matrix for hESC culture

Geltrex Reduced Growth Factor Basement Membrane Matrix is designed for use with STEMPRO hESC SFM as a matrix to support hESC growth. Geltrex has a consistently high lot to lot protein concentration and functional testing is performed on each lot.