StemPro Human Adipose-Derived Stem Cell Kit

• Low passage—ADSCs passaged once after isolation
• Expanded in MesenPRO RS Medium—a reduced-serum (2%) medium that reduces ADSC doubling times
• Contains human ADSCs and MesenPRO RS Medium—components help ensure ADSC growth and expansion
• Demonstrate similar phenotypic and functional characteristics to bone marrow-derived mesenchymal stem cells
• Can be expanded to 4–5 passages before they lose their ability to grow or differentiate into all potential phenotypes

Flow cytometry results of ADSC cell surface markers at passage 2 indicate the cell population is consistent with a high-quality mesenchymal stem cell (MSC) phenotype and lacks contaminating hematopoietic or endothelial cells (Figure 1).

The detected positive markers—CD29, CD44, CD73, CD90, CD105, CD166—are all well-established MSC identifiers. Their presence suggests that the cells possess classic mesenchymal characteristics:

• CD29 (integrin β1)—adhesion molecule; typical of MSCs
• CD44—hyaluronan receptor; associated with MSC identity and migration
• CD73 (5′-nucleotidase)—MSC marker
• CD90 (Thy-1)—common MSC surface antigen linked to stemness
• CD105 (endoglin)—MSC marker involved in TGF-β signaling
• CD166 (ALCAM)—adhesion molecule enriched in mesenchymal cells

A strong signal for these markers indicates a pure, undifferentiated, adherent mesenchymal stem cell population with expected immunophenotype.

The absence of CD14, CD31, CD45, and Lin1 demonstrates that the culture is not contaminated with immune or endothelial cells:

• CD14—monocyte/macrophage marker
• CD31 (PECAM-1)—endothelial cell marker
• CD45—pan-leukocyte (hematopoietic) marker
• Lin1 (Lineage cocktail)—mix of hematopoietic lineage markers

Negative expression of these markers confirms the cells are not hematopoietic, not endothelial, and do not represent other differentiated immune lineages.

Each vial of ADSCs contains cells that can differentiate into multiple mature cell phenotypes in vitro including adipocytes, osteoblasts, and chondrocytes (Figure 2). In vitro differentiation into non-mesenchymal cell types, such as neuronal and glial progenitors, hepatocytes and vascular endothelial progenitors, cardiomyocytic, pancreatic, and epithelial phenotypes has also been described. In addition, ADSCs secrete pro-angiogenic, immunomodulatory, and anti-apoptotic factors. ADSCs can be used for studies of adult stem cell differentiation, tissue engineering, and potential future clinical applications or as cells amenable to delivery of recombinant DNA constructs.