Designed for manual passaging without the need for enzymatic dissociation, the StemPro EZPassage Disposable Stem Cell Passaging Tool helps enable rapid colony fragmentation while preserving the morphology, viability, and pluripotent state of hESCs and hiPSCs. The EZPassage tool helps support reproducible subculturing through minimized handling and reduced processing time.

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EZPassage tool key features

  • Device for manual passaging of cells
  • Makes cutting stem cell colonies easier, setting the stage for more reliable stem cell research
  • Made of a proprietary cell-culture safe inert material
  • Ready for-use and packaged individually in gamma-irradiated sleeves
  • Uniform colony slicing—cuts hESC colonies into uniform sized pieces for reproducible and optimal passaging
  • Outperforms existing manual and enzymatic passaging methods
  • Takes under 1 minute to passage a 6-well plate
  • No extensive training is required to learn how to passage hESCs with the EZPassage tool


How to use the EZPassage tool for cell passaging?

procedural schematic for using the STEMPRO EZPassage tool to cut hESC colonies
  1. Under a laminar-flow hood, inspect the culture and remove any differentiated areas if present.
  2. Remove the packaging of the EZPassage tool and hold the culture dish in one hand.
  3. First rolling pass: With the tool in contact with the plate surface (still containing medium), roll the tool across the dish in one direction (left to right), applying enough pressure to engage the roller blades.
  4. Second rolling pass: Rotate the dish 90° and roll again in the new direction. This produces a grid-pattern cut across the colonies, generating uniformly sized colony fragments.
  5. Using the serological pipette, add fresh medium to the dish and gently flush the plate surface to dislodge the cut fragments into suspension.
  6. Transfer the colony fragments to a conical tube; spin briefly (e.g., 1,000 rpm ≈ 200 × g for 2 minutes) or allow gravity-settling.
  7. Aspirate supernatant carefully to remove single cells or unwanted feeder cells if present and resuspend the colony fragments in fresh medium.
  8. Seed the colony fragments onto the prepared new plate at the desired split ratio (e.g., 1:2 to 1:4) and place in incubator. Avoid moving the plate for at least 24 h to allow attachment.
  9. Discard the EZPassage tool—do not reuse.


Ordering information for EZPassage tool and related reagents

EZPassage tool resources
Protocols

For Research Use Only. Not for use in diagnostic procedures.

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