StemPro MSC SFM is the first serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (hMSCs).

The ability to scale up hMSC production will be critical for any viable cell therapy. StemPro MSC SFM enables hMSC scale up from 1 million to 1 billion cells using 85% less medium, 92% less surface area, 16% less time, 25% less passaging and less effort (less plates, passaging and no pre-qualification of FBS) versus classical medium (DMEM + 10% MSC-Qualified FBS).

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MSC SFM key features

  • Excellent efficiency of hMSC expansion at high cell densities—less medium, surface area, and time
  • Better quality cells—primitive phenotype, retained hMSC surface marker expression, normal gene expression profiles, and maintained CFU-F and tri-lineage mesoderm differentiation potential beyond passage 5
  • Batch-to-batch consistency with each lot—produced under cGMP and qualified using a hMSC performance assay
  • Little to no adaptation required from serum-supplemented medium


hMSCs with a primitive phenotype grown in StemPro MSC SFM

Human MSCs grown in StemPro MSC SFM exhibit a primitive morphology compared to control culture (Figure 1).

While hMSCs grown in classical medium have a flattened cell morphology and reach confluency between 1.0–3.0 x 104 cells/cm2, hMSCs grown in StemPro MSC SFM have a much smaller, spindle-shaped morphology and can reach densities >1.0 x 105 cells/cm2.

two side-by-side microscopy images of hMSCs

Figure 1. hMSCs with a primitive phenotype grown in StemPro MSC SFM. Cell morphology of hMSCs when grown in StemPro MSC SFM or classical medium (DMEM + 10% MSC-Qualified FBS).


hMSCs differentiation potential in StemPro MSC SFM

hMSCs cultured in StemPro MSC SFM retained the tri-lineage mesoderm differentiation potential (adipocyte, chondrocyte, and osteoblast) beyond passage 5 (Figure 2).

microscopy images of hMSC differentiated into adipocytes, chondrocytes, and osteoblasts

Figure 2. hMSC differentiation potential over long-term passaging in StemPro MSC SFM. hMSCs cultured in StemPro MSC SFM (passage 5) were seeded into adipogenic, chondrogenic, or osteogenic differentiation medium for 14 days, revealing adipocytes (Oil Red O–lipid stain), chondrocytes (Alcian Blue–glycosaminoglycan stain), and osteoblasts (Alkaline Phosphatase–cell surface glycoprotein stain).


CELLstart substrate for serum-free hMSC culture

CELLstart substrate is designed for use with StemPro MSC SFM to support hMSC attachment when growing hMSCs under serum-free conditions. CELLstart substrate is the first xeno-free substrate for stem cell culture, is produced under cGMP and offers consistent lot-to-lot performance.


Ordering information

MSC SFM resources

Protocols

For Research Use Only. Not for use in diagnostic procedures.

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