Xeno-free media specifically formulated for the growth and expansion of human mesenchymal stem cells (hMSC) and human adipose-derived stem cells (hADSC)
- Supports hMSC and hADSC expansion for multiple passages
- Maintains spindle-shaped morphology
- Maintains multilineage mesoderm differentiation potential
- Supports primary isolation of hMSCs
- StemPro® MSC SFM XenoFree is cGMP compliant facilitating regulatory review/approval
Supports hMSC expansion
Expansion of hMSCs using StemPro MSC SFM XenoFree on CELLstart substrate-coated flasks is comparable to classical medium (DMEM + 10% FBS) (Figure 1).
Figure 1. Expansion of MSCs in StemPro MSC SFM XenoFree. Human Bone Marrow-derived MSCs (BM-MSCs) cultured in StemPro® MSC SFM XenoFree on CELLstart-coated flasks reveal a similar growth rate compared to DMEM + 10% MSC-Qualified FBS with long-term multi-passage capability
Better quality cells—spindle-shaped morphology
hMSCs grown in StemPro MSC SFM XenoFree medium on CELLstart substrate-coated flasks exhibit a less flattened, spindle-shaped morphology (Figure 2).
Maintains multilineage differentiation potential
hMSCs cultured in StemPro® MSC SFM XenoFree retain their tri-lineage mesoderm differentiation potential (Figure 3).
Primary Isolation of hMSCs
Purified bone marrow mononuclear cells cultured in StemPro MSC SFM XenoFree supplemented with low level pooled human AB serum (2.5%) supported primary BM-MSC expansion with continual propagation (after Passage 0) under serum- and xeno-free conditions (Figure 4).
Figure 4. Primary Human BM-MSC Expansion. Ficoll-purified BM-MNCs cultured in StemPro® MSC SFM XenoFree (on CELLstart-coated flasks) supplemented with low level pooled human AB serum (2.5%) supported primary BM-MSC expansion with continual propagation (after P0) under serum- and xeno-free conditions.
For Research Use Only. Not for use in diagnostic procedures.