Genetic instability is a known issue during long-term stem cell culture. The ability to quickly and continuously monitor your cell culture for genomic integrity is critical to allow you to move forward confidently with your research.
Through our KaryoStat+ Karyotyping Service, we offer an effective alternative to typical G-banded karyotyping that leverages the array-based Applied Biosystems CytoScan HT-CMA 96F Assay Kit, which offers whole-genome coverage for accurate and cost-effective detection of chromosomal abnormalities in human primary and stem cell populations.
|KaryoStat+ Service vs typical G-banding|
|Service||KaryoStat+ Karyotyping Service||Karyotyping by G-banding|
|Timelines||≤3 weeks||Over 3 weeks|
|Sample format||Frozen cell pellet||Live culture required (early metaphase)|
|Resolution||>1 Mb||>5–10 Mb|
Long-term culture of human embryonic stem cell (hESCs) lines and reprogramming of induced pluripotent stem cells (iPSCs) have been associated with the accumulation of chromosomal abnormalities and copy number variation. Thus, the ability to effectively verify the absence of major chromosomal aberrations is a critical quality control step in your stem cell research.
Figure 1. The KaryoStat service is an array-based alternative to G-banding that offers whole-genome coverage for accurate detection of chromosomal aberrations.
As part of the KaryoStat Karyotyping Service, you will receive a comprehensive report, including a whole genome view (Figure 2) that visually calls out detected chromosomal aberrations in your sample. In addition, you will receive a data file with the full details of the chromosomal aberrations in your submitted sample.
Figure 2. Example of whole genome view, provided as part of the KaryoStat Karyotyping Service report. The whole genome view displays all somatic and sex chromosomes in one frame along with an indication of copy number. The right y-axis depicts the signal intensities of the probes on the microarray, the value indicates the copy number status: normal copy number state (CN=2), a chromosomal gain (CN=3) or a chromosomal loss (CN=1) for all somatic chromosomes.
For Research Use Only. Not for use in diagnostic procedures.