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Transfection tips for cancer cells

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What did the comedian and the cancer researcher have in common? They both needed help with their delivery. Here are three tips and tricks to help improve transfection of cancer cells.

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Decreasing the serum content of the culture medium (to <10%) at the time of transfection is acceptable, but replace with complete growth medium within 4–24 hr posttransfection.

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Antibiotics can be used during transfection.

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Prior to flow cytometry, visualize cells under a brightfield microscope to verify dissociation following incubation with Gibco TrypLE reagent.

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