Transferring your existing HPLC or UHPLC method from one instrument to another doesn’t have to be stressful. By following a few simple guidelines and researching which method or system changes are acceptable, method transfer can actually be a smooth process.

All you need is the right technical solutions and to know which parameters — like the gradient delay volume — differ between your current and new system.

Our industry-leading Thermo Scientific Vanquish HPLC and UHPLC Systems offer you tailor-made hardware and software solutions like adjustable gradient delay volume (GDV), custom injection programs, and multiple thermostatting modes.

These advanced solutions specifically created by our expert liquid chromatographers will help make your LC method transfer journey faster and easier.

Learn more about what hardware parameters can impact your method transfer.

When transferring methods between HPLC instruments made by different vendors, you need to consider that every system on the market has its own unique parameters, such as gradient delay and extra column volumes, plus column and solvent temperature control. A mismatch between any of these parameters can alter analyte selectivity, retention time, and peak shapes, all of which may compromise the transferred method’s suitability.

Our Vanquish LC Systems offer you a variety of unique solutions like a tunable gradient delay volume to help reduce the likelihood of any major transferability issues. Taking steps to reduce the chance of transferability issues means you can transfer HPLC methods without having to revalidate, making your method transfer journey even easier.

Conserves retention time

A tunable GDV that physically mimics the delay volume of your original system to avoid retention time shifts, altered peak shapes, and selectivity.

Download technical note

Maintains analyte selectivity

Multiple column thermostatting modes and eluent preheating options to offset changes in analyte retention time and selectivity by matching the separation temperature conditions.

Download white paper

Sustains peak shape & gradient profile

Ability to mitigate different mixing behaviors and dispersion volumes with custom injection programs.

Download application note

Preserves peak quality

Conserves peak shape by neutralizing strong sample solvent effects that can occur from a mismatch in sample and mobile phase elution strength.

Download application notes

Flexible software options

All Vanquish LC method transfer features are available with the Thermo Scientific Chromeleon Chromatography Data Systems. Third-party integrations are available to accommodate labs standardized on another CDS.

Learn more about Chromeleon CDS

Conserves retention time

A tunable GDV that physically mimics the delay volume of your original system to avoid retention time shifts, altered peak shapes, and selectivity.

Download technical note

Maintains analyte selectivity

Multiple column thermostatting modes and eluent preheating options to offset changes in analyte retention time and selectivity by matching the separation temperature conditions.

Download white paper

Sustains peak shape & gradient profile

Ability to mitigate different mixing behaviors and dispersion volumes with custom injection programs.

Download application note

Preserves peak quality

Conserves peak shape by neutralizing strong sample solvent effects that can occur from a mismatch in sample and mobile phase elution strength.

Download application notes

Flexible software options

All Vanquish LC method transfer features are available with the Thermo Scientific Chromeleon Chromatography Data Systems. Third-party integrations are available to accommodate labs standardized on another CDS.

Learn more about Chromeleon CDS

Adjusting the GDV of your HPLC or UHPLC system is crucial to successfully transferring your existing LC method from one system to another.

The Thermo Scientific Vanquish Core HPLC System autosampler features an integrated metering device as part of the flow path and enables you to tune the GDV of the system from 0-230 µL without re-plumbing or requalifying the system. An optional Method Transfer Kit allows you to adjust the GDV up to 430 µL.

Take our virtual demo and see how you can refine separations to match the originating system by simply adjusting the GDV.

Vanquish customers with a block set
“We performed a method translation from a dated HPLC method to UHPLC on the Vanquish. The new method allows this test to be performed within one shift instead of across multiple shifts/personnel.”
— Nicholas Green | HIKMA
I #LoveMyVanquish UHPLC. It helps vanquish long methods when converting our HPLC methods to UHPLC using Chromeleon software.
— Edwin Srisan | Analytical Food Labs

Want more help guides and resources to aid your method transfer? See our full list of HPLC and UHPLC resources and method transfer calculators.

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