Fast SYBR® Green Master Mix is designed to deliver highly sensitive and reproducible PCR results on Fast-capable real-time PCR instruments in less than half the time of standard real-time PCR. The Master Mix contains all the components needed for SYBR Green-based real-time PCR (except template and primers) in a convenient 2X formulation that minimizes nonspecific PCR products. Fast SYBR Green Master Mix and Applied Biosystems Fast Real-Time PCR Systems enable researchers to save time and increase sample throughput without compromising the quality of real-time PCR analysis.
Detect Targets Over Broad Range of Template Concentrations
Minimized Nonspecific Amplification Without Compromised Sensitivity
Figure 1. Fast SYBR® Green Master Mix Delivers High Sensitivity. RNase P target amplified from 3.3−33 pg of human genomic DNA (gDNA), which corresponds to 1−10 copies of target, in 48 replicate reactions using the Applied Biosystems StepOnePlus™ Real-Time PCR System.
RT-PCR products amplified with PGRMC1-specific primers and either the Fast SYBR Green Master Mix or one of two other commercial master mixes were compared. Only Fast SYBR Green Master Mix demonstrated target specific amplification using as little as 0.1 pg cDNA with no primer-dimer formation or nonspecific amplification (data not shown).
Reliable Quantitation Distinguishing Less than 2-Fold Difference
- Fast SYBR® Green Master Mix: Real-Time PCR Master Mix Designed for Speed. (2007) Applied Biosystems Product Bulletin, Publication 136PB11-01