After cells are isolated from tissue or differentiated from pluripotent precursors, the resulting population needs to be characterized to confirm whether the target population has been obtained. This chapter lists cell-type specific antibody markers commonly used for immunocytochemical (ICC) and flow cytometric analysis of neural subtypes.

Cell-type Specific Antibodies for Characterizing Neural Subtypes

Cell type Antigen Type ICC dilution*
Neural stem cellsSox1Goat IgG10 μg/mL

Sox2Mouse IgG2 μg/mL

NestinMouse IgG1:500

CD133Rabbit IgG1:100
Neuronal progenitors Neurons (Pan)MAP2Mouse IgG1:200

HuC/DlMouse IgG10 μg/mL

NFMouse IgG1:100

NCAMMouse IgG1:50

βIII tubulinMouse IgG1:2,000

DcxRabbit IgG1:200
Dopaminergic neuronsTHRabbit IgG1:1,000
MotoneuronsIsl1Mouse IgG1:50

HB9Mouse IgG1:50
GABAergic/Glutaminergic neuronsGABARabbit IgG1:2,000
Cholinergic neuronsChATGoat IgG1:100
Astrocyte progenitors AstrocytesCD44Mouse IgG1:50

GFAPRat IgG1:100

GFAPRabbit IgG1:200
Oligodendrocyte progenitors OligodendrocytesGalCMouse IgG1:200

NG2Mouse IgG1:200

A2B5Mouse IgM1:2,000

O4Mouse IgM1:50
ProliferationKi67Rabbit IgG1:50

Isotype controlMouseIgM and IgGUse as is

RabbitIgGUse as is

* These are recommended starting concentrations for ICC applications; optimal working concentrations must be determined empirically.
LT159                    17-Mar-2011