Invitrogen

IL-13 Monoclonal Antibody (eBio13A), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

17 Published Figures

23 References

View all (46) IL-13 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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IL-13 Antibody in Flow Cytometry (Flow) — CD4+ BALB/c splenocytes were stimulated for 10-days under Th2 polarizing conditions. Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) for 5 hours. Following restimulation, cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and then stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and 0.25 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) (left) or 0.25 µg of Anti-Mouse IL-13 PE-Cyanine7 (right). Viable cells, as determined by Fixable Viability Dye eFluor® 660 (Product # 65-0864-14), were used for analysis.

IL-13 Antibody — Figure 6 OX40L on ILC2s Orchestrates Adaptive Type 2 Immunity to Allergens (A-D) Mice of the specified genotypes were treated with PBS or papain (Pap) (i.n., day 0 and 1), followed by quantification of lung Th2, GATA3 + Treg, and GATA3 - Treg cells on day 5 (A-C). Whole lung cell suspensions were re-stimulated with PMA and ionomycin, followed by quantification of IL-13 + Th2 cells by intracellular staining (D). (E-G) Mice were treated with papain on days 0, 1, 14, and 21, followed by quantification on day 24 of lung eosinophils (E), and detection (F) and quantification (G) of RELMalpha + M2 alveolar (CD45 + SiglecF + CD11c + CD11b - F4/80 + ) macrophages (MPhi). (H and I) Mice were treated with A. alternata ( A.alt ) (i.n., days 0 and 1), followed by quantification on day 9 of lung Th2, GATA3 + Treg, and GATA3 - Treg cells (H) and serum IgE concentration (I). Bar graphs indicate mean (+-SEM). (A)-(D), ANOVA, two repeat experiments; (E), ANOVA, three repeat experiments; (F) and (G), ANOVA, two repeat experiments, representative gate shown in (F); (H) and (I), ANOVA, two repeat experiments. ** p <= 0.01, *** p <= 0.001, **** p <= 0.0001. See also Figure S7 . {TM}

Product Details

25-7133-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.5 µg/test	View 23 publications 23 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBio13A
Conjugate	PE-Cyanine7 Alexa Fluor 488 APC-eFluor 780 Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 660 Functional Grade PE PE-eFluor 610 PerCP-eFluor 710 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2573530

Product Specific Information

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio13A antibody has been tested by intracellular staining of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dephosphorylated Polymerase I and Transcript Release Factor Prevents Allergic Asthma Exacerbations by Limiting IL-33 Release.

Frontiers in immunology

Ni Y,Hao J,Hou X,Du W,Yu Y,Chen T,Wei Z,Li Y,Zhu F,Wang S,Liang R,Li D,Lu Y,Liao K,Li B,Shi G

25-7133 was used in Flow cytometry/Cell sorting to investigate the role of Polymerase I and Transcript Release Factor in IL-33 release, and asthma pathogenesis.

Mon Mar 21 00:00:00 EDT 2022

Major histocompatibility complexes are up-regulated in glomerular endothelial cells via activation of c-Jun N-terminal kinase in 5/6 nephrectomy mice.

British journal of pharmacology

Zhu D,Tang Q,Yu B,Meng M,Liu W,Li J,Zhu T,Vanhoutte PM,Leung SWS,Zhang Y,Shi Y

25-7133 was used in Flow cytometry/Cell sorting to explore the mechanism underlying the up-regulation of major histocompatibility complex (MHC) proteins in glomerular endothelial cells in 5/6 nephrectomy mice.

Sun Nov 01 00:00:00 EDT 2020

T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.

Cell reports

Yan J,Pandey SP,Barnes BJ,Turner JR,Abraham C

25-7133 was used in Flow cytometry/Cell sorting to identify a previously undefined key role for T cell-intrinsic IRF5. In mice, IRF5 in CD4+ T cells promotes Th1- and Th17-associated cytokines and decreases Th2-associated cytokines.

Tue Jun 30 00:00:00 EDT 2020

Isolation and functional characterisation of lamina propria leukocytes from helminth-infected, murine small intestine.

Journal of immunological methods

Webster HC,Andrusaite AT,Shergold AL,Milling SWF,Perona-Wright G

25-7133 was used in Flow cytometry/Cell sorting to describe an optimised protocol for isolating LP leukocytes from the small intestine of H.polygyrus-infected mice.

Sat Feb 01 00:00:00 EST 2020

Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice.

Nature communications

Wang G,Xu J,Zhao J,Yin W,Liu D,Chen W,Hou SX

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes.

Nature communications

Galle-Treger L,Sankaranarayanan I,Hurrell BP,Howard E,Lo R,Maazi H,Lewis G,Banie H,Epstein AL,Hu P,Rehan VK,Gilliland FD,Allayee H,Soroosh P,Sharpe AH,Akbari O

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Tue Feb 12 00:00:00 EST 2019

Ym1 induces RELMα and rescues IL-4Rα deficiency in lung repair during nematode infection.

PLoS pathogens

Sutherland TE,Rückerl D,Logan N,Duncan S,Wynn TA,Allen JE

25-7133 was used in Flow cytometry/Cell sorting to show that Ym1 and RELMα exhibit time and dose-dependent interactions that determines the outcome of lung repair during nematode infection.

Thu Nov 01 00:00:00 EDT 2018

Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation.

Genome medicine

Pramanik J,Chen X,Kar G,Henriksson J,Gomes T,Park JE,Natarajan K,Meyer KB,Miao Z,McKenzie ANJ,Mahata B,Teichmann SA

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Wed Oct 24 00:00:00 EDT 2018

Expansion of commensal fungus Wallemia mellicola in the gastrointestinal mycobiota enhances the severity of allergic airway disease in mice.

PLoS pathogens

Skalski JH,Limon JJ,Sharma P,Gargus MD,Nguyen C,Tang J,Coelho AL,Hogaboam CM,Crother TR,Underhill DM

25-7133 was used in Flow cytometry/Cell sorting to investigate the effects of the population expansion of commensal fungus Wallemia mellicola without overgrowth of the total fungal community.

Sat Sep 01 00:00:00 EDT 2018

Autoimmune Th17 Cells Induced Synovial Stromal and Innate Lymphoid Cell Secretion of the Cytokine GM-CSF to Initiate and Augment Autoimmune Arthritis.

Immunity

Hirota K,Hashimoto M,Ito Y,Matsuura M,Ito H,Tanaka M,Watanabe H,Kondoh G,Tanaka A,Yasuda K,Kopf M,Potocnik AJ,Stockinger B,Sakaguchi N,Sakaguchi S

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

Concomitant suppression of T_H2 and T_H17 cell responses in allergic asthma by targeting retinoic acid receptor-related orphan receptor γt.

The Journal of allergy and clinical immunology

Na H,Lim H,Choi G,Kim BK,Kim SH,Chang YS,Nurieva R,Dong C,Chang SH,Chung Y

25-7133 was used in Flow cytometry/Cell sorting to determine the role of the TH17 cell pathway in regulating TH2 cell responses in allergic asthma.

Fri Jun 01 00:00:00 EDT 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Nippostrongylus brasiliensis infection leads to impaired reference memory and myeloid cell interference.

Scientific reports

Brombacher TM,De Gouveia KS,Cruywagen L,Makena N,Booley F,Tamgue O,Brombacher F

25-7133 was used in Flow cytometry/Cell sorting to identify cognitive changes during Nippostrongylus brasiliensis infection.

Tue Feb 13 00:00:00 EST 2018

Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells.

Arthritis research & therapy

Saigusa R,Asano Y,Taniguchi T,Hirabayashi M,Nakamura K,Miura S,Yamashita T,Takahashi T,Ichimura Y,Toyama T,Yoshizaki A,Trojanowska M,Sato S

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Wed Feb 07 00:00:00 EST 2018

HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.

Immunity

Osbourn M,Soares DC,Vacca F,Cohen ES,Scott IC,Gregory WF,Smyth DJ,Toivakka M,Kemter AM,le Bihan T,Wear M,Hoving D,Filbey KJ,Hewitson JP,Henderson H,Gonzàlez-Cìscar A,Errington C,Vermeren S,Astier AL,Wallace WA,Schwarze J,Ivens AC,Maizels RM,McSorley HJ

25-7133 was used in an ELISA assay to study how HpARI employs a novel molecular strategy to suppress type 2 immunity in both infection and allergy.

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Epidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion.

Immunity

Minutti CM,Drube S,Blair N,Schwartz C,McCrae JC,McKenzie AN,Kamradt T,Mokry M,Coffer PJ,Sibilia M,Sijts AJ,Fallon PG,Maizels RM,Zaiss DM

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

25-7133 was used in Flow cytometry/Cell sorting to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 EDT 2017

The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development.

Immunity

Miyazaki M,Miyazaki K,Chen K,Jin Y,Turner J,Moore AJ,Saito R,Yoshida K,Ogawa S,Rodewald HR,Lin YC,Kawamoto H,Murre C

25-7133 was used in Flow cytometry/Cell sorting to propose that the E-Id protein axis specifies innate and adaptive lymphoid cell fate.

Tue May 16 00:00:00 EDT 2017

Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region.

PloS one

Kim K,Kim N,Lee GR

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Thu Jul 07 00:00:00 EDT 2016

Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity.

Journal of immunology (Baltimore, Md. : 1950)

Contreras F,Prado C,González H,Franz D,Osorio-Barrios F,Osorio F,Ugalde V,Lopez E,Elgueta D,Figueroa A,Lladser A,Pacheco R

25713382 was used in flow cytometry to investigate how dopamine receptor D3 signaling affects the balance of effector T cells

Sun May 15 00:00:00 EDT 2016

Streptomycin treatment alters the intestinal microbiome, pulmonary T cell profile and airway hyperresponsiveness in a cystic fibrosis mouse model.

Scientific reports

Bazett M,Bergeron ME,Haston CK

25-7133 was used in Flow cytometry/Cell sorting to reveal a potential intestinal microbiome influence on lung response in BALB/c Cftr(tm1UNC) mice.

Tue Jan 12 00:00:00 EST 2016

Type 2 innate lymphoid cells control eosinophil homeostasis.

Nature

Nussbaum JC,Van Dyken SJ,von Moltke J,Cheng LE,Mohapatra A,Molofsky AB,Thornton EE,Krummel MF,Chawla A,Liang HE,Locksley RM

Published figure using IL-13 monoclonal antibody (Product # 25-7133-82) in Flow Cytometry

Thu Oct 10 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

Gene Aliases: Il-13; Il13

UniProt ID: (Mouse) P20109

Entrez Gene ID: (Mouse) 16163

Function(s)