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We offer a broad range of options and configurations for ordering custom DNA oligos. Review the information below to learn more about our minimum yield guarantees, delivery schedules, and resources for ordering.
To learn more about purification and modification options, visit our Oligo Modifications & Configurations page.
Our quality control processes help increase performance, speed, and capacity. The result is quick and efficient delivery. Below, find additional information about estimated yields based on length of oligo and starting synthesis scale, as well as minimum yields based on synthesize scale and purification option chosen.
When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmol amounts post-synthesis.
A universal characteristic of chemical synthesis of oligonucleotides is that the final yield is less than the starting synthesis scale. Despite coupling efficiencies greater than 99%, the iterative addition of each base in the oligo chain compounds the loss of potential full-length material you can achieve. The expected final yield is, therefore, dependent on the length of the oligo. In addition, some material loss also occurs during processing, purification, and quality control. For an example of how coupling efficiency affect final yield, see our FAQ on this topic.
Table 1 shows the estimated nmol yield for desalted oligos at each starting synthesis scale. This table converts the OD units into nmol values for an oligo with an equal and even distribution of each base. The actual nmol conversion from OD for a specific oligo may be different based on the composition and distribution of each base within the oligo.
| Oligo length | 25 nmol (2 OD) | 50 nmol (4 OD) | 200 nmol (16 OD) |
|---|---|---|---|
| 20 | 10 | 20 | 80 |
| 25 | 8 | 16 | 64 |
| 30 | 6.6 | 13.2 | 52.8 |
| 35 | 5.6 | 11.2 | 44.8 |
| 40 | 5 | 10 | 40 |
| 45 | 4.5 | 9 | 36 |
| 50 | 4 | 8 | 32 |
| 60 | 3.2 | 6.4 | 25.6 |
| 70 | 2.8 | 5.6 | 22.4 |
| 80 | 2.4 | 4.8 | 19.2 |
When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmol amounts post-synthesis.
Note: We guarantee the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.
Table 2 below shows minimum guarantee yields (OD260). Please note that OD guarantee is valid for unmodified oligos 20 to 100 bases in length.
Yields for oligos under 20 bases are not guaranteed. In general, they are about half of the values as shown in the table below.
| Bases | Scale | Desalted (OD) | Cartridge (OD) | HPLC (OD) | PAGE (OD) |
|---|---|---|---|---|---|
| >20mer | 25 nmol | 2 | NA | NA | NA |
| 50 nmol | 4 | 2 | 1 | 0.5 | |
| 200 nmol | 16 | 10 | 3 | 1 | |
| 1 µmol | 50 | 25 | 15 | 5 | |
| 10 µmol | 500 | NA | 150 | 50 |
Optional special handling may be added to your order. For account specific pricing, simply add your oligonucleotide item(s) to your cart and ensure you are logged in. Please note a handling charge of $12.50 per order will be applied to all oligo orders.
Free of charge special handling:
Special handling options for a fee:
For all inquiries, please email us.
Different modifications, scales, order sizes, oligo length, and purification choices will demand different lengths of synthesis time. For the most accurate ship date estimate, please enter your oligos in our online order tool. Approximate production time can be viewed in the tables below.
| Type of oligonucleotides | Ships within |
|---|---|
| Desalted, unmodified ≤40 bases ordered before 1 pm EST (U.S.) | Same day |
| Desalted, unmodified ≤40 bases ordered before 8 pm EST (U.S.) | 1 business day |
| Desalted 46–100 bases; phosphorylated oligonucleotides, cartridge purified and phosphorothioate oligonucleotides | 2 business days |
| 3′ & 5′ modified | 5 business days |
| HPLC and PAGE purity oligonucleotides; 1 µmole scale, 10 µmole scale |
For plate orders containing oligos of 50 bases or less.
| Number of plates | Ships within |
|---|---|
| 1–4 plates | 3 business days |
| 5–8 plates | 4 business days |
| 9–14 plates | 6 business days |
| 15–21 plates | 8 business days |
| 21–35 plates | 10 business days |
| 36 or more plates | Inquire |
For any 3′ or 5′ modifications, add 2 business days to each category.
For plate orders containing oligos greater than 50 bases in length, add 2 business days to each category.
What if there was a way to share your oligos order directly with your team, giving them the ability to manage and track shipments? Invitrogen Oligos now offers the ability to do just that. Order sharing—try it today!
For Research Use Only. Not for use in diagnostic procedures.