We offer a broad range of options and configurations for ordering custom DNA oligos. Review the information below to learn more about our minimimum yield guarantees; electronic codes to use for easy ordering by email or fax; pricing for custom DNA oligos and primers including synthesis scales, purification options and modifications; delivery schedules and resources for ordering.
To learn more about purification and modification options, visit our Oligo Configuration Options page.
Quality control and minimum yield guarantee
Our unique quality control processes help increase performance, speed, and capacity. The result is quick and efficient delivery. Below, find additional information about estimated yields based on length of oligo and starting synthesis scale, as well as minimum yields based on synthesize scale and purification option chosen.
When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmole amounts post-synthesis.
Note: We guarantee the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.
Key features of our unique processes
We use a state-of-the-art automated process to increase performance, speed and capacity. This means you get quality Custom Primers quickly and efficiently. Custom DNA Oligos are available as standard deoxynucleotides, modified based, 5′ modified nucleotides (including a wide variety of fluorescent dyes and enzyme conjugates), and S-oligos for antisense studies. Five scales and four purity levels are available for DNA oligos in both tube and plate formats.
Our unique process includes 100% in-process trityl monitoring with up to 12 measurements taken for every base addition and additional CE, MS, and OD to verify yield. This comprehensive QC also applies to our Next-Day delivery service. Our oligo offering is complimented by easy online ordering and overnight shipping for rapid, reliable delivery. Consistent oligonucleotide quality is guaranteed and a comprehensive Certificate of Analysis accompanies each order, indicating quality, molecular weight, extinction coefficient, sequence, and melting temperature. No other oligo vendor provides superior performance or better value.
Additional features of our process
- Final testing of statistically selected oligos by capillary electrophoresis or by mass spectroscopy to further ensure the quality.
- Certificate of Analysis includes oligo name; scale of synthesis; sequence and length; melting temperature (TM); amount in ODs, µg, and nmole; and molecular weight and extinction coefficient calculated from your exact sequence, including modification.
- Label information on each vial is durably printed on clear transparent stock to allow full view of contents.
- Fluorescent oligos shipped in amber tubes to protect light sensitive oligos.
- Award winning Technical Services for complete workflow support.
- Shipping in specially designed, easy-to-identify envelopes by express overnight delivery.
Synthesis scales refer to the amount of starting material present, not the amount of final product produced. This is the same for all manufacturers of synthetic DNA. When a 50 nmole scale synthesis is specified, approximately 50 nmoles of the first base is added to the DNA synthesizer. For an average 25-mer, at least 25% of this starting material will result in failure sequences, hence it is not possible to produce 50 nmoles of full-length product from a 50 nmole scale synthesis. In addition, further losses occur during processing, transfer of material, and quality control.
Estimated nmole yield
The table below shows estimated nmole yield for desalted oligos at the various starting synthesis scales. This table converts the OD units into nmole values for an oligo with an equal and even distribution of each base. The actual nmol conversion from OD for a specific oligo may be different based on the composition and distribution of each base within the oligo.
Approximate nmole ending yield at each starting synthesis scale
|Oligo length||25nmole (2 OD)||50nmole (4 OD)||200nmole (16 OD)|
When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmole amounts post-synthesis. Note: We guarantee the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.
The table below shows minimum guarantee yields (OD260). Please note that OD guarantee is valid for unmodified oligos 20 to 100 bases in length.
Yields for oligos under 20 bases are not guaranteed. In general they are about half of the values as shown in the table below.
Minimum yields for oligos of 20 bases or more by purification method
|Bases||Scale||Desalted (OD)||Cartridge (OD)||HPLC (OD)||PAGE (OD)|
For easy ordering using our Email or Fax methods, select the appropriate electronic codes provided here to specify your oligo format, synthesis scale and purification selection, sequence composition, and 3′- and 5′- modifications.
|Sequence and base modification||Code|
|Base, modification, or sequence||Code|
|Fluorescent Dye Modifications|
|Molecular Probes Dyes|
|Alexa Fluor 488||488|
|Alexa Fluor 532||532|
|Alexa Fluor 546||546|
|Alexa Fluor 555||555|
|Alexa Fluor 594||594|
|Alexa Fluor 647||647|
|Alexa Fluor 660||660|
|Alexa Fluor 750||750|
|Cascasde Blue Dye||CSB|
|Marina Blue Dye||MNB|
|Oregon Green 514||OGB|
|Oregon Green 488||OGC|
|Oregon Green 488-X||OGD|
|Pacific Blue Dye||PFB|
|Rhodamine Green Dye||RGA|
|Rhodol Green Dye||RGB|
We offer many options for custom DNA oligos and DNA primers, including all modification options as well as all synthesis scales. For account specific pricing, simply add your oligonucleotide item(s) to your cart. Review the tables below for more details on the following:
For pricing, please contact your local office or distributor. For next-day service, orders can only be placed online.
|Format||Synthesis scale||Purification||Size of order||Order cut-off time|
|Specifications||Tube||25 nmol||Desalted||1–20 oligos||1 p.m. EST|
|Next-day delivery charges|
|Next-Day Service fee per order ||$19.95 (USD)|
| Thermo Fisher Scientific will credit back the service fee if we are unable to ship the full order on time for next business day delivery. Please allow up to 1 week for the credit to be processed.|
Special handling features
|For plate orders||Price (USD)|
|Aliquoting, per oligo||$0.26|
|100% QC, per oligo||$0.25|
|Provide Remainders, per oligo||$0.30|
|Pooling, per oligo pair to be pooled||$0.25|
|Volume and concentration normalization||Free|
A shipping/handling charge of $12.50 per order will be applied. For all inquiries, please e-mail us.
Different modifications, scales, order sizes, oligo length and purification choices will demand different lengths of synthesis time. Check out our delivery schedules for your configuration needs.
Tubes delivery schedule
|Type of oligonucleotides||Ships within||Delivery on|
|Desalted, unmodified <=40 bases ordered before 1 pm EST (U.S.)||Same day||1st business day|
|Desalted, unmodified <=40 bases ordered before 8 pm EST (U.S.)|
|Desalted 41-100 bases; phosphorylated oligonucleotides, cartridge purified and phosphorothioate oligonucleotides||2 business days||3rd business day|
|3′ & 5′ modified||5 business days||6th business day|
|HPLC and PAGE purity oligonucleotides; 1 µmole scale, 10 µmole scale||7th business day|
Plate delivery schedule
For plate orders containing oligos of 50 bases or less.
|Number of plates||Ships within||Delivery on|
|1-4 plates||3 business days||4th business day|
|5-8 plates||4 business days||5th business day|
|9-14 plates||6 business days||7th business day|
|15-21 plates||8 business days||9th business day|
|21-35 plates||10 business days||11th business day|
|36 or more plates||Inquire|
For any 3′ or 5′ modifications, add 2 business days to each category.
For plate orders containing oligos greater than 50 bases in length, add 2 business days to each category.
- Standard overnight shipping
- Dried oligo – ambient shipping Monday through Saturday
- Resuspended oligo – frozen shipping Monday through Thursday
Oligos Ordering Tool now offers order sharing
What if there was a way to share your Oligos order directly with your team, giving them the ability to manage and track shipments? Invitrogen Oligos now offers the ability to do just that. Order sharing – try it today!
Primer design tools
For Research Use Only. Not for use in diagnostic procedures.