We offer a broad range of options and configurations for ordering custom DNA oligos. Review the information below to learn more about our minimum yield guarantees, delivery schedules, and resources for ordering.

To learn more about purification and modification options, visit our Oligo Modifications & Configurations page.

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Quality control and minimum yield guarantee

Our quality control processes help increase performance, speed, and capacity. The result is quick and efficient delivery. Below, find additional information about estimated yields based on length of oligo and starting synthesis scale, as well as minimum yields based on synthesize scale and purification option chosen.

When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmol amounts post-synthesis.

Note: We guarantee the total yield of oligonucleotide as a minimum number of nmol. Purification by HPLC, PAGE, and cartridge will also affect final product yield.

Estimated yields

A universal characteristic of chemical synthesis of oligonucleotides is that the final yield is less than the starting synthesis scale. Despite coupling efficiencies greater than 99%, the iterative addition of each base in the oligo chain compounds the loss of potential full-length material you can achieve. The expected final yield is, therefore, dependent on the length of the oligo. In addition, some material loss also occurs during processing, purification, and quality control. For an example of how coupling efficiency affect final yield, see our FAQ on this topic.

Table 1 shows the estimated nmol yield for desalted oligos at each starting synthesis scale. This table converts the OD units into nmol values for an oligo with an equal and even distribution of each base. The actual nmol conversion from OD for a specific oligo may be different based on the composition and distribution of each base within the oligo.

Table 1. Approximate nmol final yield for each starting synthesis scale

Oligo length25 nmole (2 OD)50 nmole (4 OD)200 nmole (16 OD)
20102080
2581664
306.613.252.8
355.611.244.8
4051040
454.5936
504832
603.26.425.6
702.85.622.4
802.44.819.2

Minimum yields

When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. The estimated yields above provide guidance on the amount of full-length material we typically achieve, but sequence, GC content, and other factors can vary the actual final yield. The calculator below provides the minimum yield we will ship for unmodified oligos of a given length, scale, and purification method. For modified oligos, please enter your oligo in the online oligo ordering portal to calculate minimum yields. Note: We guarantee the total yield of oligonucleotide as a minimum number of nmol.

Explore: Oligo minimum yield calculator


Pricing

For account specific pricing, simply add your oligonucleotide item(s) to your cart. Review the tables below for more details on the pricing for regular and next day service, and special handling features.

For pricing, please contact your local office or distributor. For next-day service, orders can only be placed online.

 Format
Synthesis scalePurification
Size of orderOrder cut-off time
SpecificationsTube25 nmolDesalted1–20 oligos1 p.m. EST
Price/base
(7-40)
$0.38 (USD)
Next-day delivery charges
Next-Day Service fee per order*$19.95 (USD)
*Thermo Fisher Scientific will credit back the service fee if we are unable to ship the full order on time for next business day delivery. Please allow up to one week for the credit to be processed.

Special handling features

For plate ordersPrice (USD)
Aliquoting, per oligo$0.26
Provide remainders, per oligo$0.30
Pooling, per oligo pair to be pooled$0.25
Volume normalizationFree
Concentration normalizationFree
Volume and concentration normalizationFree

Prices are included for your planning purposes only and may be subject to change. A shipping/handling charge of $12.50 per order will be applied. For all inquiries, please email us.


Delivery schedules

Different modifications, scales, order sizes, oligo length, and purification choices will demand different lengths of synthesis time. Check out our delivery schedules for your configuration needs.

Tubes delivery schedule

Type of oligonucleotidesShips withinDelivery on
Desalted, unmodified ≤40 bases ordered before 1 pm EST (U.S.)Same day1st business day
Desalted, unmodified ≤40 bases ordered before 8 pm EST (U.S.)1 business day2nd business day
Desalted 46–100 bases; phosphorylated oligonucleotides, cartridge purified and phosphorothioate oligonucleotides2 business days3rd business day
3′ & 5′ modified5 business days6th business day
HPLC and PAGE purity oligonucleotides; 1 µmole scale, 10 µmole scale 6 business days 7th business day


Plate delivery schedule

Tubes delivery schedule

Type of oligonucleotidesShips withinDelivery on
Desalted, unmodified <=40 bases ordered before 1 pm EST (U.S.)Same day1st business day
Desalted, unmodified <=40 bases ordered before 8 pm EST (U.S.) 1 business day 2nd business day
Desalted 41-100 bases; phosphorylated oligonucleotides, cartridge purified and phosphorothioate oligonucleotides2 business days3rd business day
3′ & 5′ modified5 business days6th business day
HPLC and PAGE purity oligonucleotides; 1 µmole scale, 10 µmole scale 6 business days 7th business day

Plate delivery schedule

For plate orders containing oligos of 50 bases or less.

Number of platesShips withinDelivery on
1-4 plates3 business days4th business day
5-8 plates4 business days5th business day
9-14 plates6 business days7th business day
15-21 plates8 business days9th business day
21-35 plates10 business days11th business day
36 or more platesInquire 

For any 3′ or 5′ modifications, add 2 business days to each category.
For plate orders containing oligos greater than 50 bases in length, add 2 business days to each category.

Domestic shipping:

  • Standard overnight shipping
  • Dried oligo – ambient shipping Monday through Saturday
  • Resuspended oligo – frozen shipping Monday through Thursday

Domestic shipping

  • Standard overnight shipping
  • Dried oligo—Ambient shipping Monday through Friday
  • Resuspended oligo—Frozen shipping Monday through Thursday or ambient shipping Monday through Friday
Oligos ordering tool now offers order sharing

What if there was a way to share your oligos order directly with your team, giving them the ability to manage and track shipments? Invitrogen Oligos now offers the ability to do just that. Order sharing—try it today!

For Research Use Only. Not for use in diagnostic procedures.