PEPotec Immuno Custom Peptide Libraries

Thermo Scientific PEPotec Immuno Peptide Libraries are fully synthetic custom libraries that support high-throughput screening assays to map epitopes or identify immunogenic sites in proteins. These libraries are fully customizable and contain an acetate counter ion to avoid potential toxicity in epitope discovery and mapping. These application-specific peptide libraries can be used in applications such as vaccine development, T- and B-cell research, antibody development, biomarker discovery, and kinase and protease studies.

PEPotec Immuno Peptide Library standard service

Length 6 to 20* amino acids; L-isoforms only
Manufacturing scale ≥0.1 mg
Purity options Crude (as synthesized)**
Formulation Lyophilized
Production time 2-3 weeks
Quality control*** Mass spectrometry (MS), 100% of samples
C-terminus residue Any unmodified L-isoform amino acid
Counterion Acetate
Delivery format Peptides are provided in individual 2-D barcoded tubes in 96-tube plates

* Please inquire about longer peptide lengths.
** No purification given, as peptide purity is sequence-dependent and a function of different liquid chromatographic conditions.
*** For all peptides except phosphopeptides, the three major peaks in the MS analysis represent the target of interest. Peptides must pass both the MS analysis and the final gross weight criteria (>1 mg) before shipment. If peptides do not pass these criteria, the customer will be informed and one resynthesis will be offered free of charge.

PEPotec immuno peptide libraries are fully customizable and supplied with acetate as the counterion to avoid potential toxicity issues in epitope discovery and mapping. These application-specific peptide libraries are ready to use in applications such as vaccine development, T and B cell research, antibody development, and biomarker discovery. Optional peptide modifications are available, including residue phosphorylation and acetylation, and different formulations are possible that enable study of cellular signaling events.

Highlights

  • Low toxicity—peptides with acetate as the counterion are less toxic in certain applications when compared to those with trifluoroacetate (TFA)
  • Application-specific—the 6- to 20–amino acid peptide length distribution fits most immunological applications
  • Convenient—peptides are provided in individual 2-D barcoded tubes in 96-tube plates, and each order includes a CD or flash-drive with the peptide sequences and positions in each plate
  • Flexible—optional services, including phosphorylation and acetylation, are available for studying signaling and regulatory proteins
PEPotec immuno peptide library optional services
Trifluoroacetate (TFA) counterion
Phosphorylation at 1-3 sites
Lys-Ac, internal
Peptides >20 and <26 amino acids in length
Delivered in 100% DMSO
Alphanumeric tube labelin

PEPotec Immuno Peptide optional modifications

  • C-terminal heavy labeling at R or K
  • Internal heavy labeling at A,R,I,L,K,F,P, or V
  • Alternative heavy amino acid at C-term
  • Alternative light amino acid at C-term
  • Phosphorylation at 1–3 sites
  • All cysteines protected by carbamidomethylation (CAM)
  • Diglycine ubiquitination motif on lysine [Lys(GG)]
  • Methionine sulfoxide [Met(O)]
  • Acetylation at side chain of lysine [Lys(Ac)]
  • Methylation at side chain of lysine [Lys(Me)] and arginine [Arg(Me)]
  • Dimethylation at side chain of lysine [Lys(Me)2]
  • 3-chloro-tyrosine
  • Hydroxyproline (Hyp)
  • Isoaspartic acid
  • Formylation at C-term
  • Carboxymethylcysteine (CMC) at cysteine

Frequently asked questions

What is the shelf life of a peptide?

A. The shelf life is >24 months at -20°C for lyophilized peptides protected by argon in the original non open container. This is sequence dependent and it may be shorter for some sequences.

Q. Why are PEPotec Immuno Libraries of higher quality than most competitors?

A. We are synthesizing our PEPotec using the solid phase Fmoc synthesis on resin. Most of our competitors use the spot technology providing poor quality peptides above 15 amino acids.

Q. How many peptide syntheses typically fail?

For peptide synthesis ~2.4% of the peptides submitted, due to difficult sequence were unable to be synthesized. Most of our competitors announce on their web site 5%.

Q. What peptide parameters are influenced by amino acid composition?

A. The ability to synthesize the peptide and its solubility, stability, and overall charge are discussed in the Protein Biology Learning Center under the Peptide Design.

Q. What information is needed to obtain an accurate quote?

A. All the information required to get an accurate quote is included in the Quote Request form.

PEPotec Immuno Peptide Library standard service

Length 6 to 20* amino acids; L-isoforms only
Manufacturing scale ≥0.1 mg
Purity options Crude (as synthesized)**
Formulation Lyophilized
Production time 2-3 weeks
Quality control*** Mass spectrometry (MS), 100% of samples
C-terminus residue Any unmodified L-isoform amino acid
Counterion Acetate
Delivery format Peptides are provided in individual 2-D barcoded tubes in 96-tube plates

* Please inquire about longer peptide lengths.
** No purification given, as peptide purity is sequence-dependent and a function of different liquid chromatographic conditions.
*** For all peptides except phosphopeptides, the three major peaks in the MS analysis represent the target of interest. Peptides must pass both the MS analysis and the final gross weight criteria (>1 mg) before shipment. If peptides do not pass these criteria, the customer will be informed and one resynthesis will be offered free of charge.

PEPotec immuno peptide libraries are fully customizable and supplied with acetate as the counterion to avoid potential toxicity issues in epitope discovery and mapping. These application-specific peptide libraries are ready to use in applications such as vaccine development, T and B cell research, antibody development, and biomarker discovery. Optional peptide modifications are available, including residue phosphorylation and acetylation, and different formulations are possible that enable study of cellular signaling events.

Highlights

  • Low toxicity—peptides with acetate as the counterion are less toxic in certain applications when compared to those with trifluoroacetate (TFA)
  • Application-specific—the 6- to 20–amino acid peptide length distribution fits most immunological applications
  • Convenient—peptides are provided in individual 2-D barcoded tubes in 96-tube plates, and each order includes a CD or flash-drive with the peptide sequences and positions in each plate
  • Flexible—optional services, including phosphorylation and acetylation, are available for studying signaling and regulatory proteins
PEPotec immuno peptide library optional services
Trifluoroacetate (TFA) counterion
Phosphorylation at 1-3 sites
Lys-Ac, internal
Peptides >20 and <26 amino acids in length
Delivered in 100% DMSO
Alphanumeric tube labelin

PEPotec Immuno Peptide optional modifications

  • C-terminal heavy labeling at R or K
  • Internal heavy labeling at A,R,I,L,K,F,P, or V
  • Alternative heavy amino acid at C-term
  • Alternative light amino acid at C-term
  • Phosphorylation at 1–3 sites
  • All cysteines protected by carbamidomethylation (CAM)
  • Diglycine ubiquitination motif on lysine [Lys(GG)]
  • Methionine sulfoxide [Met(O)]
  • Acetylation at side chain of lysine [Lys(Ac)]
  • Methylation at side chain of lysine [Lys(Me)] and arginine [Arg(Me)]
  • Dimethylation at side chain of lysine [Lys(Me)2]
  • 3-chloro-tyrosine
  • Hydroxyproline (Hyp)
  • Isoaspartic acid
  • Formylation at C-term
  • Carboxymethylcysteine (CMC) at cysteine

Frequently asked questions

What is the shelf life of a peptide?

A. The shelf life is >24 months at -20°C for lyophilized peptides protected by argon in the original non open container. This is sequence dependent and it may be shorter for some sequences.

Q. Why are PEPotec Immuno Libraries of higher quality than most competitors?

A. We are synthesizing our PEPotec using the solid phase Fmoc synthesis on resin. Most of our competitors use the spot technology providing poor quality peptides above 15 amino acids.

Q. How many peptide syntheses typically fail?

For peptide synthesis ~2.4% of the peptides submitted, due to difficult sequence were unable to be synthesized. Most of our competitors announce on their web site 5%.

Q. What peptide parameters are influenced by amino acid composition?

A. The ability to synthesize the peptide and its solubility, stability, and overall charge are discussed in the Protein Biology Learning Center under the Peptide Design.

Q. What information is needed to obtain an accurate quote?

A. All the information required to get an accurate quote is included in the Quote Request form.