HeavyPeptide AQUA Custom Synthesis Service

Thermo Scientific HeavyPeptide AQUA Custom Synthesis Service provides isotopically labeled, AQUA-grade peptides for the relative and absolute quantitation of proteins at very low concentrations in complex protein mixtures. Peptides are synthesized using the latest Fmoc solid-phase technology and purified by HPLC. All HeavyPeptide sequences come with mass spectrometric analysis and analytical HPLC data. Additionally, we offer a wide range of labels, modifications, scales, and purities.

HeavyPeptide AQUA Peptide specifications

Peptide lengthUp to 15* amino acids
Purity options95-97%
FormulationLyophilized or acetonitrile/H2O
Production time2-3 weeks AQUA Express, 5-7 weeks standard
ModificationsVariety of modifications and labels, including heavy amino acids (isotope-labeled) available
Delivery FormatGlass vial

* For longer peptide lengths and manufacturing scale please download the quote form from above and email the completed form to us

Available HeavyPeptide AQUA Peptide grades

 HeavyPeptide AQUA BasicHeavyPeptide AQUA QuantProHeavyPeptide AQUA Ultimate
Research StageDiscovery, confirmation, or validation, relative quantitationConfirmation or validation, ideal for biomarker verificationValidation or clinical, ideal for absolute quantitation
Standard (Light) versions availableYesYesYes
FormulationLyophilized5 pmol/µL in 5% (v/v) acetonitrile/H2O5 pmol/µL in 5% (v/v) acetonitrile/H2O
Concentration PrecisionNA±10-25%±5-10%
Peptide Purity>95%>97%>97%
Isotopic  Enrichment>99%>99%>99%
Peptide LengthUp to 15 amino acids†Up to 15 amino acids†Up to 15 amino acids†
Amount/aliquot number15-30nmol/1 aliquot10nmol/10 aliquots 40nmol/40 aliquots 96nmol/96 aliquots10nmol/10 aliquots 40nmol/40 aliquots 96nmol/96 aliquots
Quality ControlMSAnalytical HPLCQuantitative AAA
ShipmentDry at room temperatureIn solution on wet iceIn solution on wet ice
Delivery Time5-7 weeks standard AQUA, 2-3 weeks AQUA Express5-7 weeks standard AQUA, 2-3 weeks AQUA Express5-7 weeks standard AQUA, 2-3 weeks AQUA Express

† For longer peptide lengths please download the quote form from above and email the completed form to us

Product options

  • Additional light amino acids to extend the peptide length
  • Additional heavy amino acid on each peptide
  • Multiple solvents, concentrations and aliquot sizes available

HeavyPeptide AQUA Peptide optional heavy amino acids

Amino acid†CodeMass differenceIsotopeIsotopic enrichment
AlanineA+4 DaU-13C3, 15N>99%
ArginineR+10 DaU-13C6, 15N4>99%
IsoleucineI+7 DaU-13C6, 15N>99%
LeucineL+7 DaU-13C6, 15N>99%
LysineK+8 DaU-13C6, 15N2>99%
PhenylalanineF+10 DaU-13C9, 15N>99%
ProlineP+6 DaU-13C5, 15N>99%
ValineV+6 DaU-13C5, 15N>99%

† Other amino acids on request.

Peptide modifications

  • Single or double phosphorylation (pY, pT or pS)
  • Cysteine carbamidomethylation (CAM)
  • Chloro-L-Tyrosine
  • Pryoglutamic acid
  • Methionine oxidation (Met[O])
  • Other modifications available on request

‡ CAM tends to cause cyclisation at the N-terminus. Fully cyclized form can be provided upon request.

Frequently asked questions

Q. What is the shelf life of a HeavyPeptide AQUA?

A. The shelf life is >12 months at -20°C. For long term storage we recommend -80°C. A HeavyPeptide AQUA cannot be frozen again after first utilization without risking a dramatic change in the standard concentration. Usually an aliquot of HeavyPeptide AQUA standard can be kept a few days at 4°C without significant changes. The stability and accuracy of a HeavyPeptide AQUA may vary with the sequence.

Q. Do HeavyPeptides AQUA in solution degrade faster vs. lyophilized ones?

Lyophilized peptide are less prone to degradation, oxidation, reduction and other chemical modifications. However, it is usually not possible to reconstitute lyophilized peptides in a quantitative way as they tend to form colloids during the lyophilization process. This can result in unknown concentration of solubilized peptide, but one that can be measured using MS. In about 5% of the sequences less than 10% of the peptide is fully solubilized. This problem cannot be detected by the operator. For products provided fully solubilized (QuantPro & Ultimate) if a chemical modification happens it can be detected and the product discarded.

Q. How do you ship HeavyPeptides AQUA?

The Basic grade is shipped lyophilized protected with Argon in a moisture proof sealed bag. Each bag includes molecular sieve to maintain the relative humidity close to ~0%. The Basic grade is shipped at room temperature. The QuantPro and Ultimate grades are provided in solution and shipped on wet ice.

Q. What information is needed to obtain an accurate quote?

A. All the information required to get an accurate quote is included in the Quote Request form.

HeavyPeptide AQUA Peptide specifications

Peptide lengthUp to 15* amino acids
Purity options95-97%
FormulationLyophilized or acetonitrile/H2O
Production time2-3 weeks AQUA Express, 5-7 weeks standard
ModificationsVariety of modifications and labels, including heavy amino acids (isotope-labeled) available
Delivery FormatGlass vial

* For longer peptide lengths and manufacturing scale please download the quote form from above and email the completed form to us

Available HeavyPeptide AQUA Peptide grades

 HeavyPeptide AQUA BasicHeavyPeptide AQUA QuantProHeavyPeptide AQUA Ultimate
Research StageDiscovery, confirmation, or validation, relative quantitationConfirmation or validation, ideal for biomarker verificationValidation or clinical, ideal for absolute quantitation
Standard (Light) versions availableYesYesYes
FormulationLyophilized5 pmol/µL in 5% (v/v) acetonitrile/H2O5 pmol/µL in 5% (v/v) acetonitrile/H2O
Concentration PrecisionNA±10-25%±5-10%
Peptide Purity>95%>97%>97%
Isotopic  Enrichment>99%>99%>99%
Peptide LengthUp to 15 amino acids†Up to 15 amino acids†Up to 15 amino acids†
Amount/aliquot number15-30nmol/1 aliquot10nmol/10 aliquots 40nmol/40 aliquots 96nmol/96 aliquots10nmol/10 aliquots 40nmol/40 aliquots 96nmol/96 aliquots
Quality ControlMSAnalytical HPLCQuantitative AAA
ShipmentDry at room temperatureIn solution on wet iceIn solution on wet ice
Delivery Time5-7 weeks standard AQUA, 2-3 weeks AQUA Express5-7 weeks standard AQUA, 2-3 weeks AQUA Express5-7 weeks standard AQUA, 2-3 weeks AQUA Express

† For longer peptide lengths please download the quote form from above and email the completed form to us

Product options

  • Additional light amino acids to extend the peptide length
  • Additional heavy amino acid on each peptide
  • Multiple solvents, concentrations and aliquot sizes available

HeavyPeptide AQUA Peptide optional heavy amino acids

Amino acid†CodeMass differenceIsotopeIsotopic enrichment
AlanineA+4 DaU-13C3, 15N>99%
ArginineR+10 DaU-13C6, 15N4>99%
IsoleucineI+7 DaU-13C6, 15N>99%
LeucineL+7 DaU-13C6, 15N>99%
LysineK+8 DaU-13C6, 15N2>99%
PhenylalanineF+10 DaU-13C9, 15N>99%
ProlineP+6 DaU-13C5, 15N>99%
ValineV+6 DaU-13C5, 15N>99%

† Other amino acids on request.

Peptide modifications

  • Single or double phosphorylation (pY, pT or pS)
  • Cysteine carbamidomethylation (CAM)
  • Chloro-L-Tyrosine
  • Pryoglutamic acid
  • Methionine oxidation (Met[O])
  • Other modifications available on request

‡ CAM tends to cause cyclisation at the N-terminus. Fully cyclized form can be provided upon request.

Frequently asked questions

Q. What is the shelf life of a HeavyPeptide AQUA?

A. The shelf life is >12 months at -20°C. For long term storage we recommend -80°C. A HeavyPeptide AQUA cannot be frozen again after first utilization without risking a dramatic change in the standard concentration. Usually an aliquot of HeavyPeptide AQUA standard can be kept a few days at 4°C without significant changes. The stability and accuracy of a HeavyPeptide AQUA may vary with the sequence.

Q. Do HeavyPeptides AQUA in solution degrade faster vs. lyophilized ones?

Lyophilized peptide are less prone to degradation, oxidation, reduction and other chemical modifications. However, it is usually not possible to reconstitute lyophilized peptides in a quantitative way as they tend to form colloids during the lyophilization process. This can result in unknown concentration of solubilized peptide, but one that can be measured using MS. In about 5% of the sequences less than 10% of the peptide is fully solubilized. This problem cannot be detected by the operator. For products provided fully solubilized (QuantPro & Ultimate) if a chemical modification happens it can be detected and the product discarded.

Q. How do you ship HeavyPeptides AQUA?

The Basic grade is shipped lyophilized protected with Argon in a moisture proof sealed bag. Each bag includes molecular sieve to maintain the relative humidity close to ~0%. The Basic grade is shipped at room temperature. The QuantPro and Ultimate grades are provided in solution and shipped on wet ice.

Q. What information is needed to obtain an accurate quote?

A. All the information required to get an accurate quote is included in the Quote Request form.