We offer a broad range of options and configurations for ordering custom DNA oligos. Review the information below to learn more about our minimimum yield guarantees; electronic codes to use for easy ordering by email or fax; pricing for custom DNA oligos and primers including synthesis scales, purification options and modifications; delivery schedules and resources for ordering.  

To learn more about purification and modification options, visit our Oligo Configuration Options page.


Quality control and minimum yield guarantee

Our unique quality control processes help increase performance, speed, and capacity. The result is quick and efficient delivery. Below, find additional information about estimated yields based on length of oligo and starting synthesis scale, as well as minimum yields based on synthesize scale and purification option chosen. 

When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmole amounts post-synthesis. Note: We guarantee the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.

Key features of our unique processes

We use a state-of-the-art automated process to increase performance, speed and capacity. This means you get quality Custom Primers quickly and efficiently. Custom DNA Oligos are available as standard deoxynucleotides, modified based, 5′ modified nucleotides (including a wide variety of fluorescent dyes and enzyme conjugates), and S-oligos for antisense studies. Five scales and four purity levels are available for DNA oligos in both tube and plate formats.

Our unique process includes 100% in-process trityl monitoring with up to 12 measurements taken for every base addition and additional CE, MS, and OD to verify yield. This comprehensive QC also applies to our Next-Day delivery service. Our oligo offering is complimented by easy online ordering and overnight shipping for rapid, reliable delivery. Consistent oligonucleotide quality is guaranteed and a comprehensive Certificate of Analysis accompanies each order, indicating quality, molecular weight, extinction coefficient, sequence, and melting temperature. No other oligo vendor provides superior performance or better value.

Additional features of our process

  • Final testing of statistically selected oligos by capillary electrophoresis or by mass spectroscopy to further ensure the quality.
  • Certificate of Analysis includes oligo name; scale of synthesis; sequence and length; melting temperature (TM); amount in ODs, µg, and nmole; and molecular weight and extinction coefficient calculated from your exact sequence, including modification.
  • Label information on each vial is durably printed on clear transparent stock to allow full view of contents.
  • Fluorescent oligos shipped in amber tubes to protect light sensitive oligos. 
  • Award winning Technical Services for complete workflow support.
  • Shipping in specially designed, easy-to-identify envelopes by express overnight delivery.

Estimated yields

Synthesis scales refer to the amount of starting material present, not the amount of final product produced. This is the same for all manufacturers of synthetic DNA. When a 50 nmole scale synthesis is specified, approximately 50 nmoles of the first base is added to the DNA synthesizer. For an average 25-mer, at least 25% of this starting material will result in failure sequences, hence it is not possible to produce 50 nmoles of full-length product from a 50 nmole scale synthesis. In addition, further losses occur during processing, transfer of material, and quality control.

Estimated nmole yield 

The table below shows estimated nmole yield for desalted oligos at the various starting synthesis scales. This table converts the OD units into nmole values for an oligo with an equal and even distribution of each base. The actual nmol conversion from OD for a specific oligo may be different based on the composition and distribution of each base within the oligo.

Approximate nmole ending yield at each starting synthesis scale

Oligo length 25nmole (2 OD) 50nmole (4 OD) 200nmole (16 OD)
20 10 20 80
25 8 16 64
30 6.6 13.2 52.8
35 5.6 11.2 44.8
40 5 10 40
45 4.5 9 36
50 4 8 32
60 3.2 6.4 25.6
70 2.8 5.6 22.4
80 2.4 4.8 19.2

 

Minimum yields

When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmole amounts post-synthesis. Note: We guarantee the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.

The table below shows minimum guarantee yields (OD260). Please note that OD guarantee is valid for unmodified oligos 20 to 100 bases in length.

Yields for oligos under 20 bases are not guaranteed. In general they are about half of the values as shown in the table below.

Minimum yields for oligos of 20 bases or more by purification method

Bases Scale Desalted (OD) Cartridge  (OD) HPLC (OD) PAGE (OD)
>20mer 25 nmole 2 NA NA NA
50 nmole 4 2 1 0.5
200 nmole 16 10 3 1
1 µmole 50 25 15 5
10 µmole 500 NA 150 50

Electronic codes

For easy ordering using our Email or Fax methods, select the appropriate electronic codes provided here to specify your oligo format, synthesis scale and purification selection, sequence composition, and 3′- and 5′- modifications.

Format Code
Tubes T
Plates P
Synthesis scale Code
25 nmoles 25N
50 nmoles 50N
200 nmoles 200N
1 µmole 1U
10 µmoles 10U
Purification options Code
Desalted DSL
Cartridge COP
HPLC HPL
PAGE PAG
Sequence and base modification Code
Deoxyadenine A
Deoxycytosine C
Deoxyguanidine G
Thymidine T
Deoxyuracil U
Deoxyinosine I
A-Phosphorothioate F
C-Phosphorothioate O
G-Phosphorothioate E
T-Phosphorothioate Z
A+C+G V
A+C+G+T N
A+T+G D
T+C+G B
A+T+C H
A+T W
C+G S
T+G K
A+C M
C+T Y
A+G R
Base, modification, or sequence Code
Deoxyadenine A
Deoxycytosine C
Deoxyguanidine G
Thymidine T
Deoxyuracil U
Deoxyinosine I
A-Phosphorothioate F
C-Phosphorothioate O
G-Phosphorothioate E
T-Phosphorothioate Z
A+C+G V
A+C+G+T N
A+T+G D
T+C+G B
A+T+C H
A+T W
C+G S
T+G K
A+C M
C+T Y
A+G R

3′ Modification Code
Amine® AMN
Biotin BIO
Phosphate PHO
5′ Modification Code
General Modifications
Aldehyde ALD
Alkaline Phosphatase ALP
Amine® (Primary) AMN
Biotin BIO
Phosphate PHO
Thiol THL
Gateway Forward GWF
Gateway Reverse GWR
Fluorescent Dye Modifications
FAM FAM
Fluorescein FLO
HEX HEX
ROX ROX
TET TET
TAMRA TAM
Molecular Probes Dyes
Alexa Fluor 488 488
Alexa Fluor 532 532
Alexa Fluor 546 546
Alexa Fluor 555 555
Alexa Fluor 594 594
Alexa Fluor 647 647
Alexa Fluor 660 660
Alexa Fluor 750 750
BODIPY FL BDA
BODIPY 530/550 BDB
BODIPY 493/503 BDC
BODIPY 558/569 BDE
BODIPY 564/570 BDF
BODIPY 576/589 BDG
BODIPY 581/591 BDH
BODIPY FL-X BDI
BODIPY TR-X BDJ
BODIPY TMR BDK
BODIPY R6G BDL
BODIPY R6G-X BDM
BODIPY 630/650 BDN
BODIPY 650/665 BDP
Cascasde Blue Dye CSB
Marina Blue Dye MNB
Oregon Green 514 OGB
Oregon Green 488 OGC
Oregon Green 488-X OGD
Pacific Blue Dye PFB
Rhodamine Green Dye RGA
Rhodol Green Dye RGB
Rhodamine Green-X RGC
Rhodamine RED-X RRA
Texas Red TXR
Texas Red-X TRX

Pricing

We offer many options for custom DNA oligos and DNA primers, including all modification options as well as all synthesis scales. For account specific pricing, simply add your oligonucleotide item(s) to your cart. Review the tables below for more details on the following:

For pricing, please contact your local office or distributor. For next-day service, orders can only be placed online.

  Format
Synthesis scale Purification
Size of order Order cut-off time
Specifications Tube 25 nmol Desalted 1–20 oligos 1 p.m. EST
Price/base
(7-40)
$0.38 (USD)
Next-day delivery charges
Next-Day Service fee per order [1] $19.95 (USD)
[1] Thermo Fisher Scientific will credit back the service fee if we are unable to ship the full order on time for next business day delivery. Please allow up to 1 week for the credit to be processed.

Special handling features

For plate orders Price (USD)
Aliquoting, per oligo $0.26
100% QC, per oligo $0.25
Provide Remainders, per oligo $0.30
Pooling, per oligo pair to be pooled $0.25
Volume normalization Free
Concentration normalization Free
Volume and concentration normalization Free

A shipping/handling charge of $12.50 per order will be applied. For all inquiries, please e-mail us.


Delivery schedules

Different modifications, scales, order sizes, oligo length and purification choices will demand different lengths of synthesis time. Check out our delivery schedules for your configuration needs.

Tubes delivery schedule

Type of oligonucleotides Ships within Delivery on
Desalted, unmodified <=45 bases ordered before 5 pm EST (U.S.) 1 business day 2nd business day
Desalted 46-100 bases; phosphorylated oligonucleotides, cartridge purified and phosphorothioate oligonucleotides 2 business days 3rd business day
3′ & 5′ modified 5 business days 6th business day
HPLC and PAGE purity oligonucleotides; 1 µmole scale, 10 µmole scale 6 business days 7th business day

Plate delivery schedule

For plate orders containing oligos of 50 bases or less.

Number of plates Ships within Delivery on
1-4 plates 3 business days 4th business day
5-8 plates 4 business days 5th business day
9-14 plates 6 business days 7th business day
15-21 plates 8 business days 9th business day
21-35 plates 10 business days 11th business day
36 or more plates Inquire  

For any 3′ or 5′ modifications, add 2 business days to each category.
For plate orders containing oligos greater than 50 bases in length, add 2 business days to each category.

Domestic shipping:

  • Standard overnight shipping
  • Dried oligo – ambient shipping Monday through Saturday
  • Resuspended oligo – frozen shipping Monday through Thursday

Resources

Primer design tools

Ordering details