Minimum Yield Guarantee

Synthesis scales refer to the amount of starting material present, not the amount of final product produced. This is the same for all manufacturers of synthetic DNA. When a 50 nmole scale synthesis is specified, approximately 50 nmoles of the first base is added to the DNA synthesizer. For an average 25-mer, at least 25% of this starting material will result in failure sequences, hence it is not possible to produce 50 nmoles of full-length product from a 50 nmole scale synthesis. In addition, further losses occur during processing, transfer of material, and quality control.

Estimated nmole Yield
The table below shows estimated nmole yield for desalted oligos at the various starting synthesis scales. This table converts the OD units into nmole values for an oligo with an equal and even distribution of each base. The actual nmol conversion from OD for a specific oligo may be different based on the composition and distribution of each base within the oligo.

 

 

Approximate nmole Ending Yield at Each Starting Synthesis Scale

Oligo Length 25nmole (2OD) 50nmole (4OD) 200nmole (16OD)
20 10 20 80
25 8 16 64
30 6.6 13.2 52.8
35 5.6 11.2 44.8
40 5 10 40
45 4.5 9 36
50 4 8 32
60 3.2 6.4 25.6
70 2.8 5.6 22.4
80 2.4 4.8 19.2

Minimum Yields
When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonecleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmole amounts post-synthesis. Invitrogen guarantees the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.

The table below shows minimum guarantee yields (OD260). Please note that OD guarantee is valid for unmodified oligos 20 to 100 bases in length.

Yields for oligos under 20 bases are not guaranteed. In general they are about half of the values as shown in the table below.

BASES SCALE DESALTED (OD) CARTRIDGE (OD) HPLC (OD) PAGE (OD)
>20mer 25 nmole 2 n/a n/a n/a
50 nmole 4 2 1 0.5
200 nmole 16 10 3 1
1 µmole 50 25 15 5
10 µmole 500 n/a 150 50

 

 


Electronic Codes

Format Code
Tubes T
Plates P

 

Synthesis Scale Code
50 nmoles 50N
200 nmoles 200N
1 µmole 1U

 

Purification Options Code
Desalted DSL
Cartridge COP
HPLC HPL
PAGE PAG

 

Modification Code
Deoxyadenine A
Deoxycytosine C
Deoxyguanidine G
Thymidine T
Deoxyuracil U
Deoxyinosine I
A-Phosphorothioate F
C-Phosphorothioate O
G-Phosphorothioate E
T-Phosphorothioate Z
A+C+G V
A+C+G+T N
A+T+G D
T+C+G B
A+T+C H
A+T W
C+G S
T+G K
A+C M
C+T Y
A+G R

 

3’ Modification Code
Amine® AMN
Biotin BIO
Phosphate PHO
Thiol THL

 

5’ Modification Code
General Modifications
Aldehyde ALD
Amine® (Primary) AMN
Biotin BIO
Phosphate PHO
Thiol THL
Fluorescent Dye Modifications
FAM FAM
Fluorescein FLO
HEX HEX
ROX ROX
TET TET
TAMRA TAM
Molecular Probes™ Dyes
Alexa Fluor 488 488
Alexa Fluor 532 532
Alexa Fluor 546 546
Alexa Fluor 555 555
Alexa Fluor 594 594
Alexa Fluor 647 647
Alexa Fluor 660 660
Alexa Fluor 750 750
BODIPY® FL BDA
BODIPY® 530/550 BDB
BODIPY® 493/503 BDC
BODIPY® 558/569 BDE
BODIPY® 564/570 BDF
BODIPY® 576/589 BDG
BODIPY® 581/591 BDH
BODIPY® FL-X BDI
BODIPY® TR-X BDJ
BODIPY® TMR BDK
BODIPY® R6G BDL
BODIPY® R6G-X BDM
BODIPY® 630/650 BDN
BODIPY® 650/665 BDP
CASCADE BLUE™ Dye CSB
MARINA BLUE™ Dye MNB
OREGON GREEN® 514 OGB
OREGON GREEN® 488 OGC
OREGON GREEN® 488-X OGD
PACIFIC BLUE™ Dye PFB
RHODAMINE GREEN™ Dye RGA
RHODOL GREEN™ Dye RGB
RHODAMINE GREEN™-X RGC
RHODAMINE RED™-X RRA
TEXAS RED® TXR
TEXAS RED®-X TRX

 


Pricing for Oligonucleotides

Rough Price of Oligonucleotides

Synthesis
Scale
Price
per base
Length
Purification Modification
Price
50 nmol 60JPY 20 mer Desalted
(+0JPY)
None (+0JPY) 1,200JPY
50 nmol 60JPY
20 mer Cartridge
(+800JPY)
None (+0JPY)
2,000JPY
50 nmol 60JPY
30 mer Cartridge
(+800JPY)
None (+0JPY)
8,800JPY
50 nmol 60JPY
30 mer HPLC
(+7,000JPY)
5’ Phosphate
(+8,000JPY)
16,800JPY

Please click here for information of delivery time.

Standard Price List (*Free shipping on All Orders)

Synthesis
Scale *1
Price
per base
Desalted Cartridge HPLC *2 PAGE *3
50 nmol 60JPY 0JPY 800JPY 7,000JPY 7,500JPY
200 nmol 120JPY 0JPY 800JPY 7,000JPY 10,000JPY
1 μmol 500JPY 0JPY 3,000JPY 12,000JPY 12,000JPY
  1. “Synthesis scale” means the starting material volume. Final delivery material volume will change by base number and sequence. Please click here for minimum guarantee volume.
  2. HPLC can provide up to 65mer.
  3. Delivery time is 3weeks because performing in US site.

Value Oligo

Synthesis Scale *1 Price per base
Desalted   
40 nmol *2 1000JPY
  1. “Synthesis scale” means the starting material volume.
  2. Minimum guarantee volume is same as 50n mol scale's one.
  3. 7-30mer, you can include mix oligonucleotides.
  4. Value oligo can provide non modification and desalted.

Oligonucleotides(Plate)

Synthesis
Scale
Price
per base
Purification Length Delivery
Formulation
50 nmol 40JPY Desalted
7 - 80 mer TE Solution
(100uM)
200 nmol 80JPY Desalted
7 - 80 mer TE Solution
(500uM)
  • Please contact to order support if you want to order more than 81mer or 11plates.
  • Invitrogen can provide desalted or cartridge puritication for plate oligonucleotides.
  • Please contact to order support if you want to order HPLC or PAGE purification for plate oligonucleotides.
  • You can order the greater than or equal 24 primers.

Dilution buffer : TE, (Option) Sterilized water, etc. Shipping plate format : Deep well plate, (Option) Half well plate or Micro well plate. Quality Control : Trityl monitoring, Capillary electrophoresis, Mass analysis, Absorbance determination(OD260).

Price List for Modifications

Modification

50 nmol

200 nmol

1 μmol

5'-Amine*1, *2 6,000JPY/oligo 8,000JPY/oligo 10,000JPY/oligo
5'-Thiol - 14,000JPY/oligo 23,000JPY/oligo
5'-Phosphate*2 8,000JPY/oligo 10,000JPY/oligo 12,000JPY/oligo
5'-Biotin*1, *2 8,000JPY/oligo 10,000JPY/oligo 15,000JPY/oligo
5'-HEX, TET, 6-FAM
10,000JPY/oligo 12,000JPY/oligo 15,000JPY/oligo
5'-Fluorescein
8,000JPY/oligo 10,000JPY/oligo 15,000JPY/oligo
5'-ROX*6 - 13,000JPY/oligo 29,000JPY/oligo
5'-TAMRA*6 - 13,000JPY/oligo 29,000JPY/oligo
5'-Texas Red*6
5'-Texas Red-X*6
- 7,000JPY/oligo 30,000JPY/oligo
5'-Alexa*6
350、405、430、488、514、532、546、555、568、594、633、647、660、680、700、750
10,000JPY/oligo 13,000JPY/oligo 18,000JPY/oligo
3'-Amine*1,*4 5,000JPY/oligo 8,000JPY/oligo -
3'-Phosphate*4 7,000JPY/oligo 10,000JPY/oligo 12,000JPY/oligo
3'-Thiol*3,*6 - 14,000JPY/oligo -
3'-Biotin*1,*4 8,000JPY/oligo 10,000JPY/oligo 18,000JPY/oligo
Deoxyinosine*5 1,800JPY/modified base 1,800JPY/modified base 2,500JPY/modified base
Deoxyuracil*5 3,000JPY/modified base 3,000JPY/modified base 4,000JPY/modified base
Phosphorothioates(S-oligos)*5 700JPY/modified base 1,000JPY/modified base 1,600JPY/modified base
Mixed Bases Free
  1. We are doing sale/synthesis under license from Wakunaga Pharmaceutical Co., Ltd.
  2. We cannot take cartridge purification order.
  3. If you order HPLC purification, you can choose the protecting group of terminal thiol.
    - Protection group "ON" (-SSR. do not treat deprotection reaction.)
    - Protection group "OFF" (-SH. treat deprotection reaction.)
    If you order desalted or cartridge purification, we can ship only protecting group “ON”.
  4. If you request cartridge purification, we can provide up to 55mer.
  5. The price of one base is including standard price and additional price.
    ex) 50nmol, Desalted, Deoxyinosine => 60JPY + 1,800JPY = 1,860JPY/modified base
  6. We can provide HPLC purification only.
  7. We cannot provide the modification of phosphorothioates on 3’terminal by structure issue of oligonucleotide.
    Please choose oligonucleotide the A or G or C or T on 3' terminal.
    If you request phosphorothioates modification on 5’ terminal, please contact to customer service.

<Order code for phosphorothioate>

 

Oxidized base

A

C

G

T

Phosphorothioate base

F

O

E

Z

If you want to request other modification, please contact to customer service.

Delivery schedule

Synthesis Scale

Purification

7~50mer

 51~80mer

81~100mer

50 nmol

Desalted

1 2 3

Cartridge

1 2

HPLC*1

5 5
(Up to 65mer)
200 nmol

Desalted

1* 2 3

Cartridge

1 2 4

HPLC*1

5 5
(Up to 65mer)
1 μmol

Desalted

4 4 4

Cartridge

4 4 4

HPLC*1

5 5
(Up to 65mer)

(Business Day)

Value Oligo

Purification

7~30mer

40nmol Desalted 1

(Business Day)

  1. Delivery schedule is subject to change.
  2. This delivery schedule is applied to the order by Web(-20:00), E-mail(-16:00), FAX(-14:00).

For all inquiries, please contact us.
Life Technologies Japan Ltd. 
Order support (Custom primer)
TEL:03-6832-6980
FAX:03-6832-9585
E-mail:jpprimerorder@invitrogen.com


Delivery Schedules for Oligonucleotides

Easy to spot, oligonucleotides are shipped in specially marked envelopes. Rapid, reliable delivery is ensured by overnight express. The table below highlights specific service and delivery time frames. Delivery schedules may be affected by demand fluctuations. We make every effort to deliver your high quality oligonucleotides as quickly as possible.

 

Tubes Delivery Schedule

Type of Oligonucleotides (50nmole scale) Ships within Delivery on
Desalted unmodified
<=50 mer ordered before 4 p.m.
1 business day 1 business day
Desalted unmodified
51-80 mer order before 8 p.m.
2 business days 2 business day
3’ & 5’modified 2 business days - 3 business day -
HPLC purity oligonucleotides 4 business days -
5 business day -

* Delivery time will change on delivery area.
* Delivery time is subject to change based on ordered oligos number and modification type.
* If you have any futher questions or concerns, please feel free to contact to customer service.

Plate Delivery Schedule

For plate orders containing disalted unmodified oligos of 50 mer or less.

Number of Plates Ships Within Delivery on
(50nmol scale) -10 plates 14 business days 15 business days
(50nmol scale) 11- plates inquire  
(200nmol scale) -10 plates 14 business days 15 business days
(200nmol scale) 11- plates inquire  

* If you have any futher questions or concerns, please feel free to contact to customer service.


Ordering Details


Oligo Quality Control

Key Features of our Unique Processes

Invitrogen uses a state of the art automated process to increase performance, speed and capacity. This means you get quality Custom Primers quickly and efficiently. Custom DNA Oligos are available as standard deoxynucleotides, modified based, 5’ modified nucleotides (including wide variety of fluorescent dyes, enzyme conjugates), and S-oligos for antisense studies. Five scales and four purity levels are available for DNA oligos in both tube and plate formats.

Our unique process includes 100% in-process trityl monitoring with up to 12 measurements taken for every base addition and additional CE, MS, and OD to verify yield. This comprehensive QC also applies to our Next-Day delivery service. Our oligo offering is complimented by easy online ordering and overnight shipping for rapid, reliable delivery. Consistent oligonucleotide quality is guaranteed and a comprehensive Certificate of Analysis accompanies each order, indicating quality, molecular weight, extinction coefficient, sequence, and melting temperature. No other oligo vendor provides superior performance or better value.

Additional Features of our Process

  • Final testing of statistically selected oligos by capillary electrophoresis or by mass spectroscopy to further ensure the quality.
  • Certificate of Analysis includes oligo name; scale of synthesis; sequence and length; melting temperature (TM); amount in ODs, µg, and nmole; and molecular weight and extinction coefficient calculated from your exact sequence, including modification.
  • Label information on each vial is durably printed on clear transparent stock to allow full view of contents.
  • Fluorescent oligos shipped in amber tubes to protect light sensitive oligos. Award winning Technical Services for complete workflow support.
  • Shipping in specially designed, easy-to-identify envelopes by express overnight delivery.

品質へのこだわり

研究者の皆様から常に期待されるのは、卓越性、信頼性、選択性です。ライフテクノロジーズでは、高純度・高品質のオリゴDNA合成サービスをご提供しております。また、品質マネジメントシステム規格「ISO9001」の認証を取得し、より一層の顧客満足度の向上を実現すべく、製品の高品質化への取り組みを継続しております。

 

BSI認証取得 FM 562152 / ISO9001:2008

信頼性の高い品質管理

一連のシステムで合成:正確・効率的・エラーを最小限に抑制しています。
カスタムプライマーの合成工程は特許取得済みの単一のソフトウェアシステムにより作動されています。注文から分析書作成、配送までの精度と効率が保証されています。

 

 

Invitrogen独自の合成機PASシステム

Invitrogen独自のParallel Array Synthesis (PAS) システムにより、96ウェルプレート上で96種類のカスタムプライマーを並行して合成できます。先進的なflow-throughプロセスにより交差汚染とハンドリングエラーのリスクを抑制しており、その結果、高品質で正確な合成が保証されています。

 

 

独自のシステム(特許取得済み)により高品質を確保

特許取得済みのソフトウェアとPASシステム;エラー率を抑制
革新的な脱保護システム;製品の品質を向上
独自の空気ろ過乾燥システム;配送条件が安定し、プライマーの溶解が迅速かつ容易になります。

合成前、合成中、合成後に行うQC管理

すべてのサプライヤーと原料を厳格に検証しています。
PASは製造工程QCを利用しています;合成工程を通じて各カスタムプライマーをモニターしています。
合成後にPASによって統計的サンプリングが行われたサンプルをマス・スペクトロメトリーやキャピラリー電気泳動(CE)により分析する事によって、合成されたオリゴDNAの品質が確保されています。

出荷について

出荷基準値に満たないプライマーは全て再合成を行い、品質検査を通過後に出荷いたします。再合成時には、品質検査を通過したプライマーのみ先行してお届けしますので、同時にご注文いただいても別便で届く場合がございますのでご了承ください。もし、同時発送をご希望の場合には、再合成へ入らせていただく旨のご連絡を差し上げる時にご連絡ください。