Vanquish UHPLC Systems

Developed without compromise

Thermo Scientific™ Vanquish™ systems were designed from the ground up to deliver a new standard in UHPLC — more results with better separations, without compromise. These easy-to-use, fully integrated and biocompatible systems feature high sample capacity for high-throughput workflows, industry-leading pumping performance, exceptionally high S/N and linearity, two-mode thermostatting, and both active and passive pre-heating preheating in a system that's driven by the gold-standard CDS software.

Featured Vanquish UHPLC systems

For any application—whether HPLC or UHPLC, small or large molecules, simple or complex mixtures—the Vanquish platform was designed to handle it all. This innovative, simple-to-operate, and easy-to-maintain platform delivers confident separations that push chromatographic boundaries. Regardless of the configuration you choose, you will get a highly integrated solution with optimal fluidic connections, providing unsurpassed retention time and peak area precision.

You will also benefit from the industry-leading, easy-to-use Thermo Scientific Chromeleon Chromatography Data System (CDS) software with eWorkflow method setup, dedicated ePanels, and smart tools. Chromeleon CDS software delivers advanced system communication with single-point intelligent control and functionality. It also provides direct plugins for the Vanquish system to other manufacturers' chromatography data systems.

Choose from two Vanquish UHPLC systems:

Vanquish UHPLC System – High Performance UHPLC

Designed for extra high performance and throughput, the high-end, state-of-the-art Vanquish UHPLC system was conceived for the uniquely efficient, smaller 1.5 µm solid core particle column. The system handles samples with more accuracy, controls separations with more power and greater confidence, detects analytes with more sensitivity, and features easy maintenance with tool-free connections.

Vanquish Flex UHPLC System –  Built for Biopharma

Built on our award winning Vanquish UHPLC platform, the Vanquish Flex system is designed for maximum flexibility to meet all of the necessary applications and method development for the biopharmaceutical market and any other development workflows.. This biocompatible UHPLC system includes state-of-the-art quaternary solvent blending for method development and application flexibility. The Vanquish Flex system is ideal for:

  • Aggregates
  • Fragments
  • Variants
  • Glycoforms
  • Glycan analysis
  • Peptide maps
  • Post-translational modifications (PTMs)
  • Oxidations
  • Oligonucleotides
  • Drug antibody ratios (DARs)

Vanquish UHPLC system configurations and options

Vanquish Horizon
UHPLC System

Vanquish Flex
Binary UHPLC System

Vanquish Flex
Quaternary UHPLC System

    Most advanced UHPLC with revolutionary flow delivery High pressure binary solvent blending for fast, reliable UHPLC Quaternary solvent blending for flexible method development


System pressure
Up to 1500 bar

Up to 1000 bar

Up to 1000 bar
Retention time precision
Flow range
5 mL/min

8 mL/min

8 mL/min

System Features

Design Modular Modular Modular
SmartInject technology for best retention time precision      
SmartFlow technology for excellent flow precision      
Pump High-pressure gradient proportioning High-pressure gradient proportioning Low-pressure gradient formation
Active solvent
Multi-mode column thermostatting      
Sample cooling      
Viper fittings for virtually zero dead volume system connections      
    Thermo Scientific Viper UHPLC Fittings Kits
Order all the UHPLC fittings and capillaries you need for your system or application in a single kit

System components

Pump Vanquish Binary Pump H
The pump with the highest backpressure at 1500 bar; ideal for shallow gradient performance
Vanquish Binary Pump F
UHPLC performance for demanding routine applications up to 1000 bar
Vanquish Quaternary Pump F
UHPLC performance with more solvent flexibility up to 1000 bar
Autosampler Vanquish Split Sampler HT
Excellent precision, accuracy, and short cycle times with innovative air-stream cooling and barcode reading for inventory management for all workflows up to 1500 bar
Vanquish Split Sampler FT
Enjoy the same benefits as the Vanquish Split Sampler HT for applications up to 1000 bar
Column compartment Vanquish Column Compartment H
Provides two column thermostatting modes (still and forced air), active and passive solvent pre-heating and post column cooling for optimized control of your separations and advanced method transfer.
Extended sample capacity Vanquish Charger
An intelligent, robotic rack and plate feeder that automatically loads samples in the autosampler and extends the sample capacity of all Vanquish samplers to up to 8832 samples (23 wellplates or 12 vial racks) with innovative air-stream cooling and barcode reading for inventory management.

Compatible components for all Vanquish UHPLC systems

Detector options Vanquish columns Chromeleon CDS software

Take advantage of the system's extended pressure capabilities and robustness by using columns that were developed for Vanquish, allowing you to separate your science better, faster, and easier than ever before.

Vanquish can be used with any of our HPLC and UHPLC columns

  • Streamlines your data processing
  • Industry-leading ease of use and intelligent functionality
  • Unique user guidance and hardware automation features designed for Vanquish systems

Interact with Vanquish

Take a virtual tour of the Vanquish Horizon, and see how each module's advanced technology will separate your science from the status quo.

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Featured Vanquish UHPLC system videos