Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).
Features of SMPH:
• Reactive groups: NHS ester and maleimide • Reactive towards: amino and sulfhydryl groups • Slightly longer and more soluble than SMPB • NHS ester reacts with primary amines at pH 7-9, forming a stable amide bond • Maleimide reacts with -SH groups at pH 6.5-7.5, forming a stable thioether bond • Non-cleavable • Water-insoluble (dissolve first in DMF or DMSO)
Product References: Crosslinker Application Guide -- search for recent literature references for this product