PNPP Substrates
PNPP Substrates
Thermo Scientific™

PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
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Catalog NumberDescription
34045PNPP Powder
34047PNPP Tablets
37620PNPP Substrate Kit
376211-Step™ PNPP Substrate Solution
4 Options
Catalog number 34045
Price (USD)/ 25 g
192.00
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Add to cart
Description:
PNPP Powder
Request bulk or custom format
Price (USD)/ 25 g
192.00
Add to cart
PNPP Substrates
Catalog number34045
Price (USD)/ 25 g
192.00
-
Add to cart
PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative

The substrate is offered in several formats:
PNPP Substrate
• Powder Form

PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance

PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.

1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPNPP Powder
Product TypeELISA Substrate
Quantity25 g
Detection MethodColorimetric
FormPowder
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Shipping ConditionAmbient
Unit Size25 g
Contents & Storage
Upon receipt store product at 4°C.