Thermo Scientific™

PNPP Substrate

Catalog number:  34045

Related applications:

Protein Assays and Analysis

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Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Features of PNPP (p-nitrophenyl phosphate):

• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative

PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. The substrate is offered in several formats.

Related Products
1-Step™ PNPP Substrate Solution
Pierce™ PNPP Substrate Kit
PNPP Substrate Tablets
For Research Use Only. Not for use in diagnostic procedures.


Substrate Properties: Chemical Substrate
Detection Method: Colorimetric
Substrate Type: AP (Alkaline Phosphatase) Substrate
Description: Pierce PNPP Powder

Contents & storage

Upon receipt store product at 4°C. Product is shipped at ambient temperature.