Invitrogen™

TrueTag™ Donor DNA Kit, DDK

Catalog number: A53808
Invitrogen™

TrueTag™ Donor DNA Kit, DDK

Catalog number: A53808
Catalog Number
A53808
Unit Size
10 reactions
Price (USD)
Price: 694.00
Online price: 690.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A5380810 reactions
Price: 694.00
Online price: 690.65
Your price:
-
Product Overview
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The Invitrogen TrueTag Donor DNA Kit, DDK, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

Benefits of the TrueTag Donor DNA kits include:
• One-step PCR amplification to generate donor templates—no cloning required
• Generate transfection-ready DNA donors in hours instead of days
• Enrich up to 100% DDK-tagged cells

The TrueTag Donor DNA Kit, DDK, provides everything you need to produce a high-quality DNA donor to tag your gene with a selection marker and DDK—simply provide two locus-specific primers to add homology arms, PCR-amplify, and purify for a transfection-ready donor in hours, not days. Each kit is designed to enable you to:
• Tag the carboxy- (C-) or amino- (N-) terminus of your gene of interest
• Choose from puromycin or blasticidin selection markers—whatever best suits your cell model
• Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment

Included in the kit
• Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers to tag human beta-actin (ACTB)

TrueDesign Genome Editor software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper knock-in of your fluorescent tag. Identify your target gene transcript of interest and the TrueDesign Genome Editor web-based software does the work for you:
1. Displays your target gene transcript
2. Designates your insertion site
3. Designs and enables easy ordering of all required components not included in the TrueTag kit:
    • CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
    • Oligos to PCR-amplify your donor and add appropriate homology arms
    • Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
    • Verification primers to validate proper insertion of the TrueTag DNA Donor

Also required and available for purchase separately or as part of TrueDesign workflow:
TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneArt CRISPR mRNA
CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent

For Research Use Only. Not for use in diagnostic procedures

Specifications

For Use With (Application)
Protein Detection and Isolation
No. of Reactions
10 reactions

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