Células transportadoras de SLC (G) simuladas

TRANSiPORT human SLC transporter cells are HEK293 cells that transiently overexpress a solute carrier (SLC) transporter protein. Solute carriers are one of two superfamilies of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes into and out of cells. Membrane transporters are found in hepatic cells, kidney proximal tubule cells, intestinal cells, and brain cells.

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Yes, the protocol is available here (https://www.genomembrane.com/information).

Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of SLC transport due to the presence of endogenous transporters or non-specific binding.

Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

One vial contains 8-12 million cells with high viability, designed to seed one 24-well plate for single use.