Thermo Scientific™

Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Catalog number: PG82073
Thermo Scientific™

Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Catalog number: PG82073
Catalog Number
PG82073
Unit Size
25 g
Price (USD)
Price: 508.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
1 g
Price (USD)
Price: 108.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PG8207325 g
Price: 508.00
Your Price:
-
PG820791 g
Price: 108.00
Your Price:
-
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Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Features of Premium Grade EDC:

EDC—carbodiimide crosslinker for conjugation between carboxylates and amines
High quality—identity and purity confirmed by several tests
Product integrity—enhanced level of testing and characterization compared to standard grade
Lot retention—ample supply of past lots retained to ensure future process testing
Change management—Change Control Notification (CCN) service
Consistent manufacture—batch-specific manufacturing documentation review

Compared to the standard grade product, Premium Grade EDC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. EDC is 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride, a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation. In the presence of N-hydroxysulfosuccinimide (sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding sulfo-NHS.

Specifications of Premium Grade EDC
We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
• Visual: White crystalline powder, free of foreign matter
• Identity: IR scan shows only peaks characteristic of EDC
• Water content: Karl Fischer titration, ≤ 2%
• Trace metals: All metals, including lead, ≤ 50 ppm
• Elemental analysis: Reported values for C, H, and N

Applications:
• Conjugate carboxyl and amino groups among peptides and proteins
• Couple haptens to immunogenic carrier proteins (e.g., attach a peptide to KLH)
Immobilize peptide antigens to affinity-purify antibodies
• Create NHS-activated, amine-reactive labeling compounds
• Crosslink proteins to carboxyl coated beads or surfaces
• Activate nanoparticles with amine-reactive sulfo-NHS esters

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity
Amine-Carboxyl
Cleavable
No
Description
EDC
Molecular Weight
191.7
PEGylated
No
Spacer Arm Length
0.0 Å
Cell-Permeable
No
Shipping Condition
Wet Ice
Product Line
Pierce™
Labeling Method
Chemical Labeling
Crosslinker Type
Heterobifunctional
Reactive Moiety
Carbodiimide
Spacer
Short (<10 Å)
Form
Powder
Quantity
25 g
Solubility
Water
Format
Standard, Single use, Premium grade
Water-Soluble
Yes
Product Type
Crosslinker

Contents & Storage

Upon receipt store desiccated at -20°C.