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Exceed in biopharma, metabolomics, proteomics, and structural biology applications
Make groundbreaking discoveries with the Thermo Scientific Orbitrap Excedion Pro Mass Spectrometer, which sets a new standard in analytical performance.
Biopharmaceutical: Versatility for characterizing different molecular domains
The Orbitrap Excedion Pro BioPharma Mass Spectrometer ensures comprehensive characterization by detecting and quantifying impurities and modifications, performing accurate quantitative analysis, and enhancing top-down, middle-down, and bottom-up capabilities. It streamlines workflows for rapid, high-throughput analysis, significantly reducing time-to-results and increasing productivity.
UHPLC-HRAM-MS-ddMS2 analysis of NISTmAb tryptic digest peptide mapping experiment, EThcD unambiguously identified the two leucine residues (loss of 43Da from z9 and z12 fragments) and one isoleucine residue (loss of 29Da from z6 fragments) in this 13-amino acids doubly charged peptide with complete sequence coverage. Zoom-in insets show high mass accuracy (<5ppm) achieved for the signature fragments.
Signature fragments (c+57 and z-57) generated by ETD distinguish isoaspartic acid from aspartic acid.b: In a UHPLC-HRAM-MS-ddMS2 analysis of NISTmAb tryptic digest peptide mapping experiment, EThcD achieved high sequence coverage and unambiguously identified isoaspartic acid in a 23-amino acid triply charged deamidated peptide present at a low abundance level (0.18%) with high confidence. The zoom-in insets showed the high mass accuracy (<8 ppm) achieved for both signature fragments (z6-57 and c17+57).
What customers are saying about the value to their biotechnology work
"The Orbitrap Exploris 480 MS has been indispensable for biotherapeutic characterization, running continuously in our lab and producing high-quality data. The Orbitrap Excedion Pro Biopharma MS enhances this platform through the addition of electron transfer dissociation (EASY-ETD) and an extended mass range. Initial data shows that high spectral acquisition rate of EASY-ETD results in deeper protein sequence coverage, improved PTM characterization, detection of large aggregate impurities, and richer fragmentation patterns for confident disulfide linkage assignments. These improvements provide immediate value to our biotechnology work and will serve as our next generation platform."
Dr. Andrew Mahan
Associate Director and Mass Spec Group Leader
Johnson & Johnson Innovative Medicine
Structural biology: A novel workflow for higher order structure characterization
The Orbitrap Excedion Pro Biopharma Mass Spectrometer-based HDX MS offers a comprehensive and novel tool set for the analysis of protein conformation, conformation dynamics, and protein-protein interactions:
- High sensitivity and scan speed enable in-depth coverage for Data-dependent acquisition (DDA) and Data-independent acquisition (DIA) workflows using Hydrogen deuterium exchange mass spectrometry (HDX-MS)
- 100% sequence coverage with 7.5 redundancy for HC and 5.2 for LC of monoclonal antibodies or antibody-drug conjugates can be achieved in 6 min with loads as low as 300 ng
Peptide identification and sequence coverage of Rituximab heavy chain (HC) and light chain (LC) across different on-column injection amounts (300 ng, 700 ng, and 1.5 µg) using a 6-minute gradient. The bar graph represents the number of identified peptides for HC (dark blue) and LC (light blue), while the orange line indicates sequence coverage percentage.
Optimized DIA workflow for high-throughput HDX-MS analysis
The fast scan speed of the Orbitrap Excedion Pro Biopharma Mass Spectrometer enables precise and high-throughput HDX-MS analysis, facilitating accurate protein-ligand binding screening.
Identification of binding pocket on KRAS G12C upon Adagrasib binding using DIA-HDX. (a) Woods plot. X-axis denotes the residue number, while y-axis shows percentage change in deuteration. Blue bars with error bars indicate experimental data points at 20sec labeling time, representing regions with significant deuteration changes. Dashed lines indicate a threshold for significant changes in deuteration. (b) Structural representation of KRAS G12C in complex with Adagrasib. The protein backbone is depicted in gray, with the G12C mutation region and key structural elements highlighted in blue.
What customers are saying about HDX-workflows
"The Orbitrap Exploris 480 MS has been essential for our high-throughput HDX-MS workflows, consistently delivering reproducible and reliable peptide-level deuterium uptake data. The Orbitrap Excedion Pro Biopharma mass spectrometer further enhances this capability with exceptional sensitivity, optimized low-flow setup, and advanced data-independent acquisition (DIA) strategies. Our initial evaluation demonstrated high-quality peptide-level resolution and improved detection of low-abundance peptides, enabling more comprehensive and precise characterization of protein-ligand and protein-protein interactions. These advancements make the Orbitrap Excedion Pro Biopharma MS an invaluable platform for next-generation HDX-MS studies."
Dr. Malvina Papanastasiou
Group Leader, Research Scientist, Broad Institute of MIT and Harvard
Metabolomics: Advanced instrument for sensitive analysis
The Orbitrap Excedion Pro Mass Spectrometer is an essential tool for metabolomics research, providing numerous advantages that enhance the analytical capabilities for comprehensive metabolite profiling and targeted metabolite analysis.
Untargeted discovery with eDR to unlock deeper insights
NIST SRM 1950 plasma metabolite extracts, separated using reversed phase and interrogated with iterative data dependent MS2 analysis using AcquireX deep scan workflow. Shown: 2X the number of compounds detected with MS1. Here, a compound refers to a unique m/z and RT-pair, after background subtraction, with isotopes, adducts and in-source fragments coalesced into a single entry. After injections, over 21% more compounds were annotated using mzCloud mass spectral library. Full scan MS1 to generate the precursor inclusion list for the data dependent workflow done with eDR workflow or Orbitrap full scan mode without eDR and results processed using Compound Discoverer 3.4 software.
Improved dynamic range of detected compounds with eDR
When plotting a regression of peak area versus total number of compounds detected in SRM 1950, the eDR scan mode achieves an additional order of magnitude of depth for plasma metabolite identification. This indicates that the greater than 2X gain in compounds are on the low end of the dynamic range without compromising the high-end measurements.
Improved sensitivity of low abundant compounds with enhanced dynamic range (eDR)
Annotated compound found in plasma only when using eDR scan mode. The mass spectrum on the bottom right indicates that A0 and A1 peaks are measured and can be used to further confidently confirm the molecular formula of the species as C19H40N3O9P [H]+.
The Orbitrap Excedion Pro MS delivers superior clarity: minimizing in-source fragmentation
Full MS spectrum of Phenylalanine in plasma with only 5% of the precursor being fragmented.
What customers are saying about quantitative accuracy for metabolic research
"Mass spectrometers with extended dynamic range offer a significant advantage by enabling the simultaneous analysis of both trace-level and highly abundant analytes without signal saturation or the loss of low-intensity peaks. This perspective will benefit the analysis of biological, environmental, and industrial samples with a broad range of analyte concentrations. By improving isotopic and adduct distribution analysis and enhancing detection limits, such advanced mass spectrometers can significantly improve quantitative accuracy, making them indispensable for high-precision analytical applications."
Dr. Oliver Fiehn
Director, West Coast Metabolomics Center, UC Davis Genome Center
Proteomics: New capabilities for targeted and discovery proteomics
The Orbitrap Excedion Pro MS transforms everyday proteomics into exceptional results.
- Accurate quantification with extended dynamic range
- Hybrid-DIA, Parallel reaction monitoring (PRM) and DIA in a single experiment
- Post-translational modifications analysis with EThcD fragmentation
- Immunopeptidomics with EThcD fragmentation
- Deep proteome coverage
Sequence coverage of all Peptide spectrum matches (PSMs) when using either HCD fragmentation or EThcD fragmentation. Sequence coverage was calculated by matching theoretical fragments with 10 ppm mass accuracy and confirming the charge state.
Sample: Immunopeptides from Jurkat cells, courtesy of Dr. Albert Heck.
What customers are saying about error-prone immunopetidomics research
"EThcD fragmentation on the Orbitrap Excedion Pro Mass Spectrometer boosts peptide sequence coverage, minimizing detrimental sequence gaps, even for peptides with unfavorable physicochemical properties, allowing less error-prone immunopeptidomics, glycoproteomics and de novo sequencing."
Dr. Albert J.R. Heck
Professor of Pharmaceutical Sciences, Biomolecular Mass Spectrometry and Proteomics
Utrecht University, The Netherlands
For Research Use Only. Not for use in diagnostic procedures.