Using as little as 2 ng of FFPE-derived DNA, the new Applied Biosystems TrueMark MSI Assay has a panel of 13 microsatellite markers to test for MSI associated with Lynch syndrome-related cancers beyond colorectal cancer and studying immunotherapy. The included analysis and reporting software featuring automated calling can help you have a more efficient workflow.
Microsatellite instability (MSI) is the condition of genetic hypermutability that results from impaired DNA mismatch repair (MMR). Studies indicate that the presence of high levels of MSI (MSI-H) might be predictive of Lynch syndrome, a hereditary condition associated with increased cancer risk across a variety of tumor types, including colorectal, gastric, and endometrial cancers.
Defects in MMR also result in novel somatic mutations in unrelated loci throughout the genome. These mutations can produce immunogenic proteins that can “prime” an immune response to the foreign neoantigens in the tumor. MSI-H tumors are potentially more sensitive to immune checkpoint inhibitor treatment than microsatellite-stable (MSS) tumors, making MSI analysis across diverse tumors important for the identification of those whose growth might be slowed by immunotherapy treatments.
The TrueMark MSI Assay is a low-input, affordable assay with an expanded 13-MSI-marker panel and includes TrueMark MSI Analysis Software. The entire workflow takes as little as four hours to determine sample status. The TrueMark MSI Assay leverages a 6-dye set to incorporate the additional markers and does not require the use of a tumor-normal match, cutting the number of wells required per sample in half and reducing sample handling burden.
In this recorded webinar, our speakers discuss how genotypic biomarkers are being used to better understand cancer biology, develop prognostic markers, and predict therapeutic success.
Included at no additional cost, TrueMark MSI Analysis Software is designed specifically for the TrueMark MSI Assay to enable automated calling of markers and samples, offering greater ease of analysis by reducing the manual QC, analysis, and review required to determine sample status.
For Research Use Only. Not for use in diagnostic procedures.