See this collection of resources to help you learn more about HILIC columns, their use and application.
HILIC Retention Mechanisms
Evidence suggests that the retention mechanism in HILIC is complex, and consists of a combination of:
- Hydrophilic partitioning of the analyte between the aqueous-rich layer and the bulk of the mobile phase
- Hydrogen bonding between polar functional groups and the stationary phase
- Electrostatic interactions on ionized functional groups
Additionally, van der Waals interactions between the hydrophobic portions of the bonded ligands of the stationary phase and the non-polar part of the analytes may be present.