Hydrophilic Interaction Liquid Chromatography (HILIC) columns

HILIC is one of the most successful approaches for the retention and separation of polar compounds. There are many stationary phases that can be used in HILIC mode, and many phases are generically described as HILIC independently of their chemistry.  There is also a variety of misinformation regarding the use of this technique. This information center will address many the questions that HILIC users face:

  • What column should be used?
  • What are the best mobile phase starting conditions?
  • What are the common issues in HILIC method development that need to be addressed

HILIC Stationary Phases

Understanding the possible HILIC retention mechanisms involved for a particular column-solvent-analyte system is crucial in the process of selecting the right HILIC stationary phase leading to a successful application.

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