Simplify the complexity for carbohydrate analysis
Automatically generate eluent gradients to analyze from simple to complex carbohydrates by operating your Thermo Scientific Dionex ICS-6000 HPIC system in Dual Eluent Generation Cartridge (Dual EGC) mode. The EGC cartridges in series with each other to generate the extremely reproducible and accurate KOH/KMSA eluent gradient to resolve mono, di- and oligosaccharides, as well as sialic acids.
The Dual EGC mode is a powerful Thermo Scientific Dionex "Just Add Water" electrolytic eluent generation technology. Making manual eluent preparation unnecessary, electrolytic eluent generation is a key feature of our Reagent-Free Ion Chromatography (RFIC) systems equipped with Thermo Scientific Chromeleon CDS software.
The 1 mm format Thermo Scientific CarboPac columns for Dual EGC provide predictable, high-resolution separations of monosaccharides, disaccharides, sialic acids, and complex oligosaccharides.
The columns are:
- Thermo Scientific Dionex CarboPac PA200 1 mm
- Thermo Scientific Dionex CarboPac PA20 1 mm
- Thermo Scientific Dionex CarboPac PA1 1 mm
Browse our complete portfolio below, or let one of our experts help you tailor a selection specific to your application and workflow needs.
Related Products For Automated Carbohydrate Analysis
The Dual EGC Mode Cost Savings Calculator helps you estimate your yearly savings when running your Thermo Scientific Dionex ICS-6000 HPIC system in Dual EGC mode rather than preparing eluents manually.
Why use Dual EGC for carbohydrate analysis?
Dual EGC for carbohydrate analysis provides faster, easier start-up, cleaner eluents, less maintenance, and greater reproducibility.
|Eliminates manual preparation of eluents|
|Improves IC reproducibility|
|Maximizes instrument uptime, minimizes maintenance|
|Offers eluent gradient capabilities|
|Eliminates sodium acetate issues|
Ion chromatography system configuration in Dual EGC mode
Dual EGC mode is available on the Dionex ICS-6000 HPIC system to support complex carbohydrate analysis. In Dual EGC mode, this RFIC-enabled system employs methanesulfonic acid (MSA) and potassium hydroxide (KOH) EGC cartridges, in series, to electrolytically generate potassium hydroxide/potassium methanesulfonate (KOH/KMSA) eluents. This operating mode is applicable to both capillary (0.4mm) and analytical (1.0mm) column formats.
For analytical (1mm format) operation — The Thermo Scientific Dionex EGC 400 (KOH and MSA) Eluent Generator Cartridges are designed to enable operation at flow rates between 20µL/min–200µL/min. The Dionex EGC 400 MSA cartridge is connected in series to a Dionex EGC 400 KOH cartridge to generate KOH/KMSA eluents up to a total concentration of 200mM at flow rates of 20–63µL/min, and up to 63mM at flow rates up to 200µL/min.
For capillary (0.4mm format) operation — A Dionex EGC-MSA (Capillary) Eluent Generator Cartridge is connected in series to a Dionex EGC-KOH (Capillary) Eluent Generator Cartridge to generate KOH/KMSA eluents up to a total concentration of 200mM at flow rates of 1–10µL/min, and up to 100mM at flow rates up to 20µL/min.
See the difference in your oligosaccharide results
Data comparison: manual eluent preparation vs. Dual EGC mode eluent preparation (1mm and 0.4mm format)
This informational web page provides answers to:
This informational web page:
- Discusses how high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) can simplify carbohydrate analysis
- Provides links to major application notes for HPAE-PAD carbohydrate analysis