Viper Fittings

Fingertight zero-dead volume HPLC fittings and kits

Thermo Scientific Viper and nanoViper HPLC fittings, connectors, tubing, and kits allow you to quickly connect HPLC modules, valves, and columns for leak-free connections that improve chromatographic results by minimizing dead volume. Viper and nanoViper fittings systems and kits require no tools for installation, fit narrow connections (e.g., 10-port valves), and combine simplicity with high performance.

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We offer the following HPLC fittings, tubing, connections, and workflow kits:

  • Viper fingertight fitting systems, which are intended for HPLC and UHPLC and seal at the tip of the tubing and virtually eliminate dead volume, making it easier to plumb your HPLC or UHPLC system without any tools for reproducible leak-free connections to pressures up to 1500 bar. The Viper Fingertight fittings are reusable and available as stainless steel (SST) UHPLC fittings or as biocompatible PEEK HPLC fittings and alloy MP35N UHPLC fittings.
  • nanoViper fingertight fittings systems, which are fused silica UHPLC fittings - Avoid data-corrupting dead volumes in the capillary connections of your nano HPLC systems. The nanoViper Fingertight fitting system are reusable and consist of PEEK-shielded fused silica tubing and fingertight seal fittings.
  • UHPLC and HPLC connection kits with Viper fingertight fittings for Vanquish UHPLC systems and UltiMate 3000 HPLC systems, which contain all the HPLC connectors and capillaries needed for running standard or advanced workflows with your HPLC and LC-MS systems.
  • Application kits with nanoLC fittings, which contain all the necessary zero dead volume fittings and capillaries for connecting your UltiMate 3000 RSLCnano system to Thermo Scientific Orbitrap or triple quadrupole mass spectrometer depending on your nanoLC application requirement.

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Achieve better HPLC and UHPLC connections with Viper and nanoViper Fingertight fittings

Viper and nanoViper Fingertight Fittings are designed for the entire fluidic pathway in all HPLC/UHPLC systems for improved chromatography results
For more details on the various elements of this system, please read the list below.
Fingertight

1) Fingertight: Easy installation and fingertight up to 1500 bar.

Removable knurl

2) Removable knurl: Easy to grip and easy to remove, taking up less space to fit the tightest gaps.

ID color code

3) ID color code: Easy visualization with color-coded tags for quick ID recognition.

Identification tag

4) Identification tag: Quickly locate important information such as part number, material, length and ID dimensions.

Robustness

5) Robustness: withstands high temperatures up to 120 °C and is available in biocompatible materials.

More options

6) More options: A variety of materials and dimensions (length and ID) available for nearly every system and application.

Universal connections

7) Universal connections: Compatible with most columns and valves and small enough to fit in the most confined spaces, such as on 10-port valves.

Virtually zero dead volume

8) Virtually zero dead volume: Seals at the tip of the tubing to minimize extra column volumes for higher efficiency and better chromatography–no more tailing peaks.


Conventional vs Viper fittings

Conventional fittings often create extra-column volumes by incorrect positioning of the ferrule or by the capillary slipping through the ferrule when subjected to high pressures. The chromatogram demonstrates deteriorated peak shape caused by a slipped capillary at a backpressure of only 600 bar (8700 psi).

Viper fingertight fittings does not use a ferrule and minimizes extra-column volume by design. The chromatogram overlay shows consistent peak shapes under identical conditions to those used with conventional fitting in the figure above.


Featured Viper fingertight fittings

Check which HPLC connections fit your HPLC, UHPLC, or nano LC systems

HPLC and UHPLC fittings

Our HPLC and UHPLC fittings seal at the tip of the tubing, virtually eliminating dead volumes and making it easier to plumb your HPLC or UHPLC system without any tools for reproducible leak-free connections on pressures up to 1500 bar.

Our HPLC and UHPLC fittings, connections, tubing, crosses, tees, and kits are reusable and available in stainless steel (SST), PEEK and a biocompatible alloy (MP35N). The nanoViper HPLC fittings consist of PEEK-shielded fused silica tubing and a fingertight sealing fitting. Find specific fittings and capillaries or purchase complete solution kits for your nano LC system or applications.


Overview of Viper fingertight fitting system

  Viper
Stainless Steel
Viper
PEEK
Viper
MP35N
nanoViper

Connection principle

Fingertight fitting

Tubing type

Flexible
stainless steel
(SST)
PEEK
Biocompatible
MP35N
Biocompatible
PEEK-shielded fused silica

Wetted material

PEEK, SST PEEK PEEK, MP35N PEEK, fused silica

Maximum pressure

1310 bar 345 bar 1500 bar 1200 bar

Maximum temperature

120°C 30°C 120°C 120°C

Viper nut threading

Compatible with 10-32 threaded ports

Outer diameter

0.79 mm (1/32")

Inner diameter (ID)

100 µm (0.004")
130 µm (0.005")
180 µm (0.007")
65 µm (0.0025")
90 µm (0.0035")
130 µm (0.005")
100 µm (0.004")
130 µm (0.005")
180 µm (0.007")
10 µm (0.0004")
20 µm (0.0008")
50 µm (0.0020")
75 µm (0.0030")
100 µm (0.004")
150 µm (0.006")

Available lengths

65 mm to 950 mm 65 mm to 850 mm 65 mm to 950 mm 70 mm to 1100 mm
*customizable lengths

Fittings kits

Order all of the fittings and capillaries for your application in a single kit. These kits contain all the components to effortlessly plumb your system from start to finish.

Analytical flow HPLC/UHPLC Application kits

nanoLC Application kits

= Not applicable = Poor = Good = Better = Best

HPLC fittings, HPLC connectors, and workflow kits

HPLC fittings for HPLC and UHPLC systems

Stainless steel fittings in a range of lengths and interior diameters. Compatible with virtually any common column and valve type, and suitable for system pressures of up to 1310 bar (19,000 psi).

Length (mm) 100 µm ID 0.004 in. ID 130 µm ID 0.005 in. ID 180 µm ID 0.007 in. ID
65 6040.2207 6040.2307 6040.2357
100 6040.2322
150 6040.2215 6040.2315 6040.2360
200 6040.2327
250 6040.2225 6040.2325 6040.2385
350 6040.2235 6040.2335 6040.2375
450 6040.2245 6040.2345 6040.2365
550 6040.2255 6040.2305 6040.2355
650 6040.2265 6040.2310 6040.2395
750 6040.2320 6040.2370
850 6040.2330 6040.2380
950 6040.2340 6040.2390
PEEK HPLC fittings and MP35N UHPLC fittings

These HPLC and UHPLC fittings are available in a variety of lengths and diameters and are biocompatible.

Length (mm) 65 µm ID 0.0025 in. ID PEEK 90 µm ID 0.0035 in ID PEEK 130 µm ID 0.005 in. ID PEEK 100 µm ID 0.004 in. ID MP35N 130 µm ID 0.005 in. ID MP35N 180 µm ID 0.007 in. ID MP35N
65 6041.9075 (L 75 mm) 6042.2306
150 6041.5615 6041.5616 6042.2320 6042.2315
250 6041.5625 6041.9025 6042.2330 6042.2327
350 6042.2340 6042.2337
450 6042.2350 6042.2365
550 6042.2360 6042.2355
650 6041.5665 6042.2370 6042.2380
750 6041.5675 6042.2390 6042.2373 6042.2375
850 6041.5685
950 6042.2395 6042.2385
PEEK-shielded fused silica UHPLC fittings

These nano-, capillary-, and micro-flow UHPLC fittings are designed to overcome the drawbacks of conventional fittings to ensure perfect and easy connections for maximum performance and robustness with low-flow LC.

Length (mm) 10 µm ID 0.0004 in. ID 20 µm ID 0.0008 in. ID 50 µm ID 0.002 in. ID 75 µm ID 0.003 in. ID 100 µm ID 0.004 in. ID 150 µm ID 0.006 in. ID
70 6041.5120 6041.5123 6041.5126 6041.5810 6041.5817
150 6041.5118 (L 180 mm) 6041.5121 6041.5124 6041.5127 6041.5811 6041.5818
250 6041.5730 6041.5812 6041.5819
350 6041.5240 6041.5540 6041.5735 6041.5813 6041.5820
450 6041.5814 6041.5821
550 6041.5260 6041.5560 6041.5760 6041.5815 6041.5822
650 6041.5275 6041.5575 6041.5775 6041.5824
750 6041.5280 6041.5580 6041.5780 6041.5816 6041.5823
850 6041.5785
950 6041.5122 6041.5125 6041.5128
1100 6041.5711 6041.5828
Viper MS connection kits for Vanquish and UltiMate LC systems

Viper application kits

For easy setup dedicated Viper application kits contain all the capillaries needed for advanced analytical LC applications:

  • Automated Method Scouting
  • Online SPE
  • Tandem LC
  • And more!

Avoid data-destroying dead volumes when connecting your LC and LC-MS systems. Find all your necessary HPLC capillary connections, tubing, and fittings here with our comprehensive workflow kits for different HPLC and UHPLC systems. These kits contain all the connectors and capillaries you need and ensures consistently perfect connections. Please note that special instrument configurations are required for certain kits.

Description UltiMate 3000 SD system UltiMate 3000 RS system Vanquish (Duo) HPLC systems
Viper Capillary Kit for LPG, DGP or ISO pumps 6040.2302 6040.2301
Viper Capillary Kit for HPG pumps 6040.2309 6040.2308
Viper Capillary Kit for Biocompatible RS systems 6841.2301
MS Connection Kit for systems without UV detector 6720.0355 6720.0370
MS Connection Kit for systems with UV detector 6720.0365 6720.0375
MS Connection Kit for Vanquish systems with and without UV detector 6720.0405
Viper Solution Kit – Online SPE 6040.2802 6040.2801  
Viper Solution Kit – Tandem Operation (for UltiMate 3000 HPLC systems) or Tandem LC or LCMS (for Vanquish Duo UHPLC systems) 6040.2804 6040.2803 6036.2020
Viper Solution Kit – Parallel LC (for UltiMate 3000 HPLC systems) or Dual LC (for Vanquish Duo UHPLC systems) 6040.2810 6040.2809 6036.2305
Viper Solution Kit – Application Switching 6040.2806 6040.2805
Viper Solution Kit – Method Scouting 6040.2808 6040.2807 6036.2807
Viper Solution Kit – Method Scouting, ID 100 µm, biocompatible 6042.2807
Viper Solution Kit – Method Scouting, ID 180 µm, biocompatible 6042.2808
Viper Solution Kit – Inverse Gradient 6040.2819 6040.2820 6036.2010
UltiMate 3000 RSLCnano system

Maximize performance and simplify setup of your nano LC instrument with our workflow kits. These kits contain nanoViper fittings preassembled on UHPLC connection tubing and columns to enable easy, tool-free instrument setup and configuration. The fingertight fittings ensure virtually zero-dead-volume connections and provide UHPLC compatibility up to 1000 bar (14,500 psi).

Description Part number
Direct Injection nano LC kit 6720.0300
Direct Injection capillary LC kit 6720.0305
Preconcentration nano LC kit 6720.0310
Preconcentration capillary LC kit 6720.0315
Preconcentration micro LC kit 6720.0320
2D salt plugs kit 6720.0325
Tandem nano LC kit 6720.0335
MS Connection kit 6720.0345
OQ/PQ kit, NCS, NCp 6720.0360
EASY-Spray connection kit 6720.0395
Upgrade Kit ProFlow Flowmeter 6041.3003

Connectors, plugs and filters based on the Viper Fingertight fitting technology are available.

Description Part number
Blind Plug, biocompatible (titanium) 6040.2303
Union, SST 6040.2304
Calibration Tool 6040.2312
Inline Filter, titanium, av. frit pore size 0.5 µm 6036.1045

Select the passive pre-heater, column cooler, and tubing you need for your UHPLC or HPLC application.

Description Part number
Active Pre-heater, MP35N, ID × L, 100 µm × 380 mm (for Vanquish Column Compartment H) 6732.0110
Passive Pre-heater, MP35N, ID × L, 100 µm × 530 mm (for Vanquish Column Compartment H) 6732.0174
Post Column Cooler, MP35N, ID × L, 100 µm × 240 mm (for Vanquish Column Compartment H) 6732.0510
Passive Pre-heater, SST, ID, 100 µm (for TCC-3000) 6722.0510
Passive Pre-heater, SST, ID, 130 µm (for TCC-3000) 6722.0530
Passive Pre-heater, SST, ID, 180 µm (for TCC-3000) 6722.0540
Passive Pre-heater, SST, ID, 250 µm (for TCC-3000) 6722.0550
Passive Pre-heater, coated steel, biocompatible, ID, 130 µm (for TCC-3000) 6723.0232
Passive Pre-heater, coated steel, biocompatible, ID, 250 µm 7 µL (for TCC-3000) 6723.0242
Passive Pre-heater, coated steel, biocompatible, ID, 250 µm 11 µL (for TCC-3000) 6723.0252
Passive Pre-heater, MP35N, ID, 100 µm (for TCC-3000) 6723.0235
Passive Pre-heater, MP35N, ID, 180 µm (for TCC-3000) 6723.0245
Post Column Cooler, SST, ID, 130 µm (for TCC-3000) 6730.0008
MP35N thermally insulated, ID × L, 100 µm × 300 mm 6083.2405
MP35N thermally insulated, ID × L, 130 µm × 500 mm 6083.2411

Special nano LC capillaries are available to adjust setup for special application requirements.

Description Part number
nanoViper, ID × L, 20 µm × custom length 6041.5299
nanoViper, ID × L, 50 µm × custom length 6041.5599A
nanoViper, ID × L, 75 µm × custom length 6041.5799
nanoViper, thermally insulated, ID × L, 75 µm × 300 mm 6083.2415
UHPLC waste, ID × OD × L, 75 µm × 360 µm × 550 mm 6041.5289
Liquid Junction column out, ID × OD × L, 20 µm × 360 µm × 550 mm 6041.5290
Nano LC column to MS tubing, ID × OD × L, 20 µm × 280 µm × 1 m 6041.5292
Nano LC column to MS tubing, ID × OD × L, 20 µm × 360 µm × 1 m 6041.5293
Nano LC column to MS tubing, ID × OD × L, 50 µm × 280 µm × 1 m 6041.5294
Nano LC column to MS tubing, ID × OD × L, 50 µm × 360 µm × 1 m 6041.5295

This comprehensive guide includes flow schematics and all necessary part description for setting up easily your low-flow LC workflow.


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