Ideal for low-flow LC-MS workflows
Do you have applications requiring high sensitivity such as proteomics, metabolomics, and intact protein analysis? The Thermo Scientific EASY-nLC 1200 system provides effortless nanoflow UHPLC, and the Thermo Scientific UltiMate 3000 RSLCnano system provides fully integrated and versatile nano-, capillary-, and micro-flow solutions.
Browse our complete portfolio below, or let one of our experts help you tailor a selection specific to your application and workflow needs.
The right solution for every low-flow application
Experience outstanding performance and workflow versatility with the Thermo Scientific UltiMate 3000 RSLCnano and Thermo Scientific EASY-nLC 1200 systems. These low-flow UHPLC systems feature a wide flow-pressure footprint and are ready for nanoLC-MS with long columns, enabling deep dive proteomics experiments as well as high-throughput LC-MS analysis of large sample cohorts. The UltiMate 3000 RSLCnano and EASY-nLC 1200 systems offer excellent retention time precision, improved flow rate control, and intelligent operation so that you achieve greater confidence in your results.
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Choose from two low-flow UHPLC systems
A compact, integrated, and intuitive nanoLC system that offers effortless ultra-high performance for everyone. The EASY-nLC 1200 can handle ultra-long columns due to its high-pressure capability for maximum resolution of complex mixtures.
This versatile and customizable low-flow UHPLC platform offers flexibility for routine and advanced LCMS workflows. The UltiMate 3000 RSLCnano system offers excellent retention time precision and gradient accuracy for nano-, capillary, and micro-flow rates for more confidence in your LCMS results.
Low-flow UHPLC systems comparison
 EASY-nLC 1200 Operational simplicity and excellent performance |
 UltiMate 3000 RSLCnano Versatility and uncompromising precision |
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Performance |
System pressure |  |
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| Retention time precision | |
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| Splitless nano-flow | |||
| Nano-flow range | Recommended range: 100 nL/min–1.0 μL/min |
Recommended range: 50 nL/min–1.5 μL/min |
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| Capillary-flow range | – | Recommended range: 0.5–10 μL/min |
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| Micro-flow range | – | Recommended range: 5–50 μL/min |
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System Features |
Design | Integrated | Modular |
| Column compartment | n.a.* *Built-in heating with EASY-Spray columns |
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| Sample capacity |  |
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| Sample cooling | |||
| nanoViper fittings | |||
| Zero-loss sample pick-up | |||
| Nano-flow pump | High-pressure mixing binary syringe pump | High-pressure mixing binary continuous flow pump | |
| Analytical flow and loading pump | – | Low-pressure mixing ternary gradient pump | |
Application Setups |
Direct injection | ||
| Preconcentration | Vented column setup | Continuous direct flow by integrated loading pump | |
| 2D salt setups | – | ||
| Automated online 2D LC* | – | ||
| Offline fractionation* | – | ||
| Tandem LC* | – | ||
| *requires additional equipment | |||
Software |
Software | Xcalibur, LC Devices, & Tracefinder |
SII with Xcalibur & Tracefinder or Chromeleon Chromatography Data System |
| Integrated PC | – | ||
| Audit trail | – | ||
Accessories |
MS sources for MS integration | EASY-Spray Source Nanospray Flex Ion Source |
EASY-Spray Source Nanospray Flex Ion Source Ion Max Ion Source |
| Columns | EASY-Spray C18 Columns Integrated column/emitter design minimizing dead volume. Acclaim PepMap 100 C18 Columns Acclaim PepMap 300 C18 Columns Columns ideal for peptide and protein separations with inner diameters between 50 μm and 1 mm Any of our HPLC and UHPLC column |
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Connect with Thermo Scientific mass spectrometers for outstanding specificity and sensitivity.
- Orbitrap LC-MS
- Triple quadrupole LC-MS
- Ion Trap LC-MS
- Single quadrupole LC-MS
Thermo Scientific Viper and nanoViper HPLC fittings, connectors, tubing, and kits allow you to quickly connect HPLC modules, valves, and columns for leak-free connections that improve chromatographic results by minimizing dead volume. Viper and nanoViper fittings systems and kits require no tools for installation, fit narrow connections (e.g., 10-port valves), and combine simplicity with high performance.
- Connect your lab to boost productivity with secure remote access to your data and instruments
- Monitor your instruments in real time with automatic email notifications
- Store data in secure accounts and share with colleagues and collaborators
The AppsLab Library of Analytical Applications is a fully searchable online, analytical method repository where you can find applications with detailed method information, chromatograms and related compound information.
Optimize nanoflow LC/MS for proteomics applications with Thermo Scientific EASY-Spray C18 LC Columns.
In this webinar, scientists discuss the latest innovations in nano LC-MS technology with overviews of the EASY-nLC 1200 and versatile UltiMate 3000 RSLCnano systems.
