Do you use applications requiring high sensitivity such as proteomics, metabolomics, and intact protein analysis? The Thermo Scientific EASY-nLC 1200 system provides effortless nanoflow UHPLC, and the Thermo Scientific UltiMate 3000 RSLCnano system provides fully integrated nano-, capillary-, and micro-flow solutions.
For scientists who have restricted sample volumes and require high sensitivity and unsurpassed retention time precision, these low flow liquid chromatography (LC) technologies are the best solution. They use the latest technologies to provide you reliable nano-, capillary-, or micro-flow LC.
Our systems are equipped with easy-to-use Thermo Scientific nanoViper Fingertight Fittings, which allow you to quickly connect LC modules, valves, and columns without additional tools, for leak-free connections that can improve chromatographic results by minimizing dead volume.
Both the EASY-nLC 1200 system and the UltiMate 3000 RSLCnano system integrate seamlessly with Thermo Scientific mass spectrometers and EASY-Spray products for plug-and-spray simplicity.
A flexible, customizable system that meets all low flow applications spanning nano-, capillary-, and micro-flow needs. The UltiMate 3000 RSLCnano system provides excellent retention time precision for more confidence in your results.
A compact, integrated solution for nano LC-MS analyses, the EASY-nLC 1200 offers effortless nano-flow separations and is designed for intuitive operation with excellent performance. This system can handle ultra-long columns with its high pressure capability.
 EASY-nLC 1200 Operational simplicity and excellent performance |
 UltiMate 3000 RSLCnano Versatility and uncompromising precision |
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Performance |
System pressure |  |
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| Retention time precision | |
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| Splitless nano flow | |||
| Nano flow range | Recommended range: 100nL/min–1.5μL/min |
Recommended range: 50nL/min–1.5μL/min |
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| Capillary flow range | – | Recommended range: 0.5–10μL/min |
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| Micro flow range | – | Recommended range: 5–50μL/min |
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System Features |
Design | Integrated | Modular |
| Column compartment | n.a.* *Built-in heating with EASY-Spray columns |
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| Sample capacity |  |
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| Sample cooling | |||
| nanoViper fittings | |||
| Zero-loss sample pick-up | |||
| Nano flow pump | High pressure mixing binary syringe pump | High pressure mixing binary continuous flow pump | |
| Analytical flow and loading pump | – | Low pressure mixing ternary gradient pump | |
Application Setups |
Direct injection | ||
| Preconcentration | Vented column setup | Continuous direct flow by integrated loading pump | |
| 2D salt setups | – | ||
| Automated offline 2D LC* | – | ||
| Offline fractionation* | – | ||
| Tandem LC* | – | ||
| *requires additional equipment | |||
Software |
Software | Xcalibur, LC Devices, & Tracefinder |
SII with Xcalibur & Tracefinder or Chromeleon Chromatography Data System |
| Integrated PC | – | ||
| Audit trail | – | ||
Accessories |
MS sources for MS integration | EASY-Spray Source Nanospray Flex Ion Source |
EASY-Spray Source Nanospray Flex Ion Source Ion Max Ion Source |
| Columns | EASY-Spray C18 Columns Integrated column/emitter design minimizing dead volume. Acclaim PepMap 100 C18 Columns Acclaim PepMap 300 C18 Columns Columns ideal for peptide and protein separations with inner diameters between 50μm to 1mm Any of our HPLC and UHPLC column |
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Connect with Thermo Scientific mass spectrometers for outstanding specificity and sensitivity.
The AppsLab Library of Analytical Applications is a fully searchable online, analytical method repository where you can find applications with detailed method information, chromatograms and related compound information.
Optimize nanoflow LC/MS for proteomics applications with Thermo Scientific EASY-Spray C18 LC Columns.
In this webinar, scientists discuss the latest innovations in nano LC-MS technology with overviews of the EASY-nLC 1200 and versatile UltiMate 3000 RSLCnano systems.
