Ideal for low-flow LC-MS workflows
Do you have applications requiring high sensitivity such as proteomics, metabolomics, and intact protein analysis? The Thermo Scientific EASY-nLC 1200 system provides effortless nanoflow UHPLC, and the Thermo Scientific UltiMate 3000 RSLCnano system provides fully integrated and versatile nano-, capillary-, and micro-flow solutions.
Browse our complete portfolio below, or let one of our experts help you tailor a selection specific to your application and workflow needs.
The right solution for every low-flow application
Experience outstanding performance and workflow versatility with the Thermo Scientific UltiMate 3000 RSLCnano and Thermo Scientific EASY-nLC 1200 systems. These low-flow UHPLC systems feature a wide flow-pressure footprint and are ready for nanoLC-MS with long columns, enabling deep dive proteomics experiments as well as high-throughput LC-MS analysis of large sample cohorts. The UltiMate 3000 RSLCnano and EASY-nLC 1200 systems offer excellent retention time precision, improved flow rate control, and intelligent operation so that you achieve greater confidence in your results.
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Choose from two low-flow UHPLC systems
This versatile and customizable low-flow UHPLC platform offers flexibility for routine and advanced LCMS workflows. The UltiMate 3000 RSLCnano system offers excellent retention time precision and gradient accuracy for nano-, capillary, and micro-flow rates for more confidence in your LCMS results.
Low-flow UHPLC systems comparison
Operational simplicity and excellent performance
UltiMate 3000 RSLCnano
Versatility and uncompromising precision
|Retention time precision|
|Nano-flow range||Recommended range:
100 nL/min–1.0 μL/min
50 nL/min–1.5 μL/min
|Capillary-flow range||–||Recommended range:
|Micro-flow range||–||Recommended range:
*Built-in heating with
|Zero-loss sample pick-up|
|Nano-flow pump||High-pressure mixing binary syringe pump||High-pressure mixing binary continuous flow pump|
|Analytical flow and loading pump||–||Low-pressure mixing ternary gradient pump|
|Preconcentration||Vented column setup||Continuous direct flow by integrated loading pump|
|2D salt setups||–|
|Automated online 2D LC*||–|
|*requires additional equipment|
|Software||Xcalibur, LC Devices,
|SII with Xcalibur & Tracefinder
|MS sources for MS integration||EASY-Spray Source
Nanospray Flex Ion Source
Nanospray Flex Ion Source
Ion Max Ion Source
|Columns||EASY-Spray C18 Columns
Integrated column/emitter design minimizing dead volume.
Acclaim PepMap 100 C18 Columns
Acclaim PepMap 300 C18 Columns
Columns ideal for peptide and protein separations with inner
diameters between 50 μm and 1 mm
Any of our HPLC and UHPLC column
|= Not applicable||= Poor||= Good||= Better||= Best|
Thermo Scientific Viper and nanoViper HPLC fittings, connectors, tubing, and kits allow you to quickly connect HPLC modules, valves, and columns for leak-free connections that improve chromatographic results by minimizing dead volume. Viper and nanoViper fittings systems and kits require no tools for installation, fit narrow connections (e.g., 10-port valves), and combine simplicity with high performance.
- Connect your lab to boost productivity with secure remote access to your data and instruments
- Monitor your instruments in real time with automatic email notifications
- Store data in secure accounts and share with colleagues and collaborators
In this webinar, scientists discuss the latest innovations in nano LC-MS technology with overviews of the EASY-nLC 1200 and versatile UltiMate 3000 RSLCnano systems.