Nano, Capillary, and Microflow UHPLC Systems

Ideal for low-flow LC-MS workflows

Do you use applications requiring high sensitivity such as proteomics, metabolomics, and intact protein analysis? The Thermo Scientific EASY-nLC 1200 system provides effortless nanoflow UHPLC, and the Thermo Scientific UltiMate 3000 RSLCnano system provides fully integrated nano-, capillary-, and micro-flow solutions.

Browse our complete portfolio below, or let one of our experts help you tailor a selection specific to your application and workflow needs.

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The right solution for every low-flow application

For scientists who have restricted sample volumes and require high sensitivity and unsurpassed retention time precision, these low flow liquid chromatography (LC) technologies are the best solution. They use the latest technologies to provide you reliable nano-, capillary-, or micro-flow LC.

Our systems are equipped with easy-to-use Thermo Scientific nanoViper Fingertight Fittings, which allow you to quickly connect LC modules, valves, and columns without additional tools, for leak-free connections that can improve chromatographic results by minimizing dead volume.

Both the EASY-nLC 1200 system and the UltiMate 3000 RSLCnano system integrate seamlessly with Thermo Scientific mass spectrometers and EASY-Spray products for plug-and-spray simplicity.

Choose from two UHPLC systems

Systems comparison

    EASY-nLC 1200

EASY-nLC 1200

Operational simplicity and excellent performance
UltiMate 3000 RSLCnano

UltiMate 3000 RSLCnano

Versatility and uncompromising precision


System pressure
Retention time precision
Splitless nano flow    
Nano flow range Recommended range:
Recommended range:
Capillary flow range Recommended range:
Micro flow range Recommended range:

System Features

Design Integrated Modular
Column compartment n.a.*
*Built-in heating with
EASY-Spray columns
Sample capacity
Sample cooling    
nanoViper fittings    
Zero-loss sample pick-up    
Nano flow pump High pressure mixing binary syringe pump High pressure mixing binary continuous flow pump
Analytical flow and loading pump Low pressure mixing ternary gradient pump

Application Setups

Direct injection    
Preconcentration Vented column setup Continuous direct flow by integrated loading pump
2D salt setups  
Automated offline 2D LC*  
Offline fractionation*  
Tandem LC*  
*requires additional equipment


Software Xcalibur, LC Devices,
& Tracefinder
SII with Xcalibur & Tracefinder
Chromeleon Chromatography
Data System
Integrated PC  
Audit trail  


MS sources for MS integration EASY-Spray Source
Nanospray Flex Ion Source
EASY-Spray Source
Nanospray Flex Ion Source
Ion Max Ion Source
Columns EASY-Spray C18 Columns
Integrated column/emitter design minimizing dead volume.

Acclaim PepMap 100 C18 Columns
Acclaim PepMap 300 C18 Columns
Columns ideal for peptide and protein separations with inner
diameters between 50μm to 1mm

Any of our HPLC and UHPLC column
= Not applicable = Poor = Good = Better = Best

Discover the best HPLC systems for your applications and budget

Discover the best HPLC system for your applications and budget

Use these tools to help you decide

HPLC Systems Selection Guide
Use this tool to rank the top HPLC instruments for your applications based on your responses to simple pull-down menus.

HPLC System Total Cost of Ownership Calculator
Use this tool to estimate your cost for purchasing and running Thermo Scientific HPLC and UHPLC systems. You can compare costs between instruments.

LC Method Transfer Calculator
Use this online calculator to transfer methods from HPLC to UHPLC conditions. Calculate the new gradient table, adjust sample volumes and run times if applicable.

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