Vanquish UHPLC Systems

Separate your science from the status quo

Thermo Scientific Vanquish HPLC and UHPLC systems are the most advanced liquid chromatography instruments available. The Vanquish LC platform improves performance, repeatability, and dependability with no trade-offs in quality or ease-of-use. Vanquish LC platform users have all they need to solve their toughest analytical challenges with confidence.

Advanced LC systems for solving tough analytical challenges

For any application—whether HPLC or UHPLC, small or large molecules, simple or complex mixtures—the Vanquish LC platform was designed to handle it all. This innovative, simple-to-operate, and easy-to-maintain platform delivers confident separations that push chromatographic boundaries and continually deliver robust results. Regardless of the configuration you choose, you will get a highly integrated solution with optimal fluidic connections, providing unsurpassed retention time and peak area precision.

You will also benefit from the industry-leading, easy-to-use, and compliant Thermo Scientific Chromeleon Chromatography Data System (CDS) with eWorkflow method setup, dedicated ePanels, Open Access, and smart tools. Chromeleon CDS delivers advanced system communication with single-point intelligent control and functionality. It also provides direct plugins for the Vanquish system to other manufacturers' chromatography data systems.

Choose your Vanquish UHPLC systems

For your most complex and difficult samples, or for applications where you are aiming for the shortest cycle times and highest throughput. Vanquish Horizon UHPLC system allows for smaller particles and column dimensions to be run with greater confidence for the most demanding LC and LC-MS workflows.

For assays that require more flexibility in solvents to accommodate all applications. Vanquish Flex UHPLC systems excel from method development to routine analyses. With binary and quaternary pump options, the Vanquish Flex UHPLC systems offer outstanding performance for targeted LC and LC-MS workflows.

Increase productivity, up-time, and sample knowledge for a faster return on investment. Vanquish Duo UHPLC systems expands the benefits of the Vanquish platform through two flow paths to support three workflows (dual, tandem and inverse gradient) within one integrated UHPLC system. With the addition of the second flow path, improve your productivity by saving time, cost per sample without added bench space, and without compromising performance, robustness or ease-of-use.

Compare Vanquish UHPLC systems
    Vanquish Horizon
UHPLC System
Vanquish Flex
UHPLC Systems
Vanquish Core
HPLC Systems
    Unrivaled performance and throughput for applications requiring high-end UHPLC Binary or quaternary solvent blending for fast and reliable UHPLC or flexible method development Remove any disruptions from your routine HPLC analyses


System pressure
Up to 1500 bar

Up to 1000 bar

Up to 700 bar
Retention time precision
Flow range
5 mL/min

8 mL/min

10 mL/min
Biocompatibility     N/A
Design Modular

System components

Pump Vanquish Binary Pump H
The pump with the highest backpressure at 1500 bar, and ideal for any gradient performance from shallow to ballistic
Vanquish Binary Pump F
UHPLC performance for demanding routine applications up to 1000 bar

Vanquish Quaternary
Pump F

UHPLC performance with more solvent flexibility up to 1000 bar
Vanquish Binary
Pump C

Simplicity and throughput for routine HPLC applications up to 700 bar

Vanquish Quaternary
Pump C

Flexibility and dependability for routine HPLC applications up to 700 bar

Vanquish Isocratic
Pump C

Simplicity for basic HPLC applications up to 700 bar
Autosampler Vanquish Split
Sampler HT

Excellent precision, accuracy, and short cycle times with innovative air-stream cooling and barcode reading for inventory management for all workflows up to 1500 bar
Vanquish Split
Sampler FT

Enjoy the same benefits as the Vanquish Split Sampler HT for applications up to 1000 bar
Vanquish Dual Split Sampler C/CT
Delivering extreme dependability and reliability for routine HPLC applications up to 700 bar. Optional thermostatting for samples.
Extended sample capacity Vanquish Charger
An intelligent, robotic rack and plate feeder that automatically loads samples in the autosampler and extends the sample capacity of all Vanquish samplers to up to 8832 samples (23 wellplates or 12 vial racks) with innovative air-stream cooling and barcode reading for inventory management
Column compartment Vanquish Column Compartment H
Provides two column thermostatting modes (still and forced air), active and passive solvent pre-heating, and post-column cooling for optimized control of your separations and advanced method transfer. Temperature range: 5–120 °C
Vanquish Column Compartment C
High performance and flexibility in thermostatting mode to simplify method transfer from any HPLC system. Temperature range: 5–85 °C
Virtually zero dead volume system connections Viper UHPLC Fittings Kits
Order all the UHPLC fittings and capillaries you need for your system or application in a single kit
= Not applicable = Poor = Good = Better = Best

* Different combinations of Vanquish pumps or autosamplers can be used for desired configuration

Compatible components for all Vanquish UHPLC systems

Detector options Vanquish columns Chromeleon CDS
Extend pressure capabilities and robustness by using columns that were developed for Vanquish systems, allowing you to separate your science better, faster, and easier than ever before.
All Vanquish systems can be used with any of our HPLC and UHPLC columns
  • Streamlines your data processing
  • Industry-leading ease of use and intelligent functionalities
  • Outstanding data integrity
  • Unique user guidance and hardware automation features designed for Vanquish systems

Discover the best HPLC systems for your applications and budget

Discover the best HPLC system for your applications and budget

Use these tools to help you decide

HPLC Systems Selection Guide
Use this tool to rank the top HPLC instruments for your applications based on your responses to simple pull-down menus.

HPLC System Total Cost of Ownership Calculator
Use this tool to estimate your cost for purchasing and running Thermo Scientific HPLC and UHPLC systems. You can compare costs between instruments.

LC Method Transfer Calculator
Use this online calculator to transfer methods from HPLC to UHPLC conditions. Calculate the new gradient table, adjust sample volumes and run times if applicable.

Featured Vanquish UHPLC system videos
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