Simultaneous Quantitation and Discovery (SQUAD)

Gain deeper understanding of biological systems through a single experiment. Measure hypothesis-driven lists of targets while simultaneously collecting untargeted discovery data with depth, accuracy, and precision. You can then reanalyze or retro-mine data to unveil global metabolic changes that were not initially the main focus.

Simultaneous Quantitation and Discovery (SQUAD) analysis workflows leverage the advantages of Thermo Scientific Orbitrap mass spectrometers. The Thermo Scientific Orbitrap Exploris series offers fast polarity switching, which yields a broad coverage of metabolites. The Thermo Scientific Orbitrap Tribrid instruments contain two detectors used in parallel for efficient merging of both targeted and untargeted studies. For high-throughput and maximum annotations, the novel Thermo Scientific Orbitrap Astral Mass Spectrometer unlocks additional insights into biology and disease mechanisms.