Our LanthaScreen® kinase activity assay toolbox of "mix and match" assay reagents is designed to enable rapid assay configuration. Our portfolio of phospho-specific antibodies, matched fluorescent peptide substrates and in-house produced kinases have been tested in >200 different kinase assays to date.

Phospho-specific Antibodies with Peptide Substrates

To facilitate in-house kinase assay configuration and screening, we have developed a selection of Tb-labeled phospho-specific antibodies, which may be accessed in small scale and screening quantities.  Matched and validated substrates are available for kinase assay configuration.

These antibodies may also be available with Europium labels, contact us at discoverysciences@invitrogen.com, for more information.

Tyrosine Kinase Assays

SKU Product Catalog Size
PV5692 LanthaScreen® Eu-PY20 Antibody Kit 25μg
PV5696 LanthaScreen® Eu-PY20 Antibody Kit 1mg
PV5693 Alexa Fluor® 647-Poly GT
1 nmol
PV5836 Alexa Fluor® 647-Poly GT 10 nmol
PV3552 LanthaScreen® Tb-PY20 Antibody Kit 25μg
PV3553 LanthaScreen® Tb-PY20 Antibody Kit 1mg
PV3610 Fluorescein-Poly GT, 30 μM 1mL
PV3611 Fluorescein-Poly GAT, 30 μM 1mL

Serine/Threonine Kinase Assays

SKU Product Catalog Size
PV3560 LanthaScreen® Tb-pSer (PKC) Antibody Kit 25μg
PV3561 LanthaScreen® Tb-pSer (PKC) Antibody Kit 1mg
PV3506 Fluorescein-PKC Substrate 1mg
PV3562 LanthaScreen® Tb-IKBa [pSer32] Antibody Kit 25μg
PV3563 LanthaScreen® Tb-IKBa [pSer32] Antibody Kit 1mg
PV3507 Fluorscein-IKK substrate 1mg
PV3564 LanthaScreen® Tb-pCrosstide Antibody Kit 25μg
PV3565 LanthaScreen® Tb-pCrosstide Antibody Kit 1mg
PV3509 Fluorescein-Crosstide Substrate 1mg
PV3542 LanthaScreen® Tb-CREB pSer133 Antibody
25μg
PV3508 Fluorescein-CREBtide Substrate, 1 mg/mL 1mg
PV4899 LanthaScreen® Tb-pERM (pLRRKtide) Antibody Kit 25μg
PV4900 LanthaScreen® Tb-pERM (pLRRKtide) Antibody Kit 1mg
PV4901 Fluorescein-ERM (LRRKtide) Substrate 1mg
PV5130 LanthaScreen® Tb-anti-phospho-p53 [pSer15] Antibody Kit 25μg
PV5131 LanthaScreen® Tb-anti-phospho-p53 [pSer15] Antibody Kit 1mg
PV5132 Fluorescein-p53 [Ser15] Substrate 1mg

Phospho-specific Antibodies with Physiological Protein Substrates

As an extension to our peptide-based substrates for LanthaScreen® kinase activity assays, we now offer physiological protein substrates that are directly labeled with a green fluorescent protein (GFP) acceptor fluorophore. Pairing the GFP-labeled substrate with a corresponding terbium (Tb)-labeled phosphospecific antibody allows detection of the phosphorylated product.

Ordering Information

SKU Product Catalog Size
PV4451 LanthaScreen® Tb-ATF2 [pSer71] Antibody Kit 25μg
PV4452 LanthaScreen® Tb-ATF2 [pSer71] Antibody Kit 1mg
PV4445 GFP-ATF2 (19-96) 20 nmol
PV4453 LanthaScreen® Tb-c-Jun [pSer73] Antibody Kit 25μg
PV4454 LanthaScreen® Tb-c-Jun [pSer73] Antibody Kit 1mg
PV4446 GFP-c-jun (1-179) 20 nmol
PV4757 LanthaScreen® Tb-4E-BP1 [pThr46] Antibody Kit 25μg
PV4758 LanthaScreen® Tb-4E-BP1 (pThr46) Antibody Kit 1mg
PV4759 GFP-4E-BP1 20 nmol
PV4810 LanthaScreen® Tb-eIF2a [pSer52] Antibody Kit 25μg
PV4811 LanthaScreen® Tb-eIF2a [pSer52] Antibody Kit 1mg
PV4809 GFP-eIF2a 20 nmol
PV4813 LanthaScreen® Tb-MAP2K1 [pSer217/221] Antibody Kit 25μg
PV4814 LanthaScreen® Tb-MAP2K1 [pSer217/221] Antibody Kit 1mg
PV4812 Fluorescein-MEK1 20 nmol
PV4844 LanthScreen® Tb-anti-STAT1 [pTyr701] Antibody Kit 25μg
PV4845 LanthScreen® Tb-anti-STAT1 [pTyr701] Antibody Kit 1mg
PV5211 GFP-STAT1 20 nmol
PV4846 LanthaScreen® Tb-STAT3 [pTyr705] Antibody Kit 25μg
PV4847 LanthaScreen® Tb-STAT3 [pTyr705] Antibody Kit 1mg
PV5212 GFP-STAT3 20 nmol

Buffers and Other Supplemental Assay Reagents

To complete our offering of antibodies and substrates for the development of TR-FRET kinase assays, we offer a selection of validated kinase buffers and other assay reagents.

Ordering Information

SKU Product Catalog Size
PV3574 TR-FRET Dilution Buffer 100mL
PV3189 5X Kinase Buffer A 4mL
PV5125 5X Kinase Buffer Q 4mL
PV5101 5X Kinase Buffer R 4mL
PV5213 5X Kinase Buffer S 4mL
PV4794 1X mTOR Assay Buffer 20mL
P2825 Kinase Quench Buffer 1mL
PV3227 10 mM ATP 500μL
P2325 1M DTT 1mL