Tools that help analyze compound efficacy in a pathway-specific physiological context are essential for drug discovery research. To address this need, Life Technologies offers three technology platforms for endpoint or proximity-oriented pathway analysis for use in high-throughput screening and profiling.

To compile the components needed to make the right cellular assay for your research, use our new Drug Discovery Assay Maker web tool.

Request Assay Information        Use Assay Maker

Choose the right cellular pathway analysis assay for your research

  Analysis of an entire
signal transduction
pathway with a single
end-point readout
  Detection of post-translational modifications of specific protein substrates within a native cellular environment   Detection of post-translational modifications of specific protein substrates in the cell background of your choice
 
 
 
 
 
 
Stable vs. Transient Stable   Stable   Transient
Cellular engineering Pathway-specific response element upstream of BLA reporter   GFP-substrate fusion, readout of endogenous kinases   GFP-substrate fusion, readout of endogenous kinases
Assay optimization required? No, already optimized   No, already optimized   Yes, if using cell backgrounds other than recommended
Detection technology FRET (coumarin to fluorescein)   TR-FRET (terbium-labeled antibody to GFP-substrate fusion)   TR-FRET (terbium-labeled antibody to GFP-substrate fusion)
Antibody-based assay?     yes   yes
Total assay volume (384-well) 40 µL   40 µL   40 µL
Overcoming interference Background suppression dye in the substrate mixture   Time-resolved readout allows read after interfering signals have decayed   Time-resolved readout allows read after interfering signals have decayed
Plate reader method Bottom-read   Top-read   Top-read
Cost per well $   $$–$$$   $$–$$$
Use in SelectScreen® Cellular Profiling Services yes   yes   yes
  Request Info   Request Info   Request Info

Elucidate your target pathways with multiple assay formats

Life Technologies offers three assay technologies for your cellular pathway analysis research.