Tools that help analyze compound efficacy in a pathway-specific physiological context are essential for drug discovery research. To address this need, Life Technologies offers three technology platforms for endpoint or proximity-oriented pathway analysis for use in high-throughput screening and profiling.
To compile the components needed to make the right cellular assay for your research, use our new Drug Discovery Assay Maker web tool.
|Analysis of an entire|
pathway with a single
|Detection of post-translational modifications of specific protein substrates within a native cellular environment||Detection of post-translational modifications of specific protein substrates in the cell background of your choice|
|Stable vs. Transient||Stable||Stable||Transient|
|Cellular engineering||Pathway-specific response element upstream of BLA reporter||GFP-substrate fusion, readout of endogenous kinases||GFP-substrate fusion, readout of endogenous kinases|
|Assay optimization required?||No, already optimized||No, already optimized||Yes, if using cell backgrounds other than recommended|
|Detection technology||FRET (coumarin to fluorescein)||TR-FRET (terbium-labeled antibody to GFP-substrate fusion)||TR-FRET (terbium-labeled antibody to GFP-substrate fusion)|
|Total assay volume (384-well)||40 µL||40 µL||40 µL|
|Overcoming interference||Background suppression dye in the substrate mixture||Time-resolved readout allows read after interfering signals have decayed||Time-resolved readout allows read after interfering signals have decayed|
|Plate reader method||Bottom-read||Top-read||Top-read|
|Cost per well||$||$$–$$$||$$–$$$|
|Use in SelectScreen® Cellular Profiling Services|
|Request Info||Request Info||Request Info|
Life Technologies offers three assay technologies for your cellular pathway analysis research.
For Research Use Only. Not for use in diagnostic procedures.