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Tools that help analyze compound efficacy in a pathway-specific physiological context are essential for drug discovery research. To address this need, Life Technologies offers three technology platforms for endpoint or proximity-oriented pathway analysis for use in high-throughput screening and profiling.

To compile the components needed to make the right cellular assay for your research, use our new Drug Discovery Assay Maker web tool.

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Choose the right cellular pathway analysis assay for your research

 Analysis of an entire
signal transduction
pathway with a single
end-point readout
 Detection of post-translational modifications of specific protein substrates within a native cellular environment Detection of post-translational modifications of specific protein substrates in the cell background of your choice
 
 
 
 
 
 
Stable vs. TransientStable  Stable  Transient
Cellular engineeringPathway-specific response element upstream of BLA reporter GFP-substrate fusion, readout of endogenous kinases GFP-substrate fusion, readout of endogenous kinases
Assay optimization required?No, already optimized No, already optimized Yes, if using cell backgrounds other than recommended
Detection technologyFRET (coumarin to fluorescein) TR-FRET (terbium-labeled antibody to GFP-substrate fusion) TR-FRET (terbium-labeled antibody to GFP-substrate fusion)
Antibody-based assay?  yes yes
Total assay volume (384-well)40 µL 40 µL 40 µL
Overcoming interferenceBackground suppression dye in the substrate mixture Time-resolved readout allows read after interfering signals have decayed Time-resolved readout allows read after interfering signals have decayed
Plate reader methodBottom-read Top-read Top-read
Cost per well$ $$–$$$ $$–$$$
Use in SelectScreen® Cellular Profiling Servicesyes yes yes
 Request Info Request Info Request Info

Elucidate your target pathways with multiple assay formats

Life Technologies offers three assay technologies for your cellular pathway analysis research.

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