showcase.par.65443.image.0.0.1

Tools that help analyze compound efficacy in a pathway-specific physiological context are essential for drug discovery research. To address this need, Life Technologies offers three technology platforms for endpoint or proximity-oriented pathway analysis for use in high-throughput screening and profiling.

To compile the components needed to make the right cellular assay for your research, use our new Drug Discovery Assay Maker™ web tool.

Request Assay Information        Use Assay Maker™

Choose the right cellular pathway analysis assay for your research

  Analysis of an entire
signal transduction
pathway with a single
end-point readout
Detection of post-translational modifications of specific protein substrates within a native cellular environment Detection of post-translational modifications of specific protein substrates in the cell background of your choice
Stable vs. Transient Stable Stable Transient
Cellular engineering Pathway-specific response element upstream of BLA reporter GFP-substrate fusion, readout of endogenous kinases GFP-substrate fusion, readout of endogenous kinases
Assay optimization required? No, already optimized No, already optimized Yes, if using cell backgrounds other than recommended
Detection technology FRET (coumarin to fluorescein)TR-FRET (terbium-labeled antibody to GFP-substrate fusion) TR-FRET (terbium-labeled antibody to GFP-substrate fusion)
Antibody-based assay? yes yes
Total assay volume (384-well) 40 µL 40 µL 40 µL
Overcoming interference Background suppression dye in the substrate mixture Time-resolved readout allows read after interfering signals have decayed Time-resolved readout allows read after interfering signals have decayed
Plate reader method Bottom-readTop-readTop-read
Cost per well $$$ - $$$$$ - $$$
Use in SelectScreen® Cellular Profiling Services yesyesyes
Request Info Request Info Request Info

Elucidate your target pathways with multiple assay formats

Life Technologies offers three assay technologies for your cellular pathway analysis research.