Immunization and egg-collection procedures for custom chicken polyclonal antibody production
Our comprehensive array of custom antibody services includes a customizable option for chicken immunization, egg-collection, and titer analysis for chicken antibody production, plus several extension possibilities.
Highlights
- Custom chicken antibodies—a reliable protocol for immunizing chickens, collecting eggs, testing bleeds, reporting titer results, and delivering eggs
- Full-service integration—combine this immunization service with our complete set of services for antigen preparation and antibody purification (see Custom Polyclonal Antibodies pages for details)
This chicken immunization schedule uses two White Leghorn chickens. Animals are housed individually in separate cages, injections are subcutaneous (SQ) as emulsions in Complete Freund’s Adjuvant (CFA) or Incomplete Freund’s Adjuvant (IFA); alternative adjuvants can be used upon request. Customizations to the protocol are available. For example, you can choose the number of chickens needed and you can adjust the length of your protocol beyond the 80-day immunization schedule to meet your research goals. Please contact us to discuss the options in detail. Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before day 0. Protocols days are approximate (± 2 days).
| Procedure | Protocol day | Description |
|---|---|---|
| Control serum collection | Day 0 | Pre-immune bleed (~2mL per chicken) |
| Primary injection | Day 1 | Immunize with 0.25 mg antigen in CFA, SQ 4 sites |
| 1st booster | Day 14 | Boost with 0.10 mg antigen in IFA, SQ 4 sites |
| Egg collection | Day 21 | Collect eggs for one week |
| 2nd booster | Day 42 | Boost with 0.10 mg antigen in IFA, SQ 4 sites |
| Egg collection | Day 48 | Collect eggs for one week |
| Serum collection | Day 56 | Test-bleed (~1-2 mL per chicken) |
| ELISA and shipping | Day 65 | ELISA titration (results available online); Verify disposition of chickens; decide to continue or terminate |
| Instruction due date | Day 80 | If no animal instructions are received, per diem charges will apply |
Deliverables (Day 65): 32 vials
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