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Immunization and egg-collection procedures for custom chicken polyclonal antibody production

Our comprehensive array of custom antibody services includes a customizable option for chicken immunization, egg-collection, and titer analysis for chicken antibody production, plus several extension possibilities.

Custom Antibody Request

Highlights

  • Custom chicken antibodies—a reliable protocol for immunizing chickens, collecting eggs, testing bleeds, reporting titer results, and delivering eggs
  • Full-service integration—combine this immunization service with our complete set of services for antigen preparation and antibody purification (see Custom Polyclonal Antibodies pages for details)
2-chicken 80-day chicken immunization protocol

This chicken immunization schedule uses two White Leghorn chickens. Animals are housed individually in separate cages, injections are subcutaneous (SQ) as emulsions in Complete Freund’s Adjuvant (CFA) or Incomplete Freund’s Adjuvant (IFA); alternative adjuvants can be used upon request. Customizations to the protocol are available. For example, you can choose the number of chickens needed and you can adjust the length of your protocol beyond the 80-day immunization schedule to meet your research goals. Please contact us to discuss the options in detail. Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before day 0. Protocols days are approximate (± 2 days).

ProcedureProtocol dayDescription
Control serum collectionDay 0Pre-immune bleed (~2mL per chicken)
Primary injectionDay 1Immunize with 0.25 mg antigen in CFA, SQ 4 sites
1st boosterDay 14Boost with 0.10 mg antigen in IFA, SQ 4 sites
Egg collectionDay 21Collect eggs for one week
2nd boosterDay 42Boost with 0.10 mg antigen in IFA, SQ 4 sites
Egg collectionDay 48Collect eggs for one week
Serum collectionDay 56Test-bleed (~1-2 mL per chicken)
ELISA and shippingDay 65ELISA titration (results available online);
Verify disposition of chickens; decide to continue or terminate
Instruction due dateDay 80If no animal instructions are received, per diem charges will apply

Deliverables (Day 65): 32 vials

  • 2 vials: 2 mL per vial pre-immune sera
  • 2 vials: 1-2 mL test bleed post-inject sera (Day 56)
  • 28 vials: post-inject eggs (2 week egg collection)