Immunization and serum-collection procedures for custom rat polyclonal antibody production
Our comprehensive array of Thermo Scientific Pierce custom antibody services includes a customizable option for rat immunization, serum-collection, and titer analysis for rat antibody production, plus extension possibilities.
- Custom rat antibodies—a reliable protocol for immunizing rats, collecting serum, testing bleeds, reporting titer results and delivering antisera
- Track and customize project—track and manage projects online with the OpenProject Tool: you decide which animals to continue or terminate
- Full service integration—combine this immunization service with our complete set of services for antigen preparation and antibody purification
This rat immunization schedule uses two Sprague Dawley rats for each MAP-peptide, conjugated-peptide antigen, or protein immunogen. Animals are housed individually in separate cages. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used upon request.
80-Day 2 Rat Immunization Protocol
Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Protocol days are approximate (± 2 days).
|Control serum collection||Day 0||Pre-immune bleed (~1 mL per rat)|
|Primary injection||Day 1||Immunize with 100 µg antigen in CFA, SQ 4 sites|
|1st booster||Day 21||Boost with 50 µg antigen in IFA, SQ 4 sites|
|2nd booster||Day 42||Boost with 50 µg antigen in IFA, SQ 4 sites|
|Test bleed||Day 50||Test bleed (~1 mL per rat)|
|ELISA and shipping||Day 60||ELISA titration of pre-immune and test bleed;
(results available online)
|3rd booster||Day 62||Boost with 50 µg antigen in IFA, SQ 4 sites|
|Decision||Day 64||Verify disposition of rats; decide to continue or terminate|
|Instruction due date||Day 80||Terminal bleed (exsanguination, yielding 2–4 mL per rat)
and termination of animals if instructions not received
Deliverables: 6 vials crude antibody sera
Tools and information
For Research Use Only. Not for use in diagnostic procedures.