Our comprehensive array of custom antibody services includes a customizable option for goat immunization, serum collection, and titer analysis for goat antibody production, plus several extension possibilities.
This goat immunization schedule uses two mixed-breed goats (usually Boer/Nubian or Alpine, unless specifically requested) for each immunogen. Injections are intradermal (ID) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used upon request. Customizations to the protocol are available. For example, you can choose the number of goats needed and you can adjust the length of your protocol beyond the 120-day immunization schedule to meet your research goals. Please contact us to discuss the options in detail. Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Protocol Days are approximate (± 2 days).
|Control serum collection||Day 0||Pre-immune bleed (~5 mL per goat)|
|Primary injection||Day 1||Immunize with 0.50 mg antigen in CFA, ID 20 sites|
|1st booster||Day 14||Boost with 0.50 mg antigen in IFA, ID 20 sites|
|2nd booster||Day 28||Boost with 0.50 mg antigen in IFA, ID 20 sites|
|Serum collection||Day 42||Test-bleed (~5 mL) per goat|
|3rd booster||Day 56||Boost with 0.50 mg antigen in IFA, ID 20 sites|
|Serum collection||Day 56||Test-bleed (~5 mL) per goat|
|4th booster||Day 70||Boost with 0.50 mg antigen in IFA, ID 20 sites|
|5th booster||Day 84||Boost with 0.50 mg antigen in IFA, ID 20 sites|
|Serum collection||Day 84||Test-bleed (~5 mL) per goat|
|6th booster||Day 98||Boost with 0.50 mg antigen in IFA, ID 20 sites|
|7th booster||Day 112||Boost with 0.50 mg antigen in IFA, ID 20 sites|
|Serum collection||Day 112||Test-bleed (~5mL) per goat|
|ELISA and shipping||Day 118||ELISA titration (results available online);|
Verify disposition of goats; decide to continue or terminate;
Plasmapheresis from one goat (~1,000 mL serum),
taken when requested by customer
|Instruction due date||Day 120||If no animal instructions are received, per diem charges will apply;|
Extension protocol is recommended with titer monitoring by ELISA
|Deliverables: 22 vials crude antibody sera
For Research Use Only. Not for use in diagnostic procedures.