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Immunization and serum-collection procedures for custom goat polyclonal antibody production

Our comprehensive array of custom antibody services includes a customizable option for goat immunization, serum collection, and titer analysis for goat antibody production, plus several extension possibilities.

Custom Antibody Request

Highlights

  • Custom goat antibodies—a reliable protocol for immunizing goats, collecting serum, testing bleeds, reporting titer results, and delivering plasma
  • Full-service integration—combine this immunization service with our complete set of services for antigen preparation and antibody purification
2-goat 120-day immunization protocol

This goat immunization schedule uses two mixed-breed goats (usually Boer/Nubian or Alpine, unless specifically requested) for each immunogen. Injections are intradermal (ID) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used upon request. Customizations to the protocol are available. For example, you can choose the number of goats needed and you can adjust the length of your protocol beyond the 120-day immunization schedule to meet your research goals. Please contact us to discuss the options in detail. Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Protocol Days are approximate (± 2 days).

ProcedureProtocol dayDescription
Control serum collectionDay 0Pre-immune bleed (~5 mL per goat)
Primary injectionDay 1Immunize with 0.50 mg antigen in CFA, ID 20 sites
1st boosterDay 14Boost with 0.50 mg antigen in IFA, ID 20 sites
2nd boosterDay 28Boost with 0.50 mg antigen in IFA, ID 20 sites
Serum collectionDay 42Test-bleed (~5 mL) per goat
3rd boosterDay 56Boost with 0.50 mg antigen in IFA, ID 20 sites
Serum collectionDay 56Test-bleed (~5 mL) per goat
4th boosterDay 70Boost with 0.50 mg antigen in IFA, ID 20 sites
5th boosterDay 84Boost with 0.50 mg antigen in IFA, ID 20 sites
Serum collectionDay 84Test-bleed (~5 mL) per goat
6th boosterDay 98Boost with 0.50 mg antigen in IFA, ID 20 sites
7th boosterDay 112Boost with 0.50 mg antigen in IFA, ID 20 sites
Serum collectionDay 112Test-bleed (~5mL) per goat
ELISA and shippingDay 118ELISA titration (results available online);
Verify disposition of goats; decide to continue or terminate;
Plasmapheresis from one goat (~1,000 mL serum),
taken when requested by customer
Instruction due dateDay 120If no animal instructions are received, per diem charges will apply;
Extension protocol is recommended with titer monitoring by ELISA

Deliverables: 22 vials crude antibody sera

  • 2 vials: 1–2 mL (1 per animal) test aliquots pre-immune sera
  • 2 vials: 5 mL (1 per animal) per vial pre-immune sera
  • 8 vials: 1–2 mL (4 per animal) test aliquots post-inject sera (Day 42, 56, 84, 112)
  • 8 vials: 5 mL per vial post-inject sera (Day 42, 56, 84, 112)
  • 1 vial: 1–2 mL test aliquot post-inject plasma (Plasmapheresis of one goat)
  • 1 vial: 1,000 mL bulk post-inject plasma (Plasmapheresis of one goat)