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Immunization and serum-collection procedures for custom goat polyclonal antibody production

Our comprehensive array of Thermo Scientific Pierce custom antibody services includes a customizable option for goat immunization, serum-collection and titer analysis for goat antibody production, plus several extension possibilities.

Custom Antibody Request

Highlights

  • Custom goat antibodies - a reliable protocol for immunizing goats, collecting serum, testing bleeds, reporting titer results and delivering antisera
  • Track and customize project - easily add additional goats to the default protocol; track and manage projects online with OpenProject Tool: you decide which animals to continue or terminate (see Extensions below)
  • Full service integration - combine this immunization service with our complete set of services for antigen preparation and antibody purification (see Custom Polyclonal Antibodies pages for details)
2-goat 120-day protocol

This goat immunization schedule uses two mixed-breed goats (usually Boer/Nubian or Alpine, unless specifically requested) for each MAP-peptide, conjugated-peptide, or protein immunogen. Injections are intradermal (ID) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used if requested.

120-day 2-goat immunization protocol for goat polyclonal antibody production

Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Injection amounts are given for a conjugated peptide antigen (e.g., KLH), MAP-peptide, or protein immunogen. Protocol Days are approximate (± 2 days).

Procedure Protocol day Description
Control serum collection Day 0 Pre-immune bleed (~5 mL per goat)
Primary injection Day 1 Immunize with 0.50 mg antigen in CFA, ID 20 sites
1st booster Day 14 Boost with 0.50 mg antigen in IFA, ID 20 sites
2nd booster Day 28 Boost with 0.50 mg antigen in IFA, ID 20 sites
Serum collection Day 42 Test-bleed (~5 mL) per goat
3rd booster Day 56 Boost with 0.50 mg antigen in IFA, ID 20 sites
Serum collection Day 56 Test-bleed (~5mL) per goat
4th booster Day 70 Boost with 0.50 mg antigen in IFA, ID 20 sites
5th booster Day 84 Boost with 0.50 mg antigen in IFA, ID 20 sites
Serum collection Day 84 Test-bleed (~5 mL) per goat
6th booster Day 98 Boost with 0.50 mg antigen in IFA, ID 20 sites
7th booster Day 112 Boost with 0.50 mg antigen in IFA, ID 20 sites
Serum collection Day 112 Test-bleed (~5mL) per goat
ELISA and shipping Day 118 ELISA titration (results available online);
Verify disposition of goats; decide to continue or terminate;
Plasmapheresis from one goat (~1100 mL serum), 
taken when requested by customer
Instruction due date Day 120 If no animal instructions are received, per diem charges will apply;
Extension protocol is recommended with titer monitoring by ELISA

Deliverables: 22 vials crude antibody sera

  • 2 vials: 1-2 mL test aliquots pre-immune sera
  • 2 vials: 5 mL per vial pre-immune sera
  • 8 vials: 1-2 mL test aliquots post-inject sera (Day 42, 56, 84, 112)
  • 8 vials: 5 mL per vial post-inject sera (Day 42, 56, 84, 112)
  • 1 vial: 1-2 mL test aliquot post-inject sera (Plasmapheresis of one goat)
  • 1 vial: 1100 mL bulk post-inject sera (Plasmapheresis of one goat)
Protocol extensions

Several pre-defined (yet customizable) options are available for extending or continuing the goat immunization and serum-collection schedule. The standard production bleed yields 250mL of goat serum per animal; plasmapheresis yields ~1100mL of serum per goat. The purchase decision to continue antibody production can be made at any time before scheduled termination.

One-month extension protocol for goat antibody production

Procedure Protocol day Description
1st booster Day 1 Boost with 0.50 mg antigen in IFA, 20 ID sites
Serum collection Day 14 Test-bleed (5 mL per goat)

Deliverables (per goat): 2 vials crude antibody sera

  • 1 vial: 5 mL post-inject sera
  • 1 vial: 1-2 mL test aliquot post-inject sera

One-month extension with production bleed protocol for goat antibody production

Procedure Protocol day Description
1st booster Day 1 Boost with 0.50 mg antigen in IFA, 20 ID sites
Serum collection Day 14 Bleed (250 mL per goat)

Deliverables (per goat): 2 vials crude antibody sera

  • 1 vial: 250 mL post-inject sera
  • 1 vial: 1-2 mL test aliquot post-inject sera

One-month extension with plasmapheresis protocol for goat antibody production

Procedure Protocol day Description
1st booster Day 1 Boost with 0.50 mg antigen in IFA, 20 ID sites
Serum collection Day 14 Plasmapheresis (1100 mL per goat)

Deliverables (per goat): 2 vials crude antibody sera

  • 1 vial: 1100 mL post-inject sera
  • 1 vial: 1-2 mL test aliquot post-inject sera

Aggressive, one-month extension protocol for goat antibody production

Procedure Protocol day Description
1st booster Day 1 Boost with 0.50 mg antigen in IFA, 4 SQ sites
2nd booster Day 7 Boost with 0.50 mg antigen in IFA, 4 SQ sites
Serum collection Day 21 Test-bleed (~5 mL per goat)

Deliverables (per goat): 2 vials crude antibody sera

  • 1 vial: 5 mL post-inject sera
  • 1 vial: 1-2 mL test aliquot post-inject sera
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