Aberrant autophagy is implicated in many important disease states such as cancer, neurodegeneration, and immunological diseases, and therefore small molecules that can remedy these deficiencies hold great therapeutic promise. The ability to study autophagosome formation and other aspects of autophagy, and to observe the effects of small molecules and gene manipulation on them in high throughput using physiologically relevant cell models, is of great importance, not only in understanding autophagy but also in producing effective treatments.
Autophagy describes the segregation and delivery of cytoplasmic cargo, including proteins and organelles, for degradation by hydrolytic enzymes through the lysosomal machinery. It is critical to the healthy functioning of cells and the failure of autophagy is a major reason for the accumulation of cell damage and aging.
The LC3 Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high-content imaging and analysis.
Autophagy detected in HeLa cells using anti-LC3B and Alexa Fluor® 488 goat anti–rabbit IgG.
|Target||LC3B associates with the phagophore early in autophagy|
|Readout||Localization of LC3B by fluorescence imaging|
|Fluorescent label||Requires secondary detection|
|Target protein||LC3B associates with the phagophore early in autophagy|
|Readout||Accumulation of LC3B-II measured in cell lysates using Tb-based TR-FRET|
|Fluorescent label||GFP and Tb-labeled LC3B-II detection antibody|
|Standard filter set||LanthaScreen®-specific instrument settings|
|Format||1 kit (333 tests in 96-well format)|
|Molecular Probes® Handbook