Dynabeads Oligo(dT)25 are optimal for mRNA isolation in expression profiling applications.
The hybridisation kinetics between mRNA and the Dynabeads are not only fast and efficient, but also comparable to that of free solution hybridisation. This ensures negligible sample loss.
The many types of arrays/chips commercially available require different quantities of mRNA. With Dynabeads, the possibility of scaling the isolation protocols allows you to isolate mRNA from any quantity of cells. This approach can therefore be used to prepare the amount of mRNA required for any type of micro- and macroarrays or chips. A few selected examples are; the isolation of mRNA for GeneChips arrays (Affymetrix Inc.) (1,2), for Gene Expression Microarray™ (Incyte Genomics Inc) (3, 4), and for different types of self-spotted arrays (5-11). Dynabeads can also be applied directly in certain electronic chips or microfludic devices (12).
Figure 1. Functional genomics approach using Dynabeads to find target genes in testicular cancer. Left: Microarray using input mRNA, shows either up- (red) or down- (green) regulated genes in tumour relative to normal testis. Right: Two of the genes were also validated as upregulated at their protein level. Courtesy of R. Skotheim and R. A. Lothe, the Norwegian Radium Hospital, Oslo, Norway.
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