Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The following cell line-specific conditions are provided as a starting point for transfecting wild-type cells with TrueGuide Synthetic gRNA and TrueCut Cas9 Protein v2 using the Lipofectamine CRISPRMAX Transfection Reagent. Further optimization of the transfection conditions may be necessary for best results.
NOTE: Scroll to the right within the table to see all data.
Cell type | Source | Media | Cell seeding density/well (× 103) one day before transfection | TrueCut Cas9 Protein v2/gRNA (ng/pmoles) | Lipofectamine Cas9 Plus Reagent/well (μL) | Lipofectamine CRISPRMAX Reagent/well (μL) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Well format | — | — | 96-well | 24-well | 6-well | 96-well | 24-well | 6-well | 96-well | 24-well | 6-well | 96-well | 24-well | 6-well |
HEK293 | Human embryonic kidney | DMEM | 18 | 90 | 450 | 250/1.5 | 1250/7.5 | 6250/37.5 | 0.5 | 2.5 | 12.5 | 0.4 | 2 | 10 |
U2OS | Human osteosarcoma | McCoy5A | 10 | 50 | 250 | 250/1.5 | 1250/7.5 | 6250/37.5 | 0.5 | 2.5 | 12.5 | 0.3 | 1.5 | 7.5 |
A549 | Human epithelial lung carcinoma | DMEM | 10 | 50 | 250 | 250/1.5 | 1250/7.5 | 6250/37.5 | 0.5 | 2.5 | 12.5 | 0.3 | 1.5 | 7.5 |
THP1 | Human peripheral blood monocyte leukemia | RPMI | 10 | 50 | 250 | 400/2.4 | 2000/12 | 10000/60 | 0.8 | 4 | 20 | 0.3 | 1.5 | 7.5 |
K562* | Human leukemia bone marrow | RPMI | 10 | 50 | 250 | 250/1.5 | 1250/7.5 | 6250/37.5 | 0.5 | 2.5 | 12.5 | 0.3 | 1.5 | 7.5 |
iPSC* | Human induced pluripotent stem cells | Essential 8 | 8 | 40 | 200 | 300/2 | 1500/10 | 7500/50 | 0.6 | 3 | 15 | 0.3 | 1.5 | 7.5 |
HepG2 | Human hepatocellular carcinoma | DMEM | 10 | 50 | 250 | 250/1.5 | 1250/7.5 | 6250/37.5 | 0.5 | 2.5 | 12.5 | 0.3 | 1.5 | 7.5 |
MDA- MB231 | Human epithelial (breast) adenocarcinoma | DMEM | 10 | 50 | 250 | 250/1.5 | 1250/7.5 | 6250/37.5 | 0.5 | 2.5 | 12.5 | 0.3 | 1.5 | 7.5 |
N2A | Mouse brain neuroblastoma | DMEM | 10 | 50 | 250 | 250/1.5 | 1250/7.5 | 6250/37.5 | 0.5 | 2.5 | 12.5 | 0.3 | 1.5 | 7.5 |
*Use the Neon Transfection System for higher editing efficiency. |
For Research Use Only. Not for use in diagnostic procedures.