The Specificity and Sensitivity of TaqMan® Assays—Now Available for Protein Research

Revolutionary TaqMan® Protein Assays combine the sensitive, specific protein-binding capabilities of antibodies with the superior relative quantitation capabilities of 5' nuclease real-time PCR. They enable fast, easy identification and relative quantification of proteins from small sample sizes or limited numbers of cells-all in a homogeneous assay format. With the TaqMan® Protein Assays Open Kit, you can make your own TaqMan® Protein Assay from biotinylated antibodies for your protein of interest.

  • Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
  • Provides greater sensitivity and a more quantitative readout than western blots and immunostaining—no fixing or staining cells
  • TaqMan® Protein Assays Open Kit enables simple assay development from biotinylated antibodies for your proteins of interest—even using a single polyclonal antibody

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New to TaqMan® Chemistry–Based Protein Assays? Start Here!

Select Your Area of Interest:

Protein Analysis on Small Samples

Determine protein expression in small samples such as stem cells or germ cell tumors.

Analysis of Post-Translational Modifications

Characterize expression of endogenous phosphorylation sites in samples.

Validation of Gene Transfection/Transduction

Confirm protein expression or characterize induced pluripotent stem cell (iPSC) colonies.

Correlation of RNA and Protein Results

Helpful for validating results from mRNA and/or microRNA (miRNA) assays.

Validation of siRNA Induced Silencing

Confirm knockdown of gene targets with siRNAs.

Experimental Workflow: Find the Products You Need for Every Step

Assays are built from an immunogen-purified polyclonal antibody preparation that is split into two pools or from matched antibody pairs suitable for ELISA, typically made up of one polyclonal antibody and one monoclonal antibody.

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TaqMan® Protein Assays Open Kit

Lyse cells at a concentration of 1,000 cells/µL cell lysis solution.

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Protein Expression Sample Preparation Kit

The assay probes are target-specific antibodies that are conjugated to oligonucleotides through a biotin-streptavidin linkage. Each oligonucleotide in the probe pair presents a 5' or 3' end; these ends are brought into proximity when the antibody components of the assay probe pair bind to two different epitopes on the target protein.

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TaqMan® Protein Assay Kit

The substrate for ligase is a bridge structure formed by hybridization of a third oligonucleotide to the oligonucleotide ends of the assay probe pair. This structure forms preferentially when the assay probes are in proximity to each other. Subsequent protease treatment inactivates the ligase.

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TaqMan® Protein Expression Core Reagents Kit with Master Mix

The ligation product serves as a template for real-time PCR using a TaqMan® Assay for protein detection and relative quantitation.

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StepOnePlus™ Real-Time PCR System
7500 Fast Real-Time PCR System
7900HT Fast Real-Time PCR System
ViiA™ 7 Real-Time PCR System

Ct values from the instrument data files are imported into the data analysis oftware for relative quantitation fold change results.

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ProteinAssist™ Software