SNP Genotyping Troubleshooting
I am having problems with...
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You cannot amplify a SNP. | |
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Not using ROX or not designating it as a passive reference dye may cause trailing clusters. | |
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You have diffuse clustering in your SNP analysis. | |
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Failure of autocalling of SNP results is usually due to outlier wells or software problems. | |
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Normal Allelic Discrimination Plot Example Your cluster plot should look something like this. |
For Research Use Only. Not for use in diagnostic procedures.