An allelic discrimination plot, also known as a "cluster plot" or an "AD plot" is shown below. Ideally, these plots show three clusters, and near the origin, the No Template Control (NTC). These clusters are described in the table below. The points in each cluster are grouped closely together, and each cluster is well separated from the other clusters.
A Typical three-cluster allelic discrimination plot
Assignment of clusters in an allelic discrimination plot
|Samples Containing...||Are grouped in...|
|Allele X (homozygote), labeled with VIC® dye||Lower right corner of the plot|
|Allele Y (homozygote), labeled with FAM™ dye||Upper left corner of the plot|
|Both (allele X and allele Y—heterozygote)||Approximately midway between the allele X and allele Y clusters|
|No template control (NTC)||Bottom left corner of the plot|
|Undetermined||Anywhere on the plot|
For Research Use Only. Not for use in diagnostic procedures.