The following procedure will enable you to accurately determine the cell viability. Cell viability is calculated as the number of viable cells divided by the total number of cells within the grids on the hemacytometer. If cells take up trypan blue, they are considered non-viable.
% viable cells = [1.00 – (Number of blue cells ÷ Number of total cells)] × 100
To calculate the number of viable cells per mL of culture, use the formula below. Remember to correct for the dilution factor.
Number of viable cells × 104 × 1.1 = cells/mL culture
For Research Use Only. Not for use in diagnostic procedures.