Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
This video presents the best way to thaw cells without harming them in this stressful process. Our scientists demonstrate how to carefully transfer cells from storage in liquid nitrogen to the incubator.
Prepare to thaw your cells by gathering the following materials:
The following protocol describes a general procedure for how to thaw cells. For detailed protocols, always refer to the cell-specific product insert. When thawing, always use proper aseptic technique and work in a laminar flow hood. Remember to wear personal protective equipment, including a face mask or goggles.
NOTE: Use caution when thawing cryovials stored in liquid-phase, they present a risk of explosion.
Some freezing media contain DMSO, which facilitates the entry of organic molecules into tissues. Handle reagents containing DMSO using equipment and practices appropriate for the hazards posed by such materials.
Note: The appropriate flask size depends on the number of cells frozen in the cryovial, and the culture environment varies based on the cell and media type.
Learn more about seeding, subculturing, and maintaining cells
See the protocol for subculturing adherent cells
See the protocol for subculturing suspension cells
The purpose of thawing cells is to effectively revive them from a frozen state with minimal damage. Proper techniques and troubleshooting common issues like slow recovery or contamination are essential for maintaining cell viability.
Concern | Recommendation |
---|---|
Cells were stored incorrectly |
|
Homemade freezer stock is not viable |
|
Cells were thawed incorrectly |
|
Thawing medium is not correct |
|
Cells are too dilute |
|
Cells not handled gently |
|
Glycerol used in the freezing medium was stored in light (if applicable) |
|
Grow and maintain a variety of mammalian cell lines with Gibco Cell Culture Media.
Nunc Cell Culture Plastics support every step of your research with a broad range of culture vessels.
Gibco FBS offers consistent growth for your cells through essential nutrients and growth factors.
Access cell culture and transfection educational resources to enable excellent experiment planning and execution.
Learn best practices and fundamentals of cell culture. Topics include aseptic technique and methods for the growth and maintenance of cells in culture.
Explore this collection of cell culture videos providing valuable insights into techniques, resources, and stay up to date with the latest advancements in the field.
Find the right cell culture products for your research with these selection tools. Search for media, FBS, plastics, transfection reagents, and more.
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
Contact our expert team for technical and application support of Laboratory Products.
For Research Use Only. Not for use in diagnostic procedures.