Which Human B Cell Isolation Product is Right for You?

  Highest purity for molecular assays   Pure flow compatible cells   Untouched—most physiological relevant cells
 
 
 
 
 
 
Isolation Method Depletion or positive isolation   Positive isolation   Negative isolation
Starting samples Any sample (whole blood, MNC, bone marrow)   Any sample (whole blood, MNC, bone marrow)   MNC*
# MNC processed
(mL whole blood processed)
1–2 x 109 MNC
200 mL whole blood
  1–2 x 109 MNC
80 mL whole blood
  1 x 109 cells
Purity Highest
98-100%
  Highest
98-100%
  High
90-95%
Yield Highest
95-100%
  Standard
50-70%
  Standard
50-70%
Viability >95%   >95%   >95%
Isolation Time ~30 min   ~90 min   ~50 min
Isolation Temp. Cold   Release at room temp.   Cold
Applications Depletion of unwanted B cells or positive isolation for molecular applications, e.g. isolation of proteins & nucleic acids   Cellular assays (cell culture, cell activation/proliferation, cytokine studies)
Translational research

Molecular: isolation of proteins & nucleic acids
  Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells)
Bead-free Target Cells No   Yes (and antibody-free)   Yes
Compatible with Flow Cytometry/Sorting No   Yes   Yes
Shelf Life from Production Date 3 years   3 years   2 years
Required Materials Magnets   Magnets   Magnets
  Order Now   Order Now   Order Now

*MNC—Mononuclear cells
** The only bead-based positive isolation method that gives both bead-and antibody-free cells

THIS IS FOR HUMAN RESEARCH—NOT FOR MOUSE