Selection guide for human T cell isolation

Which human T cell isolation kit is right for your application and sample type? Dynabeads has kits optimized for specific sample types broadly for whole blood, mononuclear cells (MNC) and bone marrow, or for those focused directly on MNC.

  Highest purity for molecular assays   Affordable & pure, flow-compatible cells   Extreme purity & physiological quality   Untouched—most physiological relevant cells
       
Isolation method Depletion or positive isolation   Depletion or positive isolation   Positive isolation   Negative isolation
Starting samples Any sample (whole blood, MNC, bone marrow)   Any sample (whole blood, MNC, bone marrow)   Any sample (whole blood, MNC, bone marrow)   MNC
# MNC processed (mL whole blood processed) 1-2 x 109 MNC
200 mL whole blood
  1-2 x 109 MNC
200 mL whole blood
  2 x 109 MNC
80 mL whole blood
  1 x 109 cells
Purity Highest
98-100%
  Highest
98-100%
  Higher
95-99%
  High
90-95%
Yield Highest
95-100%
  Highest
95-100%
  High
70-85%
  Standard
50-70%
Viability >95%   >95%   >95%   >95%
Isolation time ~30 min   ~30 min   ~60 min   ~50 min
Isolation temp. Cold   Cold   Cold   Cold
Applications Depletion of unwanted CD2+ T cells or positive isolation for molecular applications, e.g., isolation of proteins & nucleic acids   Depletion of unwanted CD3+ T cells or positive isolation for molecular applications, e.g., isolation of proteins & nucleic acids   Cellular assays (cell culture, cell activation/proliferation, cytokine studies)

Molecular isolation of proteins & nucleic acids
  Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells)
Bead-free target cells No   No   Yes (but antibody present)   Yes
Compatible with
flow cytometry/sorting
No   No   Yes   Yes
Shelf life from production date 3 years   3 years   2 years   2 years
Required materials Magnets   Magnets   Magnets   Magnets
  Order now   Order now   Order now   Order now



THIS IS FOR HUMAN RESEARCH - NOT FOR MOUSE