Tumor Cell Isolation
Proven Magnetic Separation Technology for Tumor Cells
Dynabeads® are used within a variety of applications within the field of cancer research, ranging from detecting circulating tumor cells to expanding T cells in immunotherapy.
Circulating Tumor Cell Detection
- Up to 5 log enrichment of tumor cells
- Detect 1 tumor cell per ml of blood
- Obtain viable cells for analysis by applications including staining, FISH, PCR or RT-PCR.
Isolate and detect minimal residual cancer cells with Dynabeads®. These easy-to-use products quickly provide clear, sensitive and accurate results. The tube-based method is extremely gentle to cells, ensuring high cell viability and yield.
Tumour cell detection is a two part procedure:
- Enrich tumor cells directly from whole blood, bone marrow or MNC samples
- Analyze enriched tumor cells for characterization and confirmation
You can enrich tumour cells using Dynabeads® coated with antibodies against specific cell markers. Add Dynabeads® to the starting sample, which then bind to the target cells. Separate the bead-bound cells with a magnet ( DynaMag™). The product you choose will depend on your chosen downstream application.
Enriched tumor cells for molecular analysis:
Use Dynabeads® Epithelial Enrich to positively isolate epithelial tumor cells from whole blood or bone marrow samples. After enrichment, obtain pure mRNA from the cells with Dynabeads® mRNA DIRECT™ Kit.
Use CELLection™ Epithelial Enrich to enrich tumor cells, then remove the beads from the cells. The bead-free cells can be used for any downstream application.
Dynabeads® FlowComp™ Flexi, CELLection™ Pan Mouse IgG and CELLection™ Biotin Binder can also be used in combination with your own tumor-specific antibodies.
Deplete leucocytes from your sample with Dynabeads® CD45 to leave untouched tumor cells that can be used for any downstream application.
Human and Mouse T Cell Expansion
Bridge the gap from mouse to human models with the equivalent product for expanding mouse cells, Dynabeads® Mouse CD3/CD28 T Cell Expander .