Microscopy Protocols

Find step-by-step instructions for your imaging experiments. If you are just starting, we recommend the Molecular Probes School of Fluorescence as a starting point. Protocols linked below are to be used as a guide only, and may require optimization for your experiment.

Cell proliferation protocols for imaging

• BrdU Labeling and Detection Protocol
• Click-iT EdU HCS Assays Protocol
• Click-iT EdU Imaging Kits Protocol
• Click-iT Plus EdU Imaging Kits Protocol
• Click-iT EdU and Click-iT Plus EdU for In Vivo Protocol

Cell viability protocols for imaging

• Image-iT DEAD Green Kit Protocol
  
• LIVE BacLight Bacterial Gram Stain Kit Protocol
• LIVE/DEAD BacLight Bacterial Viability Kit Protocol
• LIVE/DEAD Cell Imaging Kit (488/570) Protocol
  
• LIVE/DEAD Sperm Viability Kit Imaging Protocol
• LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells Protocol
  
• LIVE/DEAD Yeast Viability Kit Protocol
• NucGreen Dead 488 ReadyProbes Reagent Protocol for Viability
  
• NucRed Dead 647 ReadyProbes Reagent Protocol for Viability
  
• ReadyProbes Cell Viability Imaging Kit, Blue/Green Protocol
  
• ReadyProbes Cell Viability Imaging Kit, Blue/Red Protocol

Cell structure protocols for imaging

• ActinGreen 488 ReadyProbes Reagent Protocol
• ActinRed 555 ReadyProbes Reagent Protocol
• DAPI Protocol for Fluorescence Imaging
  
• F-Actin Labeling with Fluorescent Phalloidin Protocol
  
• Hoechst 33342 Protocol for Imaging
• Functional Mitochondrial Staining with TMRM Protocol
• NucBlue Fixed Cell ReadyProbes Reagent Protocol
• NucBlue Live ReadyProbes Reagent Protocol
• NucGreen Dead 488 ReadyProbes Reagent Protocol for Fixed Cells
  
• NucRed Dead 647 ReadyProbes Reagent Protocol for Fixed Cells
• NucRed Live 647 ReadyProbes Reagent Protocol
• SYTO 59 Nuclear Staining Protocol
• SYTO 82 Nuclear Staining Protocol
• SYTOX Green Nucleic Acid Stain Protocol
• TO-PRO-3 Stain Protocol

General antibody staining protocols

• Automated Multiplex IHC Staining with Aluora Spatial Amplification Kits and Leica BOND
• Immunofluorescent Staining of Intracellular Antigens on Cultured Cells (Invitrogen eBioscience reagents)
• IHC Frozen Tissue—Direct Method (Invitrogen eBioscience reagents)
• IHC Frozen Tissue—Indirect Method (purified) (Invitrogen eBioscience reagents)
• IHC Frozen Tissue—Indirect Method (biotin) (Invitrogen eBioscience reagents)
• IHC FFPE Tissue Trypsin Digestion—Direct Method (Invitrogen eBioscience reagents)
• IHC FFPE Tissue Trypsin Digestion Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
• IHC FFPE Tissue Low pH Antigen Retrieval—Direct Method (Invitrogen eBioscience reagents)
• IHC FFPE Tissue Low pH Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
• IHC FFPE Tissue High pH Antigen Retrieval—Direct Method (Invitrogen eBioscience reagents)
• IHC FFPE Tissue High pH Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
• IHC Staining of Formalin-Fixed Paraffin-Embedded Tissues (Invitrogen eBioscience reagents)
• Colorimetric FFPE—High pH Antigen Retrieval (Invitrogen eBioscience reagents)
• Colorimetric FFPE—Low pH Antigen Retrieval (Invitrogen eBioscience reagents)
• Colorimetric FFPE—Trypsin Digestion (Invitrogen eBioscience reagents)
• ICC Formaldehyde Fixed Cells—Direct Method (Invitrogen eBioscience reagents)
• ICC Formaldehyde Fixed Cells—Indirect Method (Invitrogen eBioscience reagents)
• ICC Formaldehyde Fixed, Permeabilized Cells—Direct Method (Invitrogen eBioscience reagents)
• ICC Formaldehyde Fixed, Permeabilized Cells—Indirect Method (Invitrogen eBioscience reagents)
• ICC Methanol Fixed Cells—Direct Method (Invitrogen eBioscience reagents)
• ICC Methanol Fixed Cells—Indirect Method (Invitrogen eBioscience reagents)
• ICC Unfixed Cells—Direct Method (Invitrogen eBioscience reagents)
• Mounting Coverslips Protocol
• Simple Immunolabeling with Primary and Secondary Antibodies Protocol
• Tyramide staining for tissue samples
• 3D Organoid Staining
• ViewRNA tissue fluorescence assay protocol
• ViewRNA tissue colorimetric assay protocol

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