Which Human B Cell Isolation Product is Right for You?

  Highest purity for molecular assays Pure flow compatible cells Untouched—most physiological relevant cells
Isolation MethodDepletion or positive isolationPositive isolationNegative isolation
Starting samplesAny sample (whole blood, MNC, bone marrow)Any sample (whole blood, MNC, bone marrow)MNC*
# MNC processed
(mL whole blood processed)
1–2 x 109 MNC
200 mL whole blood
1–2 x 109 MNC
80 mL whole blood
1 x 109 cells
PurityHighest
98-100%
Highest
98-100%
High
90-95%
YieldHighest
95-100%
Standard
50-70%
Standard
50-70%
Viability>95%>95%>95%
Isolation Time~30 min~90 min~50 min
Isolation Temp.ColdRelease at room temp.Cold
ApplicationsDepletion of unwanted B cells or positive isolation for molecular applications, e.g. isolation of proteins & nucleic acidsCellular assays (cell culture, cell activation/proliferation, cytokine studies)
Translational research

Molecular: isolation of proteins & nucleic acids
Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells)
Bead-free Target CellsNoYes (and antibody-free)Yes
Compatible with Flow Cytometry/SortingNoYesYes
Shelf Life from Production Date3 years3 years2 years
Required MaterialsMagnetsMagnetsMagnets
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*MNC—Mononuclear cells
** The only bead-based positive isolation method that gives both bead-and antibody-free cells

THIS IS FOR HUMAN RESEARCH—NOT FOR MOUSE